| Project/Area Number |
22591611
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KUROZUMI Kazuhiko 岡山大学, 大学院・医歯薬学総合研究科, 講師 (20509608)
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| Co-Investigator(Renkei-kenkyūsha) |
DATE Isao 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70236785)
MIYOSHI Yasuyuki 岡山大学, 岡山大学病院, 講師 (00362997)
ONO Shigeki 川崎医科大学, 医学部, 教授 (40335625)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 腫瘍幹細胞 |
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| Research Abstract |
Invasion, angiogenesis, and proliferation of malignant gliomas are fundamental traits and major reasons for treatment failure. These traits are considered to originate from glioma stem cell niche. Delineation of glioma stem cell niche is important in establishing treatment for gliomas. We have established two animal models that histologically recapitulated two invasive and angiogenic phenotypes, namely angiogenesis-dependent (J3T-1) and .independent (J3T-2) invasion, also observed in human glioblastoma. GFP-expressing J3T-1G cells were inoculated to establish brain tumors in athymic rats. Pathological samples of these animal gliomas were examined to analyze proliferation, invasioin, and angiogenesis in relation to glioma stem cell niche. J3T-1G tumor showed remarkable angiogenesis, and glioma cells clustered around newly developed vessels at tumor borders, For dynamic analysis, slice culture of rat brain harboring glioma was monitored by timelapse imaging. Cell division of J3T-1G cells were seen around vasculature. Tumor cells migrated along external space of vascular channel. Our results indicated glioma niche exists around vasculature. Microenvironment of glioma stem cell niche was shown histologically.
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