Functional analysis of genomic abnormalities in brain tumors by epigenetic reprogramming
| Project/Area Number |
22591614
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kochi University |
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Principal Investigator |
YAWATA Toshio 高知大学, 教育研究部医療学系, 助教 (40380323)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMIZU Keiji 高知大学, 教育研究部医療学系, 教授 (70116044)
HIGASHI Yoichiro 高知大学, 教育研究部医療学系, 助教 (80380062)
TAMURA Masakazu 高知大学, 教育研究部医療学系, 講師 (40516150)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 脳腫瘍 / エピジェネティクス / ゲノムリプログラミング / 悪性脳腫瘍 / 分子生物学 / 変異 |
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| Research Abstract |
To establish induced pluripotent stem (iPS) cell line derived from brain tumor, NANOG promoter-GFP, SOX2, KLF4 and OCT3/4 genes were introduced into glioma cells. After the induction of iPS cells, alkaline phosphatase-positive clones were appeared but failed to grow continuously. This result suggests that altered or mutated genes in glioma cells might be required for genome reprogramming and maintenance of pluripotency in embryonic stem-like cells.
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Report
(4 results)
Research Products
(67 results)
