Therapeutic effects of transplanted peripheral blood mononuclear cells which mobilized by G-CSF on spinal cord injury in mice
Project/Area Number |
22591626
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Orthopaedic surgery
|
Research Institution | University of Tsukuba (2012) Chiba University (2010-2011) |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
OKAWA Akihiko 千葉大学, 大学院・医学研究院, 講師 (30312945)
MURATA Atsushi 千葉大学, 医学部附属病院, 准教授 (20344997)
FURUYA Takeo 千葉大学, 医学部附属病院, 医員 (00507337)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | 脊椎脊髄病学 / 脊髄損傷 / G-CSF / 細胞移植 |
Research Abstract |
We analyzed that intraspinal transplantation of CD34+ peripheral blood cells mobilized by granulocyte colony-stimulating factor (G-CSF) facilitates angiogenesis in injured spinal cord and promotes functional recovery after spinal cord injury (SCI). NOD-Scid mice were subjected to contusion-induced SCI. Human CD34+ peripheral blood stem cells (1.0x10^5) mobilized by G-CSF were injected into the injured spinal cords (CD34 group) one week after SCI. Human mononuclear cell fraction (1.0x10^5) mobilized by G-CSF (M group) or saline (Control group) were also administrated. Mice in CD34 and M groups showed significant locomotor recovery of hind limb motor function fromsix to eight weeks after the transplantation (p<0.01). A certain amount of transplanted cells were detected until four weeks, and they were differentiated into vascular endothelial cells. Those results indicate that intraspinal transplantation of human peripheral blood stem cells mobilized by G-CSF facilitates the angiogenesis in injured spinal cord and promotes functional recovery in mice.
|
Report
(4 results)
Research Products
(126 results)
-
-
-
-
-
[Journal Article] Granulocyte colony-stimulating factor (G-CSF) protects oligpdendrocyte and promotes hindlimb functional recovery after spinal cord injury in rats2012
Author(s)
Kadota R, Koda M, Kawabe J, HashimotoM, Nishio Y, Mannoji C, Miyashita T, Furuya T, Okawa A, Takahashi K, Yamazaki M
-
Journal Title
Related Report
Peer Reviewed
-
[Journal Article] Neuroprotective therapy using granulocyte-colony stimulating factor for patients with worsening symptoms of thoracic myelopathy, a multicenter prospective controlled trial2012
Author(s)
Sakuma T, Yamazaki M, Okawa A, Takahashi H, Kato K, Hashimoto M, Hayashi K, Furuya T, Fujiyoshi T, Kawabe J, Mannoji C, Miyashita T, Kadota R, Someya Y, Ikeda O, Yamauchi T, Hashimoto M, Aizawa T, Ono A, Imagama S, Kanemura T, Hanaoka H, Takahashi K, Koda
-
Journal Title
Spine.
Volume: 37(17)
Pages: 1475-1478
NAID
Related Report
Peer Reviewed
-
[Journal Article] Neuroprotective therapy using granulocyte colony-stimulating factor for acute spinal cord injury: a phase I/IIa clinical trial2012
Author(s)
Takahashi H, Yamazaki M, Okawa A, Sakuma T, Kato K, Hashimoto M, Hayashi K, Furuya T, Fujiyoshi T, Kawabe J, Yamauchi T, Mannoji C, Miyashita T, Kadota R, Hashimoto M, Ito Y, Takahashi K, Koda M
-
Journal Title
Eur Spine J
Volume: 21(12)
Pages: 2580-2587
Related Report
Peer Reviewed
-
[Journal Article] Neuroprotective therapy using granulocyte-colony stimulating factor for patients with worsening symptoms of compression myelopathy, part 1: a phase I and IIa clinical trial2012
Author(s)
Sakuma T, Yamazaki M, Okawa A, Takahashi H, Kato K, Hashimoto M, Hayashi K, Furuya T, Fujiyoshi T, Kawabe J, Mannoji C, Kadota R, Hashimoto M, Takahashi K, Koda M
-
Journal Title
Eur Spine J
Volume: 21(3)
Pages: 482-489
Related Report
Peer Reviewed
-
-
[Journal Article] 急性脊髄損傷および圧迫性脊髄症急性増悪例に対するG-CSF神経保護療法:医師主導型自主臨床試験2012
Author(s)
山崎正志,国府田正雄,佐久間毅,高橋宏,加藤 啓,橋本光宏,林 浩一,川辺純子,藤由崇之,古矢丈雄,山内友規,門田 領,萬納寺誓人,宮下智大,染谷幸男,鎌田尊人,池田 修,橋本将行,須田浩太,楫野知道,上田明希,伊藤康夫,植田尊善,相澤俊峰,小野 睦,今釜史郎,金村徳相,大河昭彦,高橋和久
-
Journal Title
日整会誌
Volume: 86
Pages: 891-896
NAID
Related Report
-
-
-
-
-
-
[Journal Article] Neuroprotective therapy using granulocyte colony-stimulating factor for acute spinal cord injury : a phase I/IIa clinical trial2012
Author(s)
Takahashi H, Yamazaki M, Okawa A, Sakuma T, Kato K, Hashimoto M, Hayashi K, Furuya T, Fujiyoshi T, Kawabe J, Yamauchi T, Mannoji C, Miyashita T, Kadota R, Hashimoto M, Ito Y, Takahashi K, Koda M
-
Journal Title
Eur Spine J
Volume: (Epub ahead of print)
Related Report
Peer Reviewed
-
[Journal Article] Neuroprotective therapy using granulocyte colony-stimulating factor for patients with worsening symptoms of compression myelopathy, part I : a phase I and IIa clinical trial2012
Author(s)
Sakuma T, Yamazaki M, Okawa A, Takahashi H, Kato K, Hashimoto M, Hayashi K, Furuya T, Fujiyoshi T, Kawabe J, Mannoji C, Kadota R, Hashimoto M, Takahashi K, Koda M
-
Journal Title
Eur Spine J
Volume: 21
Pages: 482-489
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] 急性脊髄損傷に対して顆粒球コロニー刺激因子(Granulocyte colony-stimulating factor : G-CSF)投与による神経保護療法を施行した6症例2010
Author(s)
高橋宏, 山崎正志, 大河昭彦, 国府田正雄, 橋本将行, 橋本光宏, 林浩一, 佐久間毅, 川辺純子, 藤由崇之, 古矢丈雄, 山内友規, 門田領, 宮下智大, 萬納寺誓人, 染谷幸男, 西尾豊, 鎌田尊人, 越塚周平, 池田修, 喜多恒次, 青木保親, 吉永勝訓, 村田淳, 高橋和久
-
Journal Title
千葉医学雑誌
Volume: 86
Pages: 175-183
NAID
Related Report
Peer Reviewed
-
[Journal Article] 圧迫性脊髄症の急性増悪期に顆粒球コロニー刺激因子(G-CSF)投与による神経保護療法を施行した5症例2010
Author(s)
佐久間毅, 山崎正志, 国府田正雄, 高橋宏, 林浩一, 川辺純子, 藤由崇之, 古矢丈雄, 山内友規, 門田領, 宮下智大, 萬納寺誓人, 染谷幸男, 西尾豊, 鎌田尊人, 越塚周平, 池田修, 喜多恒次, 安宅洋美, 吉永勝訓, 村田淳, 橋本将行, 橋本光宏, 大河昭彦, 高橋和久
-
Journal Title
千葉医学雑誌
Volume: 86
Pages: 11-18
NAID
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] 脊髄再生研究の最前線2010
Author(s)
国府田正雄, 橋本将行, 林浩一, 大河昭彦, 高橋和久, 山崎正志
-
Journal Title
J Spine Res
Volume: 1
Pages: 131-136
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Neuroprotective therapy using granulocyte-colony stimulating factor for patients with rapidly aggravating compression myelopathy : a phase I and IIa clinical trial2011
Author(s)
Sakuma T, Yamazaki M, Koda M, Okawa A, Hashimoto M, Hashimoto M, Mannoji M, Furuya T, Takahashi H, Kato K
Organizer
2nd Annual Meeting, Cervical Spine Research Society-Asia Pacific Section
Place of Presentation
Paradise Hotel Busan, Korea
Related Report
-
[Presentation] A phase I and IIa clinical trial using granulocyte colony-stimulating factor (G-CSF) as a neuroprotective therapy for acute spinal cord injury2011
Author(s)
Takahashi H, Yamazaki M, Okawa A, Koda M, Hashimoto M, Hashimoto M, Fujiyoshi T, Furuya T, Sakuma T, Kato K, Takahashi K
Organizer
2nd Annual Meeting, Cervical Spine Research Society-Asia Pacific Section
Place of Presentation
Paradise Hotel Busan, Korea
Related Report
-
[Presentation] C5 palsy following anterior decompression and spinal fusion for cervical degenerative diseases2011
Author(s)
Hashimoto M, Mochizuki M, Aiba A, Okawa A, Sakuma T, Takahashi H, Kato K, Takahashi K, Yamazaki M
Organizer
2nd Annual Meeting, Cervical Spine Research Society-Asia Pacific Section
Place of Presentation
Paradise Hotel Busan, Korea
Related Report
-
[Presentation] Granulocyte colony-stimulating factor (G-CSF) reduced neuropathic pain in patients with rapidly aggravating compression myelopathy : analyses of clinical trial cases2011
Author(s)
Kato K, Yamazaki M, Sakuma T, Takahashi H, Hashimoto M, Furuya T, Hashimoto M, Koda M, Okawa A, Takahashi K
Organizer
2nd Annual Meeting, Cervical Spine Research Society-Asia Pacific Section
Place of Presentation
Paradise Hotel Busan, Korea
Related Report
-
-
-
-
[Presentation] Granulocyte colony stimulating factor (G-CSF) reduced neuropathic pain in patients with rapidly aggravating compression myelopathy : analyses of clinical trial cases2011
Author(s)
Kato K, Yamazaki M, Sakuma T, Takahashi H, Hashimoto M, Furuya T, Hashimoto M, Koda M, Okawa A, Takahashi K
Organizer
27th Annual Meeting, Cervical Spine Research Society-European Section
Place of Presentation
Sheraton Atakoy hotel, Istanbul, Turkey
Related Report
-
-
-
[Presentation] Comparison of cervical OPLL classification between lateral radiographs and CT sagittal reconstruction images2011
Author(s)
Yamazaki M, Fujiyoshi T, Kamiya K, Furuya T, Sakuma H, Takahashi H, Kato K, Okawa A
Organizer
27th Annual Meeting, Cervical Spine Research Society-European Section
Place of Presentation
Sheraton Atakoy hotel, Istanbul, Turkey
Related Report
-
[Presentation] Neuroprotective therapy using granulocyte-colony stimulatingfactor for patients with rapidly aggravating compression myelopathy : a phase I and IIa clinical trial2011
Author(s)
Sakuma T, Yamazaki M, Okawa A, Koda M, Hashimoto M, Hashimoto M, Furuya T, Takahashi H, Kato K, Takahashi K
Organizer
27th Annual Meeting, Cervical Spine Research Society-European Section
Place of Presentation
Sheraton Atakoy hotel, Istanbul, Turkey
Related Report
-
[Presentation] Neuroprotective therapy using granulocyte colony-stimulating factor for acute spinal cord injury : a multicenter prospective controlled clinical trial2011
Author(s)
Takahashi H, Yamazaki M, Okawa A, Hashimoto M, Hashimoto M, Furuya T, Sakuma T, Kato K, Fujiyoshi T, Suda K, Kajino T, Ueda H, Ito Y, Ueta T, Takahashi K, Koda M
Organizer
39th Annual Meeting, Cervical Spine Research Society
Place of Presentation
The Phoenician, Scottsdale, Arizona, U.S.A.
Related Report
-
[Presentation] Neuroprotective therapy using G-CSF for patients with acute progressive compression myelopathy : a prospective case control study2011
Author(s)
Sakuma T, Yamazaki M, Koda M, Hashimoto M, Furuya T, Takahashi H, Kato K, Aizawa T, Ono A, Imagama S, Kanemura T, Takahashi K
Organizer
39th Annual Meeting, Cervical Spine Research Society
Place of Presentation
The Phoenician, Scottsdale, Arizona, U.S.A.
Related Report
-
[Presentation] Granulocyte colony stimulating factor (G-CSF) reduced neuropathic pain in patients with acutely progressing compression myelopathy : analyses of clinical trial cases2011
Author(s)
Kato K, Yamazaki M, Sakuma T, Takahashi H, Hashimoto M, Furuya T, Hashimoto M, Koda M, Okawa A, Takahashi K
Organizer
39th Annual Meeting, Cervical Spine Research Society
Place of Presentation
The Phoenician, Scottsdale, Arizona, U.S.A.
Related Report
-
-
-
-
[Presentation] 拡散強調MR neurographyによる頚神経根症および腕神経叢損傷の評価2011
Author(s)
江口和, 大鳥精司, 山崎正志, 国吉一樹, 井上玄, 折田純久, 鴨田博人, 新井玄, 石川哲大, 宮城正行, 鈴木都, 桝田喜正, 高橋和久
Organizer
第40回日本脊椎脊髄病学会
Place of Presentation
京王プラザホテル 東京都
Related Report
-
-
-
-
-
-
-
-
[Presentation] 胸椎後縦靭帯骨化症に対する各種固定術の成績:多施設後ろ向き研究2011
Author(s)
松本守雄, 辻崇, 千葉一裕, 戸山芳昭, 筑田博陸, 竹下克志, 加藤剛, 進藤重雄, 鐙邦芳, 高畑雅彦, 野原裕, 種市洋, 富田勝郎, 川原範夫, 今釜史郎, 若尾典充, 松山幸弘, 山崎正志, 大河昭彦
Organizer
第40回日本脊椎脊髄病学会
Place of Presentation
京王プラザホテル 東京都
Related Report
-
[Presentation] 拡散強調MRIを用いた腰部神経根障害の経時的評価2011
Author(s)
江口和, 大鳥精司, 山崎正志, 国吉一樹, 井上玄, 折田純久, 鴨田博人, 新井玄, 石川哲大, 宮城正行, 鈴木都, 桝田喜正, 高橋和久
Organizer
第40回日本脊椎脊髄病学会
Place of Presentation
京王プラザホテル 東京都
Related Report
-
-
[Presentation] 脊髄障害性疼痛に対する顆粒球コロニー刺激因子(G-CSF)の効果:臨床試験例での解析2011
Author(s)
加藤啓, 山崎正志, 佐久間毅, 高橋宏, 橋禾光宏, 林浩一, 古矢丈雄, 橋本将行, 国府田正雄, 大河昭彦, 高橋和久
Organizer
第40回日本脊椎脊髄病学会
Place of Presentation
京王プラザホテル 東京都
Related Report
-
-
-
[Presentation] 当科においてリネゾリドを使用した脊椎感染症例3の検討2011
Author(s)
鴨田博人, 大鳥精司, 江口和, 新井玄, 石川哲大, 宮城正行, 鈴木都, 佐久間詳浩, 久保田剛, 及川泰宏, 井上玄, 大河昭彦, 山崎正志, 高橋和久
Organizer
第40回日本脊椎脊髄病学会
Place of Presentation
京王プラザホテル 東京都
Related Report
-
[Presentation] 脊髄障害性疼痛に対する顆粒球コロニー刺激因子の治療効果2011
Author(s)
加藤啓, 国府田正雄, 山崎正志, 佐久間毅, 高橋宏, 林浩一, 古矢丈雄, 萬納寺誓人, 橋本将行, 大河昭彦, 高橋和久
Organizer
第26回日本整形外科学会基礎学術集会
Place of Presentation
群馬県民会館 前橋市
Related Report
-
[Presentation] 急性頚髄損傷に対する顆粒球コロニー刺激因子(G-CSF)を用いた神経保護療法:多施設前向き比較対照試験2011
Author(s)
高橋宏, 山崎正志, 大河昭彦, 藤由崇之, 染谷幸男, 鎌田尊人, 門田領, 林浩一, 須田浩太, 上田明希, 楫野知道, 伊藤康夫, 植田尊善, 国府田正雄, 高橋和久
Organizer
第26回日本整形外科学会基礎学術集会
Place of Presentation
群馬県民会館 前橋市
Related Report
-
[Presentation] マウス脊髄損傷モデルに対するG-CSF動員末梢血幹細胞移植の治療効果, 第2報2011
Author(s)
高橋宏, 国府田正雄, 橋本将行, 大河昭彦, 橋本光宏, 古矢丈雄, 佐久間毅, 加藤啓, 山崎正志, 高橋和久
Organizer
第26回日本整形外科学会基礎学術集会
Place of Presentation
群馬県民会館 前橋市
Related Report
-
[Presentation] 圧迫性脊髄症急性増悪例に対する顆粒球コロニー刺激因子(G-CSF)神経保護療法:多施設前向き比較対照試験2011
Author(s)
佐久間毅, 山崎正志, 国府田正雄, 加藤啓, 高橋宏, 古矢丈雄, 橋本光宏, 大河昭彦, 相澤俊峰, 小野睦, 今釜史郎, 金村徳相
Organizer
第26回日本整形外科学会基礎学術集会
Place of Presentation
群馬県民会館 前橋市
Related Report
-
[Presentation] 脊髄障害性疼痛に対するG-CSFの効果:医師主導型自主臨床試験例による解析2011
Author(s)
加藤啓, 山崎正志, 佐久間毅, 高橋宏, 橋本光宏, 林浩一, 古矢丈雄, 橋本将行, 国府田正雄, 大河昭彦, 高橋和久
Organizer
第26回日本整形外科学会基礎学術集会
Place of Presentation
群馬県民会館 前橋市
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-