| Project/Area Number |
22591663
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kagoshima University |
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Principal Investigator |
YAMAMOTO Takuya 鹿児島大学, 医学部・歯学部附属病院, 講師 (40381157)
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| Co-Investigator(Kenkyū-buntansha) |
SETOGUCHI Takao 鹿児島大学, 大学院・医歯学総合研究科, 特任准教授 (40423727)
小宮 節郎 鹿児島大学, 医歯(薬)学総合研究科, 教授 (30178371)
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| Co-Investigator(Renkei-kenkyūsha) |
KOMIYA Setsuro 鹿児島大学, 大学院・医歯学総合研究科, 教授 (30178371)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 骨肉腫 / Hedgehog Smoothened / 転移 / Hedgehog / GLI2 / 細胞増殖 / 細胞浸潤 / 細胞周期 |
|---|
| Research Abstract |
We have examined the function of Hedgehog pathway in osteosarcoma cell proliferation, metastasis, and cancer stem cells. We found activation of Hedgehog pathway in human osteosarcoma. Inhibition of Smoothened (SMO) prevented osteosarcoma cell proliferation by cell cycle regulation in vitro and in vivo. In addition, pharmacological inhibition of SMO or GLI prevents human rhabdomyosarcoma cell proliferation. Furthermore, inhibition of GLI2 prevents osteosarcoma cell proliferation, invasion, and lung metastasis. We have already found that other signal related molecule regulate the osteosarcoma cell metastasis by Hedgehog signal activation.
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