| Project/Area Number |
22591669
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nara Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TOHMA Yasuaki 奈良県立医科大学, 整形外科, 研究生 (60448777)
AKAHANE Manabu 奈良県立医科大学, 健康政策, 准教授 (40326327)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 同種骨移植 / 処理骨 / 間葉系細胞 / 骨形成 / 再生医療 / allograft / mesenchymal cell / hydroxyapatite / tri-calcium phospate / irradiation / osteogenesis / tissue engineering / angiogenesis / tri-calcium phosphate / PARP inhibitor / mesenchymal cel / bone graft / mesenchymal stem cell / vasculogenesis |
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| Research Abstract |
Our study aimed to determine whether in vivo osteogenesis could be enhanced using allogenic irradiated bone grafts after seeding with tissue-engineered autologous bone marrow-derived mesenchymal stem cells (BMSCs).
We used rat experimental model. The allogenic bone samples were extracted from ACI rat bone and sterilized by irradiation. Donor BMSCs were obtained from Fischer 344 rat bone marrow by cell culture. The allogenic bone with or without BMSCs were transplanted subcutaneously into syngenetic F344 rats. At 2, 4 weeks after transplantation, alkaline phosphatase activity, osteocalcin mRNA expression and histological examination were detected. These biochemical values were gradually increased. New bone was found in histological section. Furthermore, we studied the origin of the new bone. It was derived from cultured donor BMSCs after 4 weeks transplantation at least. These results indicate the availability of autologous tissue-engineered BMSCs to heighten the osteogenic response.
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