Identification and cell line establishment of human osteoclast precursor cell

• Project/Area Number: 22591690
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: Keio University
• Principal Investigator: FURUKAWA Mitsuru 慶應義塾大学, 医学部, 助教 (70468502)
• Co-Investigator(Kenkyū-buntansha): HORIUCHI Keisuke 慶應義塾大学, 医学部, 講師 (30327564)
• Co-Investigator(Renkei-kenkyūsha): MIYAMOTO Takeshi 慶應義塾大学, 医学部, 特任准教授 (70383768)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 破骨細胞 / 不死化 / Abelson virus / レトロウイルス / 細胞株 / 骨粗鬆症 / 治療薬

Research Abstract

Acquisition of cDNA of Abelson virus was tried and it succeeded in identification of a full length clone. When gene sequences were analyzed using the Direct sequence method, it was checked that there is no problem in gene sequences. A restriction enzyme s

Research Products

• [Journal Article] 脊椎疾患による痺れに対するプレガバリン投与の検討 (2013)
  • Author(s): 古川 満、鎌田 修博、蔵本 哲也、日方智宏、川崎 俊樹、千葉 和宏
  • Journal Title: 東日本整形災害外科 Volume: 25巻 Pages: 29-36
  • NAID: 10031161694
  • Peer Reviewed
• [Journal Article] 脊椎疾患による痺れに対するプレガバリン投与の検討 (2013)
  • Author(s): 古川 満
  • Journal Title: 東日本整形災害雑誌 Volume: ２５巻 Pages: 29-36
  • NAID: 10031161694
• [Journal Article] Risk factors for adjacent segment disease after posterior lumbar interbody fusion and efficacy of simultaneous decompression surgery for symptomatic adjacent segment disease (2012)
  • Author(s): Hikata T, Kamata M, Furukawa M
  • Journal Title: J Spinal Disord Tech Volume: (in press)
  • Peer Reviewed
• [Journal Article] An essential role for STAT6-STAT1 protein signaling in promoting macrophage cell-cell fusion (2012)
  • Author(s): Miyamoto H, Katsuyama E, Miyauchi Y, Hoshi H, Miyamoto K, Sato Y, Kobayashi T, Iwasaki R, Yoshida S, Mori T, Kanagawa H, Fujie A, Hao W, Morioka H, Matsumoto M, Toyama Y, Miyamoto T
  • Journal Title: J Biol Chem Volume: 287(39) Pages: 32479-84
  • Peer Reviewed
• [Journal Article] Conditional inactivation of Blimp1 in adult mice promotes increased bone mass. (2012)
  • Author(s): Miyauchi Y, Miyamoto H, Yoshida S, Mori T, Kanagawa H, Katsuyama E, Fujie A, Hao W, Hoshi H, Miyamoto K, Sato Y, Kobayashi T, Akiyama H, Morioka H, Matsumoto M, Toyama Y, Miyamoto T.
  • Journal Title: J Biol Chem Volume: 287(34) Pages: 28508-17
  • Peer Reviewed
• [Journal Article] Clinical Significance of Cartilage Biomarkers for Monitoring Structural Joint Damage in Rheumatoid Arthritis Patients Treated with Anti-TNF Therapy. (2012)
  • Author(s): Niki Y, Takeuchi T, Nakayama M, Nagasawa H, Kurasawa T, Yamada H, Toyama Y, Miyamoto T
  • Journal Title: PLoS One Volume: 7(5)
  • Peer Reviewed
• [Journal Article] Enhanced susceptibility to lipopolysaccharide-induced arthritis and endotoxin shock in interleukin-32 alpha transgenic mice through induction of tumor necrosis factor alpha (2012)
  • Author(s): Nakayama M, Niki Y, Kawasaki T, Takeda Y, Horiuchi K, Sasaki A, Okada Y, Umezawa K, Ikegami H, Toyama Y, Miyamoto T.
  • Journal Title: Arthritis Res Ther Volume: 14(3)
  • Peer Reviewed
• [Journal Article] PDGFBB promotes PDGFRα-positive cell migration into artificial bone in vivo (2012)
  • Author(s): Yoshida S, Iwasaki R, Kawana H, Miyauchi Y, Hoshi H, Miyamoto H, Mori T, Kanagawa H, Katsuyama E, Fujie A, Hao W, Kobayashi T, Sato Y, Miyamoto K, Morioka H, Matsumoto M, Chiba K, Toyama Y, Nakagawa T, Miyamoto T
  • Journal Title: Biochem Biophys Res Commun. Volume: 421(4) Pages: 785-9
  • Peer Reviewed
• [Journal Article] Aldehyde-stress resulting from Aldh2 mutation promotes osteoporosis due to impaired osteoblastogenesis (2012)
  • Author(s): Hoshi H, Hao W, Fujita Y, Funayama A, Miyauchi Y, Hashimoto K, Miyamoto K, Iwasaki R, Sato Y, Kobayashi T,Miyamoto H, Yoshida S, Mori T, Kanagawa H, Katsuyama E, Fujie A, Kitagawa K, Nakayama KI, Kawamoto T, Sano M, Fukuda K, Ohsawa I, Ohta S, Morioka H
  • Journal Title: J Bone Miner Res. Volume: 27(9) Pages: 2015-23
  • Peer Reviewed
• [Journal Article] Serum cartilage metabolites as biomarkers of degenerative lumbar scoliosis (2012)
  • Author(s): Hosogane N, Watanabe K, Tsuji T, Miyamoto T, Ishii K, Niki Y, Nakamura M, Toyama Y, Chiba K, Matsumoto M
  • Journal Title: J Orthop Res Volume: 30(8) Pages: 1249-53
  • Peer Reviewed
• [Journal Article] Osteoclast stimulatory transmembrane protein and dendritic cell-specific transmembrane protein cooperatively modulate cell-cell fusion to form osteoclasts and foreign body giant cells (2012)
  • Author(s): Miyamoto H, Suzuki T, Miyauchi Y, Iwasaki R, Kobayashi T, Sato Y, Miyamoto K, Hoshi H, Hashimoto K, Yoshida S, Hao W, Mori T, Kanagawa H, Katsuyama E, Fujie A, Morioka H, Matsumoto M, Chiba K, Takeya M, Toyama Y, Miyamoto T
  • Journal Title: J Bone Miner Res Volume: 27(6) Pages: 1289-97
  • Peer Reviewed
• [Journal Article] Risk factors for adjacent segment disease after posterior lumbar interbody fusion and efficacy of simultaneous decompression surgery for symptomatic adjacent segment disease (2012)
  • Author(s): Tomohiro Hikata
  • Journal Title: J spinal Disord Tech Volume: 印刷中
• [Presentation] 頚部脊柱管拡大術後の軸性疼痛に深層伸筋の末梢移動は関与するか (2013)
  • Author(s): 古川 満、鎌田 修博、蔵本 哲也
  • Organizer: JSSR
  • Place of Presentation: 沖縄
  • Year and Date: 2013-04-27
• [Presentation] 急速に進行した特発性脊髄血腫の1例 (2013)
  • Author(s): 大濱 玄、古川 満、蔵本 哲也、川崎 俊樹、千葉 和宏、鎌田 修博
  • Organizer: 関東整形災害外科
  • Place of Presentation: 栃木
• [Presentation] 頚部脊柱管拡大術後の軸性疼痛に深層伸筋の末梢移動は関与するか (2013)
  • Author(s): 古川 満
  • Organizer: 日本脊椎脊髄病学会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄）
• [Presentation] 急速に麻痺が進行した特発性脊髄出血の１例 (2013)
  • Author(s): 大濱 玄
  • Organizer: 関東整形災害外科
  • Place of Presentation: 宇都宮（栃木）
• [Presentation] プレガバリンは脊椎疾患の痺れを軽減させる新たな治療薬となりうるか (2012)
  • Author(s): 古川 満、鎌田 修博、蔵本 哲也、川崎俊樹、千葉 和宏
  • Organizer: 運動器疼痛学会
  • Place of Presentation: 東京
• [Presentation] プレガバリンは脊椎疾患の痺れを軽減させる新たな治療薬となるか (2012)
  • Author(s): 古川 満、鎌田 修博、蔵本 哲也、川崎俊樹、千葉 和宏
  • Organizer: 東日本整形災害外科
  • Place of Presentation: 群馬
• [Presentation] 白石式アプローチによる片開き式脊柱管拡大術の術後軸性疼痛減少には深層伸筋の抹消移動は関与するか (2012)
  • Author(s): 古川 満、鎌田 修博、蔵本 哲也
  • Organizer: JSTSS
  • Place of Presentation: 青森
• [Presentation] プレガバリンは脊椎疾患の痺れを軽減させる新たな治療薬となりうるか (2012)
  • Author(s): 古川 満
  • Organizer: 運動器疼痛学会
  • Place of Presentation: ＴＦＴビル(東京都)
• [Presentation] 白石式アプローチによる片開き式脊柱管拡大術の術後軸性疼痛減少には深層伸筋の末梢移動は関与するか (2012)
  • Author(s): 古川 満
  • Organizer: 日本脊椎脊髄手術手技
  • Place of Presentation: 青森
• [Presentation] プレガバリンは脊椎疾患の痺れを改善する新たな治療薬となるか？ (2012)
  • Author(s): 古川 満
  • Organizer: 東日本整形災害外科
  • Place of Presentation: 群馬