Investigation of articular cartilage degeneration and osteophyte formation in osteoarthritis using smoothened cond I t iona l knockout mice

• Project/Area Number: 22591695
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: NAKAMURA Eiichiro 産業医科大学, 医学部, 講師 (10412644)
• Co-Investigator(Renkei-kenkyūsha): NAKAMURA Toshitaka 産業医科大学, 医学部, 教授 (50082235) ; OTOMO Hajime 産業医科大学, 医学部, 助教 (40550372)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 骨・軟骨代謝学 / 変形性関節症 / 骨棘 / ヘッジホッグ / Cre-ER(T) / Hedgehog / Hedehog

Research Abstract

Osteophyte formation is a common phenomenon in osteoarthritis. Endochondral ossification is considered an important pathophysiological process for osteophyte fornlation. We investigated whether the deletion of smoothened (Smo), a key component of the hedgehog pathway in the process of enchondral ossification, inhibits osteophyte formation in nlouse osteoarthritis. The attenuation of osteophyte was shown in the mice deleted Smo, indicating that hedgehog signaling has a critical role in the osteoarthritis.

Research Products

• [Journal Article] Cre-mediated recombination can induce apoptosis in vivo by activating the p53 DNA damage-induced pathway (2012)
  • Author(s): Nakamura E, Zhu J, Nguyen MT, Yang J, Mackem S
  • Journal Title: Genesis Volume: 50(2) Pages: 102-111
  • Peer Reviewed
• [Journal Article] IGF-1R Signaling in chondrocytes modulates growth plate development by interacting with the PTHrP/Ihh pathway (2011)
  • Author(s): Wang Y, Cheng Z, ElAlieh HZ, Nakamura E, Nguyen MT, Mackem S, Clemens TL, Bikle DD, Chang W
  • Journal Title: JBone Miner Res Volume: 26(7) Pages: 1437-46
  • Peer Reviewed
• [Journal Article] IGF-1R Signaling in chondrocytes modulates growth plate development by interacting with the PTHrP/Ihh pathway (2011)
  • Author(s): Wang Y, Cheng Z, Elalieh HZ, Nakamura E, Nguyen MT, Mackem S, Clemens TL, Bikle DD, Chang W
  • Journal Title: J Bone Miner Res Volume: 26(7) Pages: 1437-1446
  • Peer Reviewed
• [Journal Article] IGF-IR Signaling in chondrocytes modulates growth plate development by interacting with the PTHrP/Ihh pathway. (2011)
  • Author(s): Wang Y, Cheng Z, Elalieh HZ, Nakamura E, Nguyen MT, Mackem S, Clemens TL, Bikle DD, Chang W.
  • Journal Title: J Bone Miner Res Volume: (Epub ahead of print)
  • Peer Reviewed
• [Presentation] 組織特異的遺伝子破壊マウスを利用した糖ヌクレオチド輸送体SLC35D1およびSLC35D2の軟骨分化・機能維持における機能解析 (2011)
  • Author(s): 平岡秀一、中村英一郎、古関明彦、浅原弘嗣
  • Organizer: 第24回日本軟骨代謝学会
  • Place of Presentation: 九州大学医学部百年講堂(福岡)
  • Year and Date: 2011-03-04