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The mechanism of general anesthesia investigated in the area of non-synaptic neural transmitting at the extracellular space in the brain: focusing on nitric oxide and dopaminergic system

Research Project

Project/Area Number 22591706
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionNagoya University (2011-2012)
Hamamatsu University School of Medicine (2010)

Principal Investigator

ADACHI Yushi  名古屋大学, 医学部附属病院, 病院講師 (80420355)

Co-Investigator(Kenkyū-buntansha) SATO Shigehito  浜松医科大学, 医学部, 教授 (30143176)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords非シナプス型細胞伝達 / 細胞外腔 / 一酸化窒素 / ドパミン / 麻酔作用 / ラット / 線条体 / マイクロダイアリシス / 麻酔作用機序 / 神経伝達 / 非シナプス性 / 全身麻酔 / マイクロダイアリシス法 / 麻酔薬 / ビキュキュリン / サクロフェン / プロポフォール / 相乗作用 / セボフルラン
Research Abstract

Pentobarbital (PB) and ketamine (Ket) influence the concentration of neurotransmitters in the brain. PB has been reported to decrease the extracellular nitric oxide (NO) concentration through a decrease in acetylcholine (ACh) release, while Ket has been shown to increase the NO concentration via an increase in ACh release. Here, we investigated effects of PB and Ket on NO release and the relationship between NO and ACh in the rat striatum by in vivo microdialysis experiments. Male Sprague-Dawley rats were used. A microdialysis probe was inserted into the right striatum and perfused with modified Ringer’s solution. Samples were collected every 15 min and injected into an HPLC system. The rats were freely moving, and PB and Ket were administered intraperitoneally. Neostigmine (1 and 10 μM) and mecamylamine (100 μM) were added to the perfusate. Calcium and magnesium concentrations were modified for each anesthetic to influence Ach release. PB decreased NO products (NOx) while Ket increase … More d them. While perfusion with neostigmine showed no effect on baseline Nox concentrations, it diminished the PB-inducedNOx reduction at low concentrations and abolished it at high concentrations. Magnesium-free perfusion had no effect on baseline Nox concentrations, whereas perfusion at a low magnesium concentration antagonized the PB-induced Nox reduction. Mecamylamine and calcium-free perfusion had no effect on baseline Nox concentrations and Ket-induced Nox increases. PB may decrease NO release through reduction in Ach release, whereas Ket may increase NO release independent of Ach regulation.Moreover, we studied a series of preliminary investigations and the results suggested that general anesthetics could modify many pathways of neural transmitting in extracellular space as a non-synaptic neural transmission. Not only GABA_A, which is one of well-known target receptors of anesthetics, but also GABA_Breceptor would be involved in as a target of general anesthetics with the current results of changing in releases of Nox. Less

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (41 results)

All 2013 2012 2011 2010 Other

All Journal Article (20 results) (of which Peer Reviewed: 18 results) Presentation (19 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Intravenous droperidol decreases the Bispectral index during general anesthesia with sevoflurane and remifentanil2013

    • Author(s)
      Adachi YU, Tanaka K, Suzuki S, Nishiwaki K, Matsuda N
    • Journal Title

      Masui

      Volume: 62 Pages: 71-4

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intravenous droperidol decreases the Bispectral index during general anesthesia with sevoflurane and remifentanil.2013

    • Author(s)
      Adachi YU, Tanaka K, Suzuki S, Nishiwaki K, Matsuda N.
    • Journal Title

      Masui

      Volume: 62 Pages: 71-4

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effect of aging on dopamine release and metabolism during sevoflurane anesthesia in rat striatum: An in vivo microdialysis study2012

    • Author(s)
      Kimura-Kuroiwa K, Adachi YU, Mimuro S, Obata Y, Kawamata M, Sato S, Matsuda N
    • Journal Title

      Brain Res Bull

      Volume: 89 Pages: 223-40

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dexmedetomidine and hydroxyzine synergistically potentiate the hypnotic activity of propofol in mice2012

    • Author(s)
      Kimura-Kuroiwa K, Adachi YU, Obata Y, Kawamata M, Sato S, Matsuda N
    • Journal Title

      J Anesth

      Volume: 26 Pages: 422-8

    • NAID

      10030827059

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pentobarbital decreased nitric oxide release in the rat striatum but ketamine increased the release independent of cholinergic regulation2012

    • Author(s)
      Kimura-Kuroiwa K, Adachi YU, Mimuro S, Kawamata M, Sato S, Matsuda N
    • Journal Title

      Exp Anim

      Volume: 61 Pages: 165-70

    • NAID

      10030555974

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cross-clamping of the descending thoracic aorta leads to the asymmetrical distribution of propofol during cardiopulmonary bypass surgery2012

    • Author(s)
      Yamauchi-Satomoto M, Adachi YU, Kurita T, Morita K, Sato S
    • Journal Title

      Korean J Anesthesiol

      Volume: 62 Pages: 327-31

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effect of aging on dopamine release and metabolism during sevoflurane anesthesia in rat striatum: An in vivo microdialysis study.2012

    • Author(s)
      Kimura-Kuroiwa K, Adachi YU, Mimuro S, Obata Y, Kawamata M, Sato S, Matsuda N.
    • Journal Title

      Brain Res Bull

      Volume: 89 Issue: 5-6 Pages: 223-40

    • DOI

      10.1016/j.brainresbull.2012.08.006

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dexmedetomidine and hydroxyzine synergistically potentiate the hypnotic activity of propofol in mice.2012

    • Author(s)
      Kimura-Kuroiwa K, Adachi YU, Obata Y, Kawamata M, Sato S, Matsuda N.
    • Journal Title

      J Anesth

      Volume: 26 Issue: 3 Pages: 422-8

    • DOI

      10.1007/s00540-012-1344-3

    • NAID

      10030827059

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cross-clamping of the descending thoracic aorta leads to the asymmetrical distribution of propofol during cardiopulmonary bypass surgery.2012

    • Author(s)
      Yamauchi-Satomoto M, Adachi YU, Kurita T, Morita K, Sato S.
    • Journal Title

      Korean J Anesthesiol

      Volume: 62 Issue: 4 Pages: 327-31

    • DOI

      10.4097/kjae.2012.62.4.327

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pentobarbital decreased nitric oxide release in the rat striatum but ketamine increased the release independent of cholinergic regulation.2012

    • Author(s)
      Kimura-Kuroiwa K, Adachi YU, Mimuro S, Kawamata M, Sato S, Matsuda N.
    • Journal Title

      Exp Anim

      Volume: 61 Pages: 165-70

    • NAID

      10030555974

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Is it constantly possible to penetrate only the anterior vessel wall against hydrostatic strain?2012

    • Author(s)
      Adachi YU, Tsuzuki M, Matsuda N
    • Journal Title

      Crit Care Med

      Volume: (印刷中)(in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Delayed recovery from general anesthesia in a patient with postpolio syndrome2012

    • Author(s)
      Adachi YU, Iwakiri S, Kimura T, Suzuki K, Obata Y
    • Journal Title

      Anesthesia and Resuscitation

      Volume: 48 Pages: 25-28

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epidural catheter insertion during general anesthesia2012

    • Author(s)
      Adachi YU, Matsuda N
    • Journal Title

      J Anesth

      Volume: (印刷中)(in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Is it fluid or air causing anesthesia mumps?2012

    • Author(s)
      Adachi YU, Matsuda N
    • Journal Title

      J Anesth

      Volume: (印刷中)(in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inflammatory alert cell strategy in septic acute lung injury2011

    • Author(s)
      Matsuda N, Tsuzuki M, Ichikawa S, Tochikubo J, Tamura T, Adachi Y
    • Journal Title

      Folia Pharmacologica Japonica

      Volume: 138 Issue: 4 Pages: 151-154

    • DOI

      10.1254/fpj.138.151

    • NAID

      10029767923

    • ISSN
      0015-5691, 1347-8397
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 敗血症性ショックにおけるステロイド使用の是非2011

    • Author(s)
      足立裕史
    • Journal Title

      ICUとCCU

      Volume: 35 Pages: 631-639

    • Related Report
      2011 Annual Research Report
  • [Journal Article] 炎症から見た敗血症の病態2011

    • Author(s)
      松田直之、田村哲也、杤久保順平、都築通孝、足立裕史
    • Journal Title

      Thrombosis Medicine

      Volume: 31 Pages: 143-150

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Four cases of inadvertent arterial cannulation despite of ultrasound guidance2010

    • Author(s)
      Adachi YU, Sato S.
    • Journal Title

      Am J Emerg Med

      Volume: 28 Pages: 28-29

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deterioration of myocardial injury due to dexmedetomidine administration after myocardial ischaemia.2010

    • Author(s)
      Mimuro S, Katoh T, Suzuki A, Yu S, Adachi YU, Uraoka M, Sano H, Sato S.
    • Journal Title

      Resuscitation

      Volume: 81 Pages: 1714-1717

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A case of unexpected cervical tuberculous lymphadenitis detected during ultrasoud-guided central venous catheter insertion.2010

    • Author(s)
      Itagaki T, Mimuro S, Kawashima S, Adachi Y, Doi M, Sato S.
    • Journal Title

      Anesthesia and Resuscitation

      Volume: 46 Pages: 63-64

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Co-Administration of JM-1232(-) and Propofol Produced Quick Emergence From Hypnosis and the Repeated Injections Demonstrated Minute Increases of Recovery Time From Anesthesia in ddY Mice2012

    • Author(s)
      Adachi Y, Tochikubo J, Tamura T, Matsuda N
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2012, A546
    • Place of Presentation
      Washington D.C. USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Droperidol, But Not Haloperidol, Enhanced the Hypnotic Activity of Propofol and Co-administration of Haloperidol With Quinelorane Showed a Potentiation of Propofol-induced Hypnosis in ddY Mice2012

    • Author(s)
      Adachi Y, Tochikubo J, Tamura T, Matsuda N.
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2012, A740
    • Place of Presentation
      Washington D.C. USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Droperidol Decreases the Bispectral Index During General Anesthesia With Sevoflurane and Remifentanil2012

    • Author(s)
      Adachi Y, Tochikubo J, Tamura T, Matsuda N
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2012, A1196
    • Place of Presentation
      Washington D.C. USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Co-administration of JM-1232(-) synergistically enhanced the hypnotic activity of propofol and the combination shortened the recovery from anesthesia in ddY mice2012

    • Author(s)
      Adachi YU
    • Organizer
      Euroanaesthesia 2012, 9AP2-4
    • Place of Presentation
      Paris, France
    • Related Report
      2012 Final Research Report
  • [Presentation] Co-administration of JM-1232(-) synergistically enhanced the hypnotic activity of propofol and the combination shortened the recovery from anesthesia in ddY mice.2012

    • Author(s)
      Adachi YU.
    • Organizer
      Euroanaesthesia 2012
    • Place of Presentation
      Paris, France
    • Related Report
      2012 Annual Research Report
  • [Presentation] Co-Administration of JM-1232(-) and Propofol Produced Quick Emergence From Hypnosis and the Repeated Injections Demonstrated Minute Increases of Recovery Time From Anesthesia in ddY Mice2012

    • Author(s)
      Adachi Y.
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2012
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Droperidol, But Not Haloperidol, Enhanced the Hypnotic Activity of Propofol and Co-administration of Haloperidol With Quinelorane Showed a Potentiation of Propofol-induced Hypnosis in ddY Mice2012

    • Author(s)
      Adachi Y.
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2012
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] Droperidol Decreases the Bispectral Index During General Anesthesia With Sevoflurane and Remifentanil.2012

    • Author(s)
      Adachi Y.
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2012
    • Place of Presentation
      Washington DC, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] GABA(B) receptor antagonist, saclofen, reduced nitric oxide release in the rat striatum, whereas, abolished the sevoflurane-induced nitric oxide release, using in vivo microdialysis experiment2011

    • Author(s)
      Adachi YU, Matsuda N
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2011, A323
    • Place of Presentation
      Chicago, IL, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] JM-1232(-) synergistically potentiated the hypnotic activity of propofol without delayed recovery from anesthesia in ddY mice2011

    • Author(s)
      Adachi YU, Matsuda N
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2011, A1211
    • Place of Presentation
      Chicago, IL, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Dexmedetomidine and hydroxyzine synergistically potentiated the hynotic activity of propofol in ddY mice.2011

    • Author(s)
      Adachi YU, Matsuda N
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2011, A1588
    • Place of Presentation
      Chicago, IL, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Large dose haloperidol decreased the extracellular concentration of nitric oxide products and propofol did not modify the change in the rat brain striatum: in vivo microdialysis experiment2011

    • Author(s)
      Adachi YU, Takada K, Sato S, Matsuda N
    • Organizer
      Euroanaesthesia 2011, 7AP4-7
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2012 Final Research Report
  • [Presentation] The propofol-induced inhibition of nitric oxide release in rats brain was antagonized by perfusion of saclofen, GABAA receptor antagonist, using in vivo microsdialysis experiment2011

    • Author(s)
      Adachi YU, Kimura-Kuroiwa K, Kobayashi A, Sato S, Matsuda N
    • Organizer
      Euroanaesthesia 2011, 7AP4-8
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2012 Final Research Report
  • [Presentation] Large dose haloperidol decreased the extracellular concentration of nitric oxide products and propofol did not modify the change in the rat brain striatum : in vivo microdialysis experiment2011

    • Author(s)
      Adachi YU, Takada K, Sato S, Matsuda N
    • Organizer
      Euroanaesthesia 2011
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2011 Annual Research Report
  • [Presentation] The propofol-induced inhibition of nitric oxide release in rats brain was antagonized by perfusion of saclofen, GABAA receptor antagonist, using in vivo microsdialysis experiment2011

    • Author(s)
      Adachi YU, Kimura-Kuroiwa K, Kobayashi A, Sato S, Matsuda N
    • Organizer
      Euroanaesthesia 2011
    • Place of Presentation
      Amsterdam, The Netherlands
    • Related Report
      2011 Annual Research Report
  • [Presentation] GABA(B) receptor antagonist, saclofen, reduced nitric oxide release in the rat striatum, whereas, abolished the sevoflurane-induced nitric oxide release, using in vivo microdialysis experiment2011

    • Author(s)
      Adachi YU, Matsuda N
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2011
    • Place of Presentation
      Chicago, IL, USA
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dexmedetomidine and hydroxyzine synergistically potentiated the hynotic activity of propofol in ddY mice2011

    • Author(s)
      Adachi YU, Takada K, Sato S, Matsuda N
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2011
    • Place of Presentation
      Chicago, IL, USA
    • Related Report
      2011 Annual Research Report
  • [Presentation] JM-1232(-) synergistically potentiated the hypnotic activity of propofol without delayed recovery from anesthesia in ddY mice2011

    • Author(s)
      Adachi YU, Takada K, Sato S, Matsuda N
    • Organizer
      Annual meeting of American Society of Anesthesiologists 2011
    • Place of Presentation
      Chicago, IL, USA
    • Related Report
      2011 Annual Research Report
  • [Presentation] The nitroglycerine-induced nitric oxide release was enhanced by propofol and midazolam not by sevoflurane in rats.2010

    • Author(s)
      Adachi YU, Kimura S, Mimuro S, Obata Y, Sato S.
    • Organizer
      Euroanaesthesia 2010
    • Place of Presentation
      Helsinki, Finland
    • Related Report
      2010 Annual Research Report
  • [Book] ER・ICUでの薬の使い方Q&A-プロの実践と秘訣に学ぶ-2010

    • Author(s)
      足立裕史, ed by岡元和文
    • Publisher
      総合医学社
    • Related Report
      2010 Annual Research Report
  • [Remarks] 名古屋大学

    • URL

      http://www.nagoya-u.ac.jp/

    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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