| Project/Area Number |
22591712
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Yokohama City University |
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Principal Investigator |
ANDOH Tomio 横浜市立大学, 医学研究科, 客員教授 (00193110)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIYA Yoshinori 横浜市立大学, 医学研究科, 客員講師 (90381491)
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| Co-Investigator(Renkei-kenkyūsha) |
ECHIGO Noriyuki 横浜市立大学, 医学研究科, 客員准教授 (00363797)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ブメタニド / NKCC1 / pCREB / Ca イメージング / 鎮静作用 / GABAA受容体 / 新生児 / 麻酔薬 / ミダゾラム / 幼若脳 / 細胞内Ca濃度 / GABA_A受容体 |
|---|
| Research Abstract |
We intended to test the hypothesis that midazolam may exert excitatory actions on the developing hippocampus and may reduce sedative effects in neonatal rats. We found that midazolam increased intracellular Ca concentration and p CREB expression in the hippocampal CA3 area from neonatal rats but not from young rats. These excitatory effects of midazolam were inhibited by bumetanide, an inhibitor for NKCC1. Midazolam exhibited less sedative actions in neonatal rats compared with in young rats. Bumetanide enhanced sedative effects of midazolam in neonatal but not young rats. These results indicate that GABAA receptor-mediated excitation is dependent on NKCC1 and may underlie the attenuated sedative effects of midazolam in neonatal rats.
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