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Effects of general anesthetics on the neural activity of the immature nervous system.

Research Project

Project/Area Number 22591712
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Anesthesiology/Resuscitation studies
Research InstitutionYokohama City University

Principal Investigator

ANDOH Tomio  横浜市立大学, 医学研究科, 客員教授 (00193110)

Co-Investigator(Kenkyū-buntansha) KAMIYA Yoshinori  横浜市立大学, 医学研究科, 客員講師 (90381491)
Co-Investigator(Renkei-kenkyūsha) ECHIGO Noriyuki  横浜市立大学, 医学研究科, 客員准教授 (00363797)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsブメタニド / NKCC1 / pCREB / Ca イメージング / 鎮静作用 / GABAA受容体 / 新生児 / 麻酔薬 / ミダゾラム / 幼若脳 / 細胞内Ca濃度 / GABA_A受容体
Research Abstract

We intended to test the hypothesis that midazolam may exert excitatory actions on the developing hippocampus and may reduce sedative effects in neonatal rats. We found that midazolam increased intracellular Ca concentration and p CREB expression in the hippocampal CA3 area from neonatal rats but not from young rats. These excitatory effects of midazolam were inhibited by bumetanide, an inhibitor for NKCC1. Midazolam exhibited less sedative actions in neonatal rats compared with in young rats. Bumetanide enhanced sedative effects of midazolam in neonatal but not young rats. These results indicate that GABAA receptor-mediated excitation is dependent on NKCC1 and may underlie the attenuated sedative effects of midazolam in neonatal rats.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (7 results)

All 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (6 results)

  • [Journal Article] Bumetanide, an inhibitor of cation-chloride co-transporter isoform 1, inhibits GABAergic excitatory actions and enhances sedative actions of midazolam in neonatal rats.

    • Author(s)
      Yukihide Koyama, Tomio Andoh, Yoshinori Kamiya, Satoshi Morita, Tomoyuki Miyazaki, Kazuhiro Uchimoto, Takahiro Mihara, Takahisa Goto
    • Journal Title

      Anesthesiology

      Volume: (in press)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] the inhibitor of NKCC1, cation-chloride co-transporter isoform 1, inhibits the GABAergic excitatory action induced by midazolam in the hippocampus in neonatal rats.2013

    • Author(s)
      Yukihide Koyama, Tomio Andoh, Yoshinori Kamiya, Takahisa GotoBumetanide
    • Organizer
      Euroanaesthesia 2013
    • Place of Presentation
      Barcelona, Spain
    • Related Report
      2012 Final Research Report
  • [Presentation] Na+, K+-2Cl- co-transporter (NKCC1) の選択的阻害薬のブメタニドは新生児ラットの海馬 CA3 領域における GABAA 受容体活性化による興奮性活動を抑制する2013

    • Author(s)
      小山 行秀、安藤 富男、紙谷 義孝、後藤 隆久
    • Organizer
      日本麻酔科学会総会
    • Place of Presentation
      ロイトン札幌他(北海道)
    • Related Report
      2012 Final Research Report
  • [Presentation] Bumetanide, the inhibitor of NKCC1 (Cation-Chloride Co-Transporter Isoform 1), inhibited an increase in cytosolic Ca2+ concentration ([Ca2+]i) induced by midazolam in the immature hippocampus2013

    • Author(s)
      Yukihide Koyama, Tomio Andoh, Yoshinori Kamiya, Takahisa Goto
    • Organizer
      World Anesthesia Convention
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2012 Final Research Report
  • [Presentation] BUMETANIDE, THE INHIBITOR OF NKCC1, CATION-CHLORIDE CO-TRANSPORTER ISOFORM 1, INHIBITED AN INCREASE IN CYTOSOLIC CA2+ CONCENTRATION [CA2+]I INDUCED BY MIDAZOLAM IN IMMATURE HIPPOCAMPUS.2013

    • Author(s)
      小山行秀、安藤富男
    • Organizer
      4th World Anesthesia Convention 2013
    • Place of Presentation
      Bangkok, Tailand
    • Related Report
      2012 Annual Research Report
  • [Presentation] Na+, K+-2Cl- co-transporter (NKCC1)の選択的阻害薬のブメタニドは新生児ラットの海馬CA3領域におけるGABAA受容体活性化による興奮性活動を抑制する2013

    • Author(s)
      小山行秀、安藤富男
    • Organizer
      第60回日本麻酔科学会学術総会
    • Place of Presentation
      ロイトン札幌他、北海道
    • Related Report
      2012 Annual Research Report
  • [Presentation] Bumetanide, the inhibitor of NKCC1, cation-chloride co-transporter isoform 1, inhibits the GABAergic excitatory action induced by midazolam in the hippocampus in neonatal rats.2013

    • Author(s)
      小山行秀、安藤富男
    • Organizer
      Euroanaesthesia 2013
    • Place of Presentation
      Barcelona, Spain
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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