| Project/Area Number |
22591717
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
|
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| Research Institution | Showa University (2011-2012)
Kyorin University (2010) |
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Principal Investigator |
|
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Akiko 昭和大学, 歯学部, 助教 (00551227)
|
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | FRET / apoptosis / YFP / CFP / venus / neuronal death / caspase8 / propofol / Bid / anesthetics / アポトーシス / 麻酔薬 / カスパーゼ / 神経細胞 / 初代培養 / Venus / ミトコンドリア / 膜電位 / 脳保護 |
|---|
| Research Abstract |
Apoptosis is deeply related to neuronal injury. Apoptosis-related proteins are incrementally cleaved, and it would be a good tool for the research of neuronal injury to catch this initial step of the apoptotic cascades. We applied the technique of FRET(Fluorescence of resonance energy transfer) to the neuronal death model of primary neuronal culture, and tried to make a resolutional image of cleavage of apoptosis-related protein. We have developed the protocol of quantitative analysis of FRET change of apoptosis-induced neuronal culture.
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