A new approach to explore the mechanism of anesthesia-induced unconsciousness. - The role of the hypothalamic MCH neurons -

• Project/Area Number: 22591718
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Teikyo University
• Principal Investigator: FUKUDA Satoru 帝京大学, 医学部, 教授 (30116751)
• Co-Investigator(Renkei-kenkyūsha): SAKAMOTO Hidetoshi 帝京大学, 医学部, 助教 (90349267) ; MORITA Shigeho 帝京大学, 医学部, 教授 (60143476) ; SAWAI Jyun 帝京大学, 医学部, 助手 (20527410) ; TAKATA Jyunko 帝京大学, 国際教育研究所, 助手 (00527418) ; SENTHO Yoshiki 帝京大学, 国際教育研究所, 助手 (80527416)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: メラニン凝集ホルモン / 睡眠・覚醒 / 記憶 / 麻酔 / Neuropeptide EI / 脳室内投与 / 海馬 / REM睡眠 / ｱｾﾁﾙｺﾘﾝ / マイクロダイアリシス / アセチルコリン / 脳波 / 筋電図 / 麻酔機序 / ラット / オレキシン

Research Abstract

We investigated the effects of melanin concentrating hormone (MCH) on sleep-wake cycle. Intraventricular (icv) injection of MCH decreased the waking episode time, whereas it increased the sleep episode time (REM & nonREM episode time). It has been reported that sleep is necessary for consolidation of memory with increasing the hippocampal acetylcholine (ACh). In the present study, icv injection of MCH increased the hippocampal ACh. There was a significant correlation between REM episode time and hippocampal ACh effluxes. Icv injection of MCH did not alter the cortical histamine release. These findings suggest that MCH may contribute to the consolidation of memory. Further experiment is necessary to explore whether the memory function of MCH may be involved in the anesthetic state.

Research Products

• [Journal Article] Melanin concentrating hormone induces hippocampal acetylcholine release via the medial septum in rats (2013)
  • Author(s): Zhi-hong Lu, Satoru Fukuda, Yoichi Minakawa, Atsushi Yasuda, Hidetoshi Sakamoto, Shigehito Sawamura, Hidenori Takahashia, Noriko Ishii
  • Journal Title: Peptides Volume: 44 Pages: 32-29
• [Journal Article] Melanin concentrating hormone induces hippocampal acetylcholine release via the medial septum in rats (2013)
  • Author(s): Zhi-hong Lu
  • Journal Title: Peptides Volume: 44 Pages: 32-39
  • Peer Reviewed
• [Presentation] メラニン凝集ホルモンの記憶促進作用 (2012)
  • Author(s): 皆川 陽一、路 志紅、福田 悟、坂本 英俊、澤村 成史、高橋 秀則
  • Organizer: 日本麻酔科学会第59回学術集会
  • Place of Presentation: 神戸
• [Presentation] Effects of intraventricular melanin-concentrating hormone (MCH) microinjections on sleep and acetylcholine release (2011)
  • Author(s): 路志紅
  • Organizer: 日本麻酔科学会第58回学術大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-06-19
• [Presentation] Effects of intraventricular melanin-concentrating hormone (MCH) microinjections on sleep and cortical acetylcholine release (2011)
  • Author(s): Zhi-hong Lu, Satoru Fukuda, AtsushiYasuda, Hidetoshi Sakamoto, Shigeho Morita
  • Organizer: 日本麻酔科学会第58回学術集会
  • Place of Presentation: 神戸
• [Presentation] メラニン凝集ホルモンの記憶促進作用
  • Author(s): 皆川 陽一
  • Organizer: 日本麻酔科学会第59回学術大会
  • Place of Presentation: 神戸