The analysis and treatment of immuno-suppressive state in cellular immunity induced by sepsis

• Project/Area Number: 22591728
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology/Resuscitation studies
• Research Institution: Osaka University
• Principal Investigator: OHTA Noriyuki 大阪大学, 医学部附属病院, 助教 (60379162)
• Co-Investigator(Kenkyū-buntansha): FUJINO Yuji 大阪大学, 大学院・医学系研究科, 教授 (50252672) ; GOTO Yukiko 大阪大学, 大学院・医学系研究科, 助教 (20276710)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 敗血症 / 免疫応答 / 細胞傷害性リンパ球 / 粘膜免疫 / サイトカイン / PD-L1 / コレラ毒素 / 細胞性免疫 / マウス / 腹膜炎 / 鎮静薬 / 樹状細胞 / 腫瘍

Research Abstract

We analyzed the immunosuppressive state after recovery from sepsis induced by peritonitis in mice. We mainly used experimental system analyzing antigen-specific immune-response induced by mucosal immunization method. In mice recovered from sepsis, antigen-specific immune-response was depressed in terms of cellular immunity. Furthermore, we tried the improvement of depressed immune-response by intervention into PD-1 and PD-L1 signalling.

Research Products

• [Journal Article] The comparison of spontaneous breathing and muscle paralysis in two different severities of experimental lung injury (2013)
  • Author(s): Yoshida T, Uchiyama A, Matsuura N, Mashimo T, Fujino Y.
  • Journal Title: Crit Care Med Volume: 41 Pages: 536-45
  • Peer Reviewed
• [Journal Article] Spontaneous breathing during lung-protective ventilation in an experimental acute lung injury model: high transpulmonary pressure associated with strong spontaneous breathing effort may worsen lung injury. (2012)
  • Author(s): Yoshida T, Uchiyama A, Matsuura N, Mashimo T, Fujino Y
  • Journal Title: Crit Care Med. Volume: 40 Pages: 1578-85
  • Peer Reviewed
• [Journal Article] Midazolam suppresses maturation of murine dendritic cells and priming of lipopolysaccharide-induced t helper 1-type immune response. (2011)
  • Author(s): Ohta N, Ohashi Y, Takayama C, Mashimo T, Fujino Y.
  • Journal Title: Anesthesiology. Volume: 114 Pages: 355-62
  • Peer Reviewed
• [Journal Article] The effect of ventilator performance on airway pressure release ventilation: a model lung study (2011)
  • Author(s): Yoshida T, Uchiyama A, Mashimo T, Fujino Y.
  • Journal Title: Anesth Analg Volume: 113 Pages: 529-33
  • Peer Reviewed
• [Journal Article] Midazolam suppresses maturation of murine dendritic cells and priming of lipopolysaccharide-induced T helper-1 type immune response (2011)
  • Author(s): 大田典之, 藤野裕士
  • Journal Title: Anesthesiology Volume: 114 Pages: 355-362
  • Peer Reviewed
• [Journal Article] The effect of ventilator performance on airway pressure release ventilation : alung model study (2011)
  • Author(s): 藤野裕士
  • Journal Title: Anesthesia and Analgesia Volume: 113 Pages: 529-533
  • Peer Reviewed
• [Journal Article] Midazolam suppresses maturation of murine dendritic cells and priming of lipopolysaccharide-induced t helper 1-type immune response (2011)
  • Author(s): 大田典之、藤野裕士
  • Journal Title: Anesthesiology. Volume: 114 Pages: 355-62
  • Peer Reviewed
• [Journal Article] Propofol has anti-inflammatory effects on alveolar type II epithelial cells (2010)
  • Author(s): 馬鈴、藤野裕士
  • Journal Title: Acta Anaesthesiol Scand. Volume: 54 Pages: 362-9
  • Peer Reviewed
• [Journal Article] Evaluation of performance of two high-frequency oscillatory ventilators using a model lung with a position sensor. (2010)
  • Author(s): 井口直也、藤野裕士
  • Journal Title: J Anesth Volume: 24 Pages: 888-92
  • NAID: 10027741404
  • Peer Reviewed
• [Journal Article] Effects of continuous venovenous hemofiltration on the pharmacology of carperitide, a recombinant-human atrial natriuretic peptide (2010)
  • Author(s): 高橋りょう子、藤野裕士
  • Journal Title: Circ J Volume: 74 Pages: 1888-92
  • NAID: 10026971432
  • Peer Reviewed
• [Journal Article] Spontaneous breathing during lung protective ventilation in experimental acute lung injury model : high transpulmonary pressure associated with strong spontaneous breathing effort may worsen lung injury
  • Author(s): 藤野裕士
  • Journal Title: Critical Care Medicine Volume: (掲載確定)
  • Peer Reviewed
• [Presentation] ミダゾラムは樹状細胞による抗原特異的な免疫応答を抑制する (2013)
  • Author(s): 大田典之
  • Organizer: 第40回日本集中治療医学会総会
  • Place of Presentation: 松本
  • Year and Date: 2013-03-02
• [Presentation] ミダゾラムは樹状細胞による抗原特異的な応答を抑制する (2013)
  • Author(s): 大田典之
  • Organizer: 第４０回日本集中治療医学会学術総会
  • Place of Presentation: 松本
• [Presentation] プロポフォールは樹状細胞によって誘導される Th1 型免疫応答を促進する (2012)
  • Author(s): 大田典之
  • Organizer: 第59回日本麻酔科学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2012-06-08
• [Presentation] 敗血症は粘膜免疫応答を低下させる (2012)
  • Author(s): 大田典之
  • Organizer: 第39回日本集中治療医学会総会
  • Place of Presentation: 千葉
  • Year and Date: 2012-03-01
• [Presentation] 敗血症は粘膜免疫応答を低下させる (2012)
  • Author(s): 大田典之, 藤野裕士
  • Organizer: 日本集中治療医学会総会
  • Place of Presentation: 幕張(千葉)(招待講演)
  • Year and Date: 2012-03-01
• [Presentation] プロポフォールは樹状細胞によって惹起されるTh1型免疫応答を増強する (2012)
  • Author(s): 大田典之, 藤野裕士
  • Organizer: 日本集中治療医学会総会
  • Place of Presentation: 幕張(千葉)(招待講演)
  • Year and Date: 2012-03-01
• [Presentation] プロポフォールは樹状細胞によって誘導されるTh1型免疫応答を促進する (2012)
  • Author(s): 大田典之
  • Organizer: 第５９回日本麻酔科学会学術集会
  • Place of Presentation: 神戸
• [Presentation] 樹状細胞の機能に対する鎮静薬の機能の多様性 (2011)
  • Author(s): 大田典之
  • Organizer: 第38回日本集中治療医学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2011-02-26
• [Presentation] ミダゾラムは樹状細胞によって惹起されるTh1 型の免疫応答を抑制する (2010)
  • Author(s): 大田典之
  • Organizer: 第57回日本麻酔科学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2010-06-03
• [Presentation] ミダゾラムは樹状細胞によって惹起されるTh1型の免疫応答を抑制する (2010)
  • Author(s): 大田典之
  • Organizer: 日本麻酔科学会第57回学術集会
  • Place of Presentation: 福岡(招待講演)
  • Year and Date: 2010-06-03
• [Presentation] ミダゾラムは自己非自己の認識能を低下させる (2010)
  • Author(s): 大田典之
  • Organizer: 第60回日本麻酔科学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2010-05-23