| Project/Area Number |
22591753
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SOEN Masako 大阪医科大学, 医学部, 助教 (90445982)
FUJIWARA Shunsuke 大阪医科大学, 医学部, 助教 (60535813)
MORIMOTO Kenji 大阪医科大学, 医学部, 助教 (20388250)
MIYAZAKI Shinichiro 大阪医科大学, 医学部, 助教 (30411359)
KUSAKA Yusuke 大阪医科大学, 医学部, 助教 (60636075)
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| Co-Investigator(Renkei-kenkyūsha) |
FURUTA Kyoji 岐阜大学, 大学院・医学系研究科再生医科学専攻・医学(系)研究科(研究院), 准教授 (40173538)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | アクロメリン酸 / アロディニア / 神経障害性疼痛 / カイニン酸 / 脊髄 |
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| Research Abstract |
We previously showed that intrathecal (i.t.) administration ofacromelic acid A (Acro-A), a major causing substance of the mushroom (Clitocybeacromelalga) poison, provoked allodynia in conscious mice. To clarify a mechanism ofAcro-A, we synthesized [11C]-labeled Acro-A analogue (PSPA-4) that can competitivelyblock the Acro-A-induced allodynia. In vitro autoradiography, [C]PSPA-4 wasspecifically bound to the rat brain and spinal cord, and the binding was significantlydisplaced by kainic acid, but not by antagonists of kainate receptor. PSPA-4 attenuatedthe Acro-A-induced allodynia at low doses and induced allodynia at high doses via a bindingsite different from known kainate antagonists. The development of a radio-labeled Acro-Aanalogue will enable us to promote the understanding of the action mechanism not onlyof Acro-A, but also of pain transmission in the periphery and central nervous system.
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