Regulation of oncogenes or tumor suppressor genes in Urological cancer by micro RNA

• Project/Area Number: 22591759
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Yamagata University
• Principal Investigator: TOMITA Yoshihiko 山形大学, 医学部, 教授 (90237123)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: マイクロRNA / 泌尿器科癌 / 遺伝子

Research Abstract

Enforced expressions of miR-199a and miR-101, of which expressions are inhibited in renal cell cancer (RCC), leads to suppression of GSK3β and EZH2 resulting in reducing cancer cell proliferation. Re-introduction of miR-199a and/or miR-101 might be a beneficial treatment strategy. Reduced expression of ATBF1 is correlated with more malignant character of RCC. hsa-miR-203, hsa-miR-194, hsa-miR-204 are proposed as suppressors of ATBF1 might be novel targets for another new treatment modality.

Research Products

• [Journal Article] The enhancer of zeste homolog 2 (EZH2), a potential therapeutic target, is regulated by miR-101 in renal cancer cells (2012)
  • Author(s): Sakurai T, Bilim VN, Ugolkov AV, YuukiK, Tsukigi M, Motoyama T, Tomita Y
  • Journal Title: Biochem Biophys Res Commun Volume: 422 Issue: 4 Pages: 607-614
  • DOI: 10.1016/j.bbrc.2012.05.035
  • Peer Reviewed
• [Journal Article] Re-expression of miR-199a suppresses renal cancer cell proliferation and survival by targetingGSK-3β (2012)
  • Author(s): Tsukigi M,Bilim V,Yuuki K,Ugolkov A,Naito S,Nagaoka A,Kato T,Motoyama T, Tomita Y
  • Journal Title: Cancer Letters Volume: 315 Issue: 2 Pages: 189-197
  • DOI: 10.1016/j.canlet.2011.10.008
  • Peer Reviewed
• [Presentation] 腎細胞癌におけるmiR-199aの低発現は、GSK-3βを上昇させ、癌増殖に働く (2011)
  • Author(s): 槻木真明,内藤整,ビリームウラジミル,結城加織,川添久,櫻井俊彦,細谷法之,本山悌一,冨田善彦
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-04
• [Presentation] 腎細胞癌におけるmiR-199aの低発現は、GSK3βを上昇させ、癌増殖に働く (2011)
  • Author(s): 槻木真明
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋市(名古屋国際会議場)
  • Year and Date: 2011-10-04
• [Presentation] Yoshihiko Tomita Low miR-199a expression in Renal Cell Carcinoma (RCC) is related to malignant phenotype via upregulation of GSK-3 β (2011)
  • Author(s): Masaaki Tsukigi, Vladimir Bilim, Kaori Yuuki, Andrey Ugolkov, Sei Naito,Hisashi Kawazoe, Toshihiko Sakurai, Noriaki Hoshoya, Narisawa Takahumi, Akira Nagaoka, Tomoyuki Kato, Mototsugu Oya, Teiichi Motoyama
  • Organizer: AUA. AnnualMeeting, Washington D.C, USA Walter E.
  • Place of Presentation: Washington Convention Center
  • Year and Date: 2011-05-15
• [Presentation] mir199-5pは腎細胞癌(clear cell carcinoma)において GSK3βを抑制する (2011)
  • Author(s): 槻木真明,内藤整,結城加織,ビリームウラジミル,川添久,櫻井俊彦,細谷法之,成澤貴史,黒田悠太,菅野秀典,西田隼人,真島英太,冨田善彦
  • Organizer: 第99回日本泌尿器科学会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-04-24
• [Presentation] mir199a-5pは腎細胞癌(clear cell carcinoma)においてGSK3βを抑制する (2011)
  • Author(s): 槻木真明
  • Organizer: 第99回日本泌尿器科学会総会
  • Place of Presentation: 名古屋市(名古屋国際会議場)
  • Year and Date: 2011-04-24
• [Presentation] 腎細胞癌におけるEnhancer of zeste homolog 2の発現上昇は,microRNA101の発現低下が関与している (2010)
  • Author(s): 櫻井俊彦,ビリームウラジミル,結城加織,槻木真明,冨田善彦
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-09-24
• [Presentation] Expression of enhancer of zeste homolog 2 in renal cell carcinoma and relationship todowanregulation of micro RNA-101 (2010)
  • Author(s): T.Sakurai, V.Bilim, K.Yuuki, M.Tsukigi,A.V.Ougolkov, Y.Yomita
  • Organizer: ASCO, Chicago
  • Place of Presentation: USA MCCORMICK PLACE
  • Year and Date: 2010-06-07
• [Presentation] ENHANCER OF ZESTE HOMOLOG 2 IS OVEREXPRESSED IN RENAL CELL CARCINOMA POSSIBLY DUE TO DOWNREGULATION OF MICRORNA-101 (2010)
  • Author(s): Toshihiko Sakurai, Vladimir Bilim, Kaori Yuuki, Masaaki Tsukigi, Yoshihiko Tomita
  • Organizer: AUA Annual Meeting,san francisco
  • Place of Presentation: USA Moscone ConventionCenter
  • Year and Date: 2010-05-30