Project/Area Number |
22591764
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Urology
|
Research Institution | Mie University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
HASEGAWA Yoshihiro 三重大学, 医学部附属病院, 助教 (10402687)
ISHII Kenichiro 三重大学, 大学院・医学系研究科, 助教 (90397513)
木瀬 英明 三重大学, 医学部附属病院, 講師 (80293786)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 細胞外マトリックス / 前立腺 / 膀胱 / テネイシンC / 泌尿生殖洞 / 二核細胞 / GeneChip解析 / 遺伝子欠損マウス / 胎児泌尿生殖洞 / チモシンβ10 / 形態形成 / 間質-上皮の相互作用 / 組織組み替え実験 |
Research Abstract |
Morphogenesis in tissue development depends on the relationships among cells, the extracellular matrix (ECM), and other support structures. Tenascin-C (TN-C) is a hexametric glycoprotein of the ECM produced mainly by stromal cells. In this study, we evaluated the gene expression profiles in TN-C knockout (TN-C KO) mouse urogenital sinus mesenchyme (mUGM). In TN-C KO mUGM, the expressions of thymosinβ10 and specific genes related to chemotaxis, cell-matrix adhesion, response to wounding, and collagen fibril organization were decreased or disappeared as compared to those in WT mUGM, suggesting that stromal TN-C signals and/or related signals might play an important role in development and organogenesis of prostate.
|