Development of new diagnostic methods for bladder cancer using GFP expressing adenovirus vector

• Project/Area Number: 22591767
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Urology
• Research Institution: Okayama University
• Principal Investigator: KAKU Haruki 岡山大学, 岡山大学病院, 講師 (60346426)
• Co-Investigator(Kenkyū-buntansha): WATANABE Masami 岡山大学, 岡山大学病院, 准教授 (70444677)
• Co-Investigator(Renkei-kenkyūsha): HUJIWARA Toshiyuki 岡山大学, 医歯薬学総合研究科, 教授 (00304303) ; KAGAWA Shunnsuke 岡山大学, 岡山大学病院, 講師 (00362971)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 緑色蛍光タンパク質 / ウイルスベクター / 膀胱癌 / プラスミド / 癌診断 / 蛍光蛋白（GFP） / 遺伝子発現プラスミド / ウイルス製剤 / GFP発現プラスミド試薬 / 診断法

Research Abstract

Usefulness of a diagnostic novel adenoviralvector Telomescan, which proliferates specifically in cancer cells and expresses green fluorescent protein (GFP), in the detection of various bladder cancer cells, was demonstrated. In addition, the usefulness of the luciferase and GFP expression plasmids (hTERT-AdTSTA-luciferase and GFP) as next-generation cancer-specific expression systems was also demonstrated in a various types of bladder cancer cells. Using these tools, we expect that detection of disseminating cancer cells becomes possible in a wide range of cancer diseases.

Research Products

• [Journal Article] In vivo imaging of transplanted islets labeled with a novel cationic nanoparticle (2013)
  • Author(s): Oishi K, Miyamoto Y, Saito H, Murase K, Ono K, Sawada M, Watanabe M, Noguchi Y, Fujiwara T, Hayashi S, Noguchi H
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Clinical significance of CD24 as a predictor of bladder cancer recurrence (2013)
  • Author(s): Chunxiao Liu, Shaobo Zheng, Haiyan Shen, Kai Xu, Jie Chen, Hulin Li, Yawen Xu, Abai Xu, Binshen Chen, Haruki Kaku, Yasutomo Nasu, Hiromi Kumon, Peng Huang, Masami Watanabe
  • Journal Title: Oncology letters
  • Peer Reviewed
• [Journal Article] Cancer stem cell-likecharacteristics of a CD133+ subpopulation in the J82 human bladder cancer cell line (2013)
  • Author(s): Peng Huang, Masami Watanabe,Haruki Kaku
  • Journal Title: Molecular and Clinical Oncology Volume: 1 Pages: 180-184
  • Peer Reviewed
• [Journal Article] Clinical significance of CD24 as a predictor of bladder cancer recurrence (2013)
  • Author(s): Chunxiao Liu
  • Journal Title: Oncology Letters Volume: In press
  • Peer Reviewed
• [Journal Article] Cancer stem cell-like characteristics of a CD133+ subpopulation in the J82 human bladder cancer cell line (2013)
  • Author(s): Peng Huang
  • Journal Title: Molecular and Clinical Oncology Volume: 1 Pages: 180-184
  • Peer Reviewed
• [Journal Article] Preclinical biodistribution and safety study of REIC/Dkk-3 encoding adenovirus vector for prostate cancer gene therapy (2012)
  • Author(s): Sugimoto M Watanabe M, Kaku H, , , , , , , .Preclinical Li SA Noguchi H Ueki H Sakaguchi M Huh NH Nasu Y Kumon H
  • Journal Title: Oncol Rep Volume: 28 Pages: 1645-52
  • Peer Reviewed
• [Journal Article] Implications of transcriptional factor (2012)
  • Author(s): Huang P, Chen J, Wang L, Na Y,Kaku H, Ueki H, Sasaki K, Yamaguchi K, Zhang K, Saika T, Nasu Y, Watanabe M, Kumon H
  • Journal Title: OCT-4, in human bladder malignancy and tumor recurrence Volume: 29 Pages: 829-34
  • Peer Reviewed
• [Journal Article] REIC/Dkk-3-encoding adenoviral vector as a potentially effective therapeutic agent for bladder cancer (2012)
  • Author(s): Hirata T, Watanabe M, Kaku H, Kobayashi Y, Yamada H, Sakaguchi M, Takei K, Huh NH, Nasu Y, Kumon H
  • Journal Title: International Journal of Oncology Volume: 41 Pages: 559-64
  • Peer Reviewed
• [Journal Article] The hTERT promoter enhances the antitumor activity of an oncolytic adenovirus under a hypoxic microenvironment (2012)
  • Author(s): Hashimoto Y, Tazawa H, Teraishi F, Kojima T, Watanabe Y, Uno F, Yano S, Urata Y, Kagawa S, Fujiwara T
  • Journal Title: PLoS One Volume: 7
  • NAID: 120005232484
  • Peer Reviewed
• [Journal Article] In vivo imaging of human cancer with telomerase-specific replication-selective adenovirus (2012)
  • Author(s): Fujiwara T
  • Journal Title: Methods Mol Biol Volume: 872 Pages: 129-39
  • Peer Reviewed
• [Journal Article] A novel gene expression system for detecting viable bladder cancer cells (2012)
  • Author(s): Ueki H Watanabe M, Kaku H, , , , , , , , , , . Huang P Li SA Ochiai K Hirata T Noguchi H Yamada H Takei K Nasu Y Kashiwakura Y Kumon H
  • Journal Title: International Journal of Oncology Volume: 41 Pages: 135-40
  • Peer Reviewed
• [Journal Article] Implications of transcriptional factor, OCT-4, in human bladder malignancy and tumor recurrence (2012)
  • Author(s): Huang P Chen J Wang L Na Y Kaku H.Ueki H Sasaki K Yamaguchi K Zhang K Saika T Nasu Y Watanabe M, . Kumon H
  • Journal Title: Med Oncol Volume: 29 Pages: 829-34
  • Peer Reviewed
• [Journal Article] Implications of transcriptional factor, OCT-4, in human bladder malignancy and tumor recurrence (2012)
  • Author(s): Huang P
  • Journal Title: Med Oncol Volume: 29 Issue: 2 Pages: 828-34
  • DOI: 10.1007/s12032-011-9962-4
  • Peer Reviewed
• [Journal Article] adenoviral-mediated GFP genetic labeling of human cancer in the live mouse reports future recurrence after resection. (2011)
  • Author(s): Kishimoto H, Aki R, Urata Y, Bouvet M, Momiyama M, Tanaka N, Fujiwara T, Hoffman RM.
  • Journal Title: Cell Cycle Volume: 16 Pages: 2737-41
  • Peer Reviewed
• [Journal Article] Advanced two-step transcriptional amplification as a novel method for cancer-specific gene expression and imaging (2011)
  • Author(s): Watanabe M, Ueki H, Ochiai K, Huang P, Kobayashi Y, Nasu Y, Sasaki K,Kaku H, Kashiwakura Y, Kumon H.
  • Journal Title: Oncol Rep Volume: 26 Pages: 769-75
  • Peer Reviewed
• [Journal Article] REIC/Dkk-3遺伝子治療 (2011)
  • Author(s): 渡部昌実, 賀来春紀, 他
  • Journal Title: 前立腺癌(第2版)-基礎・臨床研究のアップデート- Pages: 559-563
• [Journal Article] In vivo biological purging for lymph node metastasis of human colorectal cancer by telomerase-specific oncolytic virotherapy. (2010)
  • Author(s): Kojima T, Watanabe Y, Hashimoto Y, Kuroda S, Yamasaki Y, Yano S, Ouchi M, Tazawa H, Uno F, Kagawa S, Kyo S, Mizuguchi H, Urata Y, Tanaka N, Fujiwara T.
  • Journal Title: Ann Surg Volume: 251 Pages: 1079-86
  • Peer Reviewed
• [Journal Article] Potent antitumor effects of combined therapy with a telomerase-specific, replication-competent adenovirus(OBP-301)and IL-2 in a mouse model of renal cell carcinoma (2010)
  • Author(s): Huang P Kaku H, , , , , , Chen J Kashiwakura Y Saika T Nasu Y Urata Y Fujiwara T, Watanabe M, . Potent antitumor Kumon H
  • Journal Title: Cancer Gene Ther Volume: 17 Pages: 484-91
  • Peer Reviewed
• [Journal Article] Potent antitumor effects of combined therapy with a telomerese-specific, replication-competent adenovirus(OBP-301) and IL-2 in a mouse model of renal cell carcinoma. (2010)
  • Author(s): Huang Peng
  • Journal Title: Cancer Gene Ther Volume: 17 Pages: 484-491
  • Peer Reviewed
• [Journal Article] Expression pattern of REIC/Dkk-3 in various cell types and the implications of the soluble form in prostatic acinar development. (2010)
  • Author(s): Zhang Kai
  • Journal Title: Int J Oncol Volume: 37 Pages: 1495-1501
  • Peer Reviewed
• [Presentation] 前立腺癌に対するREIC遺伝子治療-自己癌ワクチン化療法の確立を目指して (2013)
  • Author(s): 有吉勇一,平田武志,谷本竜太,佐々木克己,渡部昌実,江原 伸,那須保友,公文裕巳
  • Organizer: 泌尿器科分子・細胞研究会
  • Place of Presentation: 高知市
  • Year and Date: 2013-03-08
• [Presentation] REIC/Dkk-3 encoding adenoviral vector as a potentially effective therapeutic agent for bladder cancer (2012)
  • Author(s): Hirata T, Watanabe M, Kaku H, Ebara S, Watanabe T, Nasu Y, Kumon H
  • Organizer: 日本泌尿器科学会西日本総会
  • Place of Presentation: 徳島
  • Year and Date: 2012-11-09
• [Presentation] 新規がん治療遺伝子REIC/Ckk-3 -がんワクチン機能を有する遺伝子医薬 (2012)
  • Author(s): 渡部昌実 那須保友 公文裕巳
  • Organizer: BioJapan 2012 World Business Forum
  • Place of Presentation: 横浜
  • Year and Date: 2012-10-12
• [Presentation] 前立腺癌に対する遺伝子治療 -新たな免疫療法の開発をめざした取り組み (2011)
  • Author(s): 佐々木克己、那須保友、渡部昌実、賀来春紀、公文裕巳
  • Organizer: 第63回日本泌尿器科学会 西日本総会
  • Place of Presentation: 福岡
  • Year and Date: 2011-11-11
• [Presentation] 前立腺癌の治療(総論): 「前立腺癌早期発見早期診断プロジェクト」 (2011)
  • Author(s): 賀来 春紀
  • Organizer: フォローアップ協力セミナー
  • Place of Presentation: 中国・長春
  • Year and Date: 2011-10-15
• [Presentation] 前立腺癌の治療(各論): 「前立腺癌早期発見早期診断プロジェクト」 (2011)
  • Author(s): 賀来 春紀
  • Organizer: フォローアップ協力セミナー
  • Place of Presentation: 中国・長春
  • Year and Date: 2011-10-15
• [Presentation] 前立腺癌に対するREIC 遺伝子治療 (2011)
  • Author(s): 黄 鵬,渡部昌実,賀来春紀,陳 潔,那須保友,公文裕巳
  • Organizer: 癌免疫外科研究会
  • Place of Presentation: 和歌山市
  • Year and Date: 2011-05-19
• [Presentation] A phase I/IIstudy of adenovirus mediated interleukin-12 gene therapy for hormone refractory prostate cancer : (2011)
  • Author(s): Sasaki K, Nasu Y, Kaku H, Watanabe M, Nose H, Kanbara D, Saika T, Kumon H
  • Organizer: An interim report American Society of Clinical Oncology 2010 Genitourinary Cancers Symposium
  • Place of Presentation: Orlando, Florida, U.S.A.
  • Year and Date: 2011-02-03
• [Presentation] Cooperative Research on Genetic Predisposition for prostate Cancer in Asia by Analyzing Cumulative Association of SNPs The analyses data in Japan (2010)
  • Author(s): Huang P.
  • Organizer: 第4回 日中泌尿器科会議
  • Place of Presentation: 中国 西安
  • Year and Date: 2010-10-16
• [Presentation] Cooperative Research on Genetic Predisposition for prostate Cancer in Asia by Analyzing Cumulative Association of SNPs The analyses data in Chinese (2010)
  • Author(s): Wang L.
  • Organizer: 第4回 日中泌尿器科会議
  • Place of Presentation: 中国 西安
  • Year and Date: 2010-10-16
• [Presentation] Systemic Tcell activation following in situ gene therapy in prostate cancer patients (2010)
  • Author(s): Kurosaka S.
  • Organizer: 第4回 日中泌尿器科会議
  • Place of Presentation: 中国 西安
  • Year and Date: 2010-10-16
• [Presentation] Cooperative research on genetic predisposition for prostate cancer in Asia by analyzing cumulative association of SNPs-the analyses data in Japanese. (2010)
  • Author(s): Kaku H.
  • Organizer: 27th日韓泌尿器科会議
  • Place of Presentation: 京都
• [Presentation] A phase I/II study of adenovirus-mediated interleukin 12 gene therapy for hormone refractory prostate cancer : An interim report (2010)
  • Author(s): Sasaki K.
  • Organizer: 日本遺伝子治療学会学術集会
  • Place of Presentation: 宇都宮市,栃木県総合文化センター
• [Book] 前立腺癌(第2 版)-基礎・臨床研究のアップデート (2011)
  • Author(s): 渡部昌実、賀来春紀、黄 鵬 、那 須 保 友 、公文裕巳
  • Publisher: 日本臨牀社
• [Book] 前立腺癌の治療 遺伝子治療 自殺遺伝子・免疫遺伝子を用いた遺伝子治療 (2011)
  • Author(s): 那須保友、佐々木克己、賀来春紀、渡部昌実、公文裕巳
  • Publisher: 日本臨牀社