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Proteomic analysis of cyst fluid associated with renal cell carcinoma

Research Project

Project/Area Number 22591776
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Urology
Research InstitutionKitasato University

Principal Investigator

IWAMURA Masatsugu  北里大学, 医学部, 教授 (20176564)

Co-Investigator(Kenkyū-buntansha) FUJITA Tetsuo  北里大学, 医学部, 講師 (00306599)
TABATA Kenichi  北里大学, 医学部, 講師 (20327414)
NISHI Morihiro  北里大学, 医学部, 助教 (40383624)
WAKATABE Yoji  北里大学, 医学部, 助教 (10525710)
OKUSA Hiroshi  北里大学, 医学部, 助教 (70337963)
SASAMOTO Haruko  北里大学, 医学部, 助教 (70439115)
MATSUDA Daisuke  北里大学, 医学部, 助教 (50348570)
KODERA Yoshio  北里大学, 理学部, 准教授 (60265733)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords腎細胞癌 / 腫瘍マーカー / ペプチド解析 / プロテオーム解析 / 嚢胞液 / アガロース2次元電気泳動 / HPLC / 14-3-3 protein / ペプチド / プロテオーム
Research Abstract

Proteins in the cyst fluid were separated by reverse-phase high- performance liquid chromatography and agarose two- dimensional gel electrophoresis and were identified by tandem mass spectrometry. Our search resulted in the identification and selection of 14-3-3 protein beta/alpha, which was overexpressed in cyst fluid from cyst-associated RCC but has not been previously associated with RCC. The expression levels of 14 -3-3 protein beta/ alpha were higher in urine samples from patients with RCC than in samples from healthy volunteers, suggesting that 14-3-3 Protein beta/ alpha may be a diagnostically useful biomarker for early diagnosis of RCC.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (32 results)

All 2012 2011 2010 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (17 results) (of which Invited: 5 results) Book (1 results)

  • [Journal Article] Clayman, Positive Correlation Between Motion Analysis Data on the LapMentor Virtual Reality Laparoscopic Surgical Simulator and the Results from Videotape Assessment of Real Laparoscopic Surgeries2012

    • Author(s)
      Tadashi Matsuda, Elspeth M. McDougall,Yoshinari Ono, Ryohei Hattori, Shiro Baba, Masatsugu Iwamura, Toshiro Terachi, Seiji Naito, Ralph V
    • Journal Title

      JOURNAL OF ENDOUROLOGY

      Volume: 26(11) Pages: 1506-1511

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] C-reactive protein as a prognostic marker for advanced renal cell carcinoma treated with sunitinib2012

    • Author(s)
      Tetsuo Fujita, Masatsugu Iwamura, Daisuke Ishii, Kenichi Tabata, Kazumasa Matsumoto, Kazunari Yoshida, Shiro Baba
    • Journal Title

      INTERNATIONAL JOURNAL OF UROLOGY

      Volume: 19(10) Pages: 908-913

    • NAID

      10031123326

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Surgical and Oncological Outcomes of Laparoscopic Partial Nephrectomy:A Japanese Multi-Institutional Study of 1375 Patients2012

    • Author(s)
      Hideo Saito, Tadashi Matsuda, Kazunari Tanabe, Akihiro Kawauchi, Toshiro Terachi, Ken Nakagawa, Masatsugu Iwamura, Masanobu Shigeta, Katsunori Tatsugami, Akihiro Ito, Jiro Machida,Mutsushi Kawakita, Hidefumi Kinoshita,Nobuo Shinohara, Naomasa Ioritani,Toshimori Seki, Yoichi Arai
    • Journal Title

      JOURNAL OF ENDOUROLOGY

      Volume: 26(6) Pages: 652-659

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 小径腎腫瘍に対する腹腔鏡下腎部分切除術後腎機能に関する臨床的検討-無阻血・阻血手術の比較-2012

    • Author(s)
      西 盛宏、 岩村正嗣、 藤田哲夫、笹本 治子、山田泰史、馬場志郎
    • Journal Title

      Japanese Journal of Endourology and ESWL

      Volume: 25(2) Pages: 299-304

    • NAID

      130005003642

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cytocidal effects of acridine orange evoked by blue light on human bladder cancer cells2012

    • Author(s)
      Morihiro Nishi, HIromi Hiruma, Haruko Sasamoto, Masatsugu Iwamura, Risa Isonaka, Shiro Baba
    • Journal Title

      Kitasato Med J

      Volume: 42(2) Pages: 128-137

    • NAID

      40019429455

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 小径腎腫瘍に対する腹腔鏡下腎部分切除術後腎機能に関する臨床的検討―無阻血・阻血手術の比較―2012

    • Author(s)
      西盛宏、岩村正嗣、藤田哲夫、笹本治子、山田泰史、馬場志郎
    • Journal Title

      Japanese Journal of Endourology and ESWL

      Volume: 25(2) Pages: 299-304

    • NAID

      130005003642

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Erythema Multiforme Induced by Sorafenib for Metastatic Renal Cell Carcinoma2012

    • Author(s)
      Masaomi Ikeda,Tetsuo Fujita,Sumiyuki Mii,Ken-ichi Tanabe,Ken-ichi Tabata,Kazumasa Matsumoto,Takefumi Satoh,Masatsugu Iwamura
    • Journal Title

      JAPANESE JOURNAL OF CLINICAL ONCOLOGY

      Volume: 42(9) Pages: 820-824

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 14-3-3 Protein beta/alpha as a urinary biomarker for renal cell carcinoma : proteomic analysis of cyst fluid2011

    • Author(s)
      Satoru Minamida
    • Journal Title

      Analytical and Bioanalytical Chemistry

      Volume: 401 Pages: 245-252

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profilin 1 overexpression in renal cell carcinoma2011

    • Author(s)
      Satoru Minamida
    • Journal Title

      INTERNATIONAL JOURNAL OF UROLOGY

      Volume: 18 Pages: 63-71

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 腹腔鏡下腎盂形成術:初期50尿管の長期成績2011

    • Author(s)
      岩村正嗣
    • Journal Title

      Japanese Journal of Endourology and ESWL

      Volume: 24 Pages: 282-288

    • NAID

      130005003576

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of Fluoroquinolone-resistant Escherichia coli Before and Incidence of Acute Bacterial Prostatitis After Prostate Biopsy2011

    • Author(s)
      Satoru Minamida
    • Journal Title

      Ambulatory and Office Urology

      Volume: 78 Pages: 1235-1239

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profilin 1 overexpression in renal cell carcinoma2011

    • Author(s)
      Satoru Minamida, Masatsugu Iwamura, ほか7名
    • Journal Title

      International Journal of Urology

      Volume: 18 Pages: 63-71

    • NAID

      10027902072

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] High-yield peptide-extraction method for the discovery of subnanomolar biomarkers from small serum samples.2010

    • Author(s)
      Kawashima Y, ほか6名, Kodera Y
    • Journal Title

      J.Proteome Research

      Volume: 9 Pages: 694-705

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Proteomic study of sera from patients with bladder cancer -Usefulness of S100A8 and S100A9 proteins2010

    • Author(s)
      Minami S, ほか4名, Kodera Y
    • Journal Title

      Cancer Genomics Proteomics

      Volume: 7 Pages: 181-189

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 腹腔鏡下根治的左腎摘除術(経腹膜到達法)2012

    • Author(s)
      岩村正嗣
    • Organizer
      第19回泌尿器腹腔鏡ビデオ講習会
    • Place of Presentation
      東京
    • Year and Date
      2012-09-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Approach of the lower urinary system2012

    • Author(s)
      T.Piechaud, CC.Abbou, R.Van Velthoven, S.Egawa, M.Iwamura,SH.Lu, YC.Tsai, SM.Wang
    • Organizer
      ADVANCED COURSE IN LAPAROSCOPIC UROLOGICAL SURGERY
    • Place of Presentation
      台湾
    • Year and Date
      2012-08-31
    • Related Report
      2012 Final Research Report
  • [Presentation] 腎細胞癌に対するリンパ節郭清の意義: To do or not to do?2012

    • Author(s)
      岩村正嗣、藤田哲夫、南田 諭、馬場志郎
    • Organizer
      第100回日本泌尿器科学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2012-04-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Partial nephrectomy, ADVANCED COURSE IN LAPAROSCOPIC UROLOGICAL2011

    • Author(s)
      Masatsugu Iwamura
    • Organizer
      SURGERY
    • Place of Presentation
      台湾
    • Year and Date
      2011-09-03
    • Related Report
      2012 Final Research Report
  • [Presentation] Partial nephrectomy2011

    • Author(s)
      Masatsugu Iwamura
    • Organizer
      ADVANCED COURSE IN LAPAROSCOPIC UROLOGICAL SURGERY
    • Place of Presentation
      台湾(招待講演)
    • Year and Date
      2011-09-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cystotomy2011

    • Author(s)
      Masatsugu Iwamura
    • Organizer
      ADVANCED COURSE IN LAPAROSCOPIC UROLOGICAL SURGERY
    • Place of Presentation
      台湾
    • Year and Date
      2011-09-02
    • Related Report
      2012 Final Research Report
  • [Presentation] プロテオミクスの手法を用いた腎癌新規腫瘍マーカーの探索と多検体比較試験2011

    • Author(s)
      南田諭
    • Organizer
      第99回日本泌尿器科学会総会
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Year and Date
      2011-04-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] 腹腔鏡下腎盂形成術:経腹膜的アプローチによる Anderson-Hynes 法の手術手技2010

    • Author(s)
      岩村正嗣
    • Organizer
      第24回日本 Endourology ・ ESWL学会総会
    • Place of Presentation
      京都
    • Year and Date
      2010-10-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Structual maintenance of Chromsomes 3の膀胱癌新規腫瘍マーカーとしての有用性の検討2010

    • Author(s)
      大草洋、松本和将、服部学、小寺義男, ほか5名
    • Organizer
      第75回日本泌尿器科学会東部総会
    • Place of Presentation
      栃木県総合文化センター(宇都宮市)
    • Year and Date
      2010-09-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] 腹腔鏡下腎部分切除術の技術的問題点と近未来像2010

    • Author(s)
      岩村正嗣、藤田哲夫, ほか4名
    • Organizer
      第98回日本泌尿器科学会総会
    • Place of Presentation
      盛岡市民文化ホール・いわて県民情報交流センター、ホテルメトロポリタン盛岡本館/NEW WING・盛岡市アイスアリーナ(盛岡市)
    • Year and Date
      2010-04-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 血中ペプチドを対象とした各種がんの診断法開発を目指して2010

    • Author(s)
      小寺義男
    • Organizer
      第98回日本泌尿器科学会総会
    • Place of Presentation
      盛岡市民文化ホール・いわて県民情報交流センター、ホテルメトロポリタン盛岡本館/NEW WING・盛岡市アイスアリーナ(盛岡市)
    • Year and Date
      2010-04-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structual maintenance of Chromsomes 3の膀胱癌腫瘍マーカーとしての有用性の検討2010

    • Author(s)
      大草洋、松本和将、服部学、小寺義男、岩村正嗣, ほか8名
    • Organizer
      第98回日本泌尿器科学会総会
    • Place of Presentation
      盛岡市民文化ホール・いわて県民情報交流センター、ホテルメトロポリタン盛岡本館/NEW WING・盛岡市アイスアリーナ(盛岡市)
    • Year and Date
      2010-04-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 腎細胞癌の外科的治療法:腹腔鏡下腎部分切除術の問題点と将来展望

    • Author(s)
      岩村正嗣、石井大輔、藤田哲夫、吉田一成
    • Organizer
      第28回腎移植・血管外科研究会
    • Place of Presentation
      箱根
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Laparoscopic radical nephrectomy:points of technique

    • Author(s)
      M.Iwamura
    • Organizer
      ADVANCED COURSE IN LAPAROSCOPIC UROLOGICAL SURGERY
    • Place of Presentation
      台湾
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Approach of the lower urinary system

    • Author(s)
      T.Piechaud,CC.Abbou,R.Van Velthoven, S.Egawa,M.Iwamura,SH.Lu,YC.Tsai,SM.Wang
    • Organizer
      ADVANCED COURSE IN LAPAROSCOPIC UROLOGICAL SURGERY
    • Place of Presentation
      台湾
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 腹腔鏡下根治的左腎摘除術(経腹膜到達法)

    • Author(s)
      岩村正嗣
    • Organizer
      第19回泌尿器腹腔鏡ビデオ講習会
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 腎盂尿管移行部通過障害に対する腹腔鏡下腎盂形成術:15年の経験から学んだコツと工夫

    • Author(s)
      岩村正嗣
    • Organizer
      第20回Clinical Urology研究会
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] エレクトロニクスシリーズ次世代光医療 -レーザー技術の臨床への橋渡し-2010

    • Author(s)
      岩村正嗣、馬場志郎
    • Publisher
      シーエムシー出版
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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