| Project/Area Number |
22591776
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Tetsuo 北里大学, 医学部, 講師 (00306599)
TABATA Kenichi 北里大学, 医学部, 講師 (20327414)
NISHI Morihiro 北里大学, 医学部, 助教 (40383624)
WAKATABE Yoji 北里大学, 医学部, 助教 (10525710)
OKUSA Hiroshi 北里大学, 医学部, 助教 (70337963)
SASAMOTO Haruko 北里大学, 医学部, 助教 (70439115)
MATSUDA Daisuke 北里大学, 医学部, 助教 (50348570)
KODERA Yoshio 北里大学, 理学部, 准教授 (60265733)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 腎細胞癌 / 腫瘍マーカー / ペプチド解析 / プロテオーム解析 / 嚢胞液 / アガロース2次元電気泳動 / HPLC / 14-3-3 protein / ペプチド / プロテオーム |
|---|
| Research Abstract |
Proteins in the cyst fluid were separated by reverse-phase high- performance liquid chromatography and agarose two- dimensional gel electrophoresis and were identified by tandem mass spectrometry. Our search resulted in the identification and selection of 14-3-3 protein beta/alpha, which was overexpressed in cyst fluid from cyst-associated RCC but has not been previously associated with RCC. The expression levels of 14 -3-3 protein beta/ alpha were higher in urine samples from patients with RCC than in samples from healthy volunteers, suggesting that 14-3-3 Protein beta/ alpha may be a diagnostically useful biomarker for early diagnosis of RCC.
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