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Development of free activin assay for the diagnosis and treatment of FIRS

Research Project

Project/Area Number 22591816
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionGunma University

Principal Investigator

ABE Yumiko  群馬大学, 保健学研究科, 准教授 (70261857)

Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsアクチビン / フォリスタチン / FIRS / 絨毛膜羊膜炎 / 羊膜細胞 / インヒビン
Research Abstract

Chorioamnionitis is the primary disease of FIRS. Because the amnion is the avascular tissue, activin A produced in the amniotic cells is directly secreted into amniotic fluid, and seems to affect the fetus. Using cultured human amniotic cells with LPS or TNF-alpha, as a model system of amnionitis, we clarified that these factors stimulate activin A secretion from amniotic cells. A biologically active form of activin A is free activin A, which is not bound by the binding proteins follistatin or FSTL3. Therefore, we synthesized a peptide which has an amino acid sequence of follistatin/FSTL3-binding site of activin A, used it in immunization and obtained antibodies. The antibodies were purified by immunochromatography. Using the antibodies, we are analyzing the activin A secreted from amniotic cells by the stimulation of TNF-alpha.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (42 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (13 results) (of which Peer Reviewed: 7 results) Presentation (24 results) Book (2 results) Remarks (3 results)

  • [Journal Article] Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering2014

    • Author(s)
      Horii T, Arai Y, Yamazaki M, Morita S, Kimura M, Itoh M, Abe Y, Hatada I
    • Journal Title

      Sci Rep

      Volume: 4 Issue: 1 Pages: 4513-4513

    • DOI

      10.1038/srep04513

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genome engineering of mammalian haploid embryonic stem cells using the cas9/RNA system2013

    • Author(s)
      Horii T, Morita S, Kimura M, Kobayashi R, Tamura D, Takahashi RU, Kimura H, Suetake I, Ohata H, Okamoto K, Tajima S, Ochiya T, Abe Y, Hatada I
    • Journal Title

      PeerJ

      Volume: 2013 Issue: 1 Pages: e230-e230

    • DOI

      10.7717/peerj.230

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gram-negative bacterial lipopolysaccharide stimulates activin a secretion from human amniotic epithelial cells2013

    • Author(s)
      Abe Y, Marukawa R, Tsuru N, Sato M, Matsuda H, Sadakata H, Kameda T, Minegishi T
    • Journal Title

      Int J Endocrinol

      Volume: 2013 Pages: 789012-789012

    • DOI

      10.1155/2013/789012

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Activin A is stimulated by tumor necrosis factor-alpha and modulates collagen gene expression in human amniotic cells2013

    • Author(s)
      Abe Y, Komatsubara M, Saito M, Toda M, Shinozaki H, Tamura T, Kasahara Y, Sedakata H, Minegishi T
    • Journal Title

      J Endocrinol Invest

      Volume: 36(7) Pages: 515-20

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Important initiative roles of CD44 and Tenascin in Sampson's theory of the pathogenesis and development of endometriosis2013

    • Author(s)
      Igarashi S, Igarashi T, Abe Y, Liang SG, Minegishi T, Igarashi M
    • Journal Title

      Journal of Endometriosis and Pelvic Pain Disorders

      Volume: 5(3) Pages: 100-104

    • URL

      http://www.j-endometriosis.com/article/important-initiative-roles-of-cd44-and-tenascin-in-sampson-s-theory-of-the-pathogenesis-and-development-of-endometriosis

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 子宮内膜症組織の間質細胞培養系への腹水添加の意義・効果について2013

    • Author(s)
      五十嵐敏雄,佐川義英,古村絢子,寺田光二郎,嘉本寛江,中村泰昭,落合尚美,中川圭介,中江華子,梁善光,五十嵐茂雄,安部由美子,五十嵐正雄
    • Journal Title

      日本エンドメトリオーシス学会会誌

      Volume: 34巻 Pages: 211-214

    • URL

      http://www.endometriosis.gr.jp/non-member/kaishi/kaishi34pdf/56-ippan-kiso5-2.pdf

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] LH, FSH.内分泌・糖尿病2013

    • Author(s)
      安部由美子、峯岸敬
    • Journal Title

      代謝内科, (科学評論社)

      Volume: 第36巻, (特別増刊号) Pages: 153-159

    • URL

      http://www.kahyo.com/brand/b-Bzo201304-36S4

    • Related Report
      2013 Final Research Report
  • [Journal Article] Important initiative roles of CD44 and Tenascin in Sampson’s theory of the pathogenesis and development of endometriosis2013

    • Author(s)
      Igarashi S, Igarashi T, Abe Y, Liang SG, Minegishi T, Igarashi M
    • Journal Title

      Journal of Endometriosis and Pelvic Pain Disorders

      Volume: 5 Pages: 100-104

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] LH, FSH2013

    • Author(s)
      安部由美子、峯岸敬
    • Journal Title

      内分泌・糖尿病・代謝内科

      Volume: 第36巻特別増刊号 Pages: 153-159

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
  • [Journal Article] LH, FSH2012

    • Author(s)
      安部由美子、峯岸敬
    • Journal Title

      内分泌・糖尿病・代謝内科

      Volume: 34巻特別増刊号(in press)

    • Related Report
      2011 Annual Research Report
  • [Journal Article] 研究室紹介2011

    • Author(s)
      安部由美子
    • Journal Title

      日本生殖内分泌学会雑誌

      Volume: Vol.16 Pages: 36-37

    • URL

      http://www.seishoku.org/11_16kan/12-shoukai1.pdf

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Journal Article] 下垂体糖蛋白質ホルモン−αサブユニット(α-PGH), 広範囲血液・尿化学検査免疫学的検査–その数値をどう読むか-2010

    • Author(s)
      平井俊朗、安部由美子、田中滋康
    • Journal Title

      日本臨床68巻増刊号7 (全868頁)(通巻第982号)

      Pages: 216-219

    • URL

      http://www.nippon-rinsho.co.jp/backnum/z_mokuji/ketueki7-4.html

    • Related Report
      2013 Final Research Report
  • [Journal Article] 下垂体糖蛋白質ホルモン-αサブユニット(α-PGH)2010

    • Author(s)
      平井俊朗、安部由美子、田中滋康
    • Journal Title

      日本臨床

      Volume: 68(増7) Pages: 216-219

    • Related Report
      2010 Annual Research Report
  • [Presentation] 深部内膜症に対する3-ethylpyridine含有腟リングの効果2014

    • Author(s)
      五十嵐正雄、横田佳昌、五十嵐茂雄、安部由美子、五十嵐敏雄、梁善光
    • Organizer
      第35回日本エンドメトリオーシス学会
    • Place of Presentation
      鹿児島市
    • Related Report
      2013 Final Research Report
  • [Presentation] 深部内膜症に対する3-ethylpyridine 含有腟リングの効果2014

    • Author(s)
      五十嵐正雄、横田 佳昌、五十嵐茂雄、安部由美子、五十嵐敏雄、梁善光
    • Organizer
      第35回日本エンドメトリオーシス学会
    • Place of Presentation
      鹿児島市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 経腟エコーとMRIで診断困難な深部内膜症の診断と新しい治療法2013

    • Author(s)
      五十嵐正雄、横田佳昌、五十嵐敏雄、五十嵐茂雄、安部由美子、梁善光、峯岸敬
    • Organizer
      第34回日本エンドメトリオーシス学会
    • Place of Presentation
      宇都宮市
    • Year and Date
      2013-01-19
    • Related Report
      2013 Final Research Report
  • [Presentation] CRISPR/Cas系を利用したノックアウトマウス直接作成法の検討2013

    • Author(s)
      山﨑美帆、堀居拓郎、荒井勇二、森田純代、木村美香、伊藤理廣、安部由美子、畑田出穂
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸市
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 半数体胚由来2倍体ES細胞(hddES)-再生医療における新たな多能性幹細胞の選択枝2013

    • Author(s)
      小林遼平、堀居拓郎、田村大樹、森田純代、木村美香、安部由美子、畑田出穂
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸市
    • Related Report
      2013 Final Research Report
  • [Presentation] CRISPR /Cas9システムによる哺乳類半数体ES細胞のゲノム操作2013

    • Author(s)
      堀居拓郎、森田純代、木村美香、小林遼平、田村大樹、高橋陵宇、木村博信、末武勲、大畑広和、岡本康司、田嶋正二、落谷孝広、安部由美子、畑田出穂
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸市
    • Related Report
      2013 Final Research Report
  • [Presentation] 半数体ES細胞とCRISPR/Casを用いた高効率ゲノム編集2013

    • Author(s)
      堀居拓郎、森田純代、木村美香、小林遼平、田村大樹、高橋陵宇、木村博信、末武勲、大畑広和、岡本康司、田嶋正二、落谷孝広、安部由美子、畑田出穂
    • Organizer
      第3回ゲノム編集研究会
    • Place of Presentation
      広島市
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト羊膜培養細胞におけるアクチビンAの作用2013

    • Author(s)
      荒井麻希、髙栁彰子、古川涼、大庭僚将、山中行義、小島望,小島萌生,嶋田佳奈,渡邉和子、安部由美子
    • Organizer
      第38回日本比較内分泌学会
    • Place of Presentation
      宮崎市
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 小分子RNA配列と相補的なDNAを切断するCRISPR/Cas9ヌクレアーゼを用いた高効率ゲノム編集2013

    • Author(s)
      堀居拓郎、森田純代、木村美香、田村大樹、小林遼平、山﨑美帆、家坂直子、高橋陵宇、木村博信、末武勲、大畑広和、岡本康司、田嶋正二、落谷孝広、峯岸敬、伊藤理廣、安部由美子、畑田出穂
    • Organizer
      第5回日本RNAi研究会
    • Place of Presentation
      広島市
    • Related Report
      2013 Final Research Report
  • [Presentation] 半数体胚由来2倍体ES細胞(hddES)-再生医療における新たな多能性幹細胞の選択枝2013

    • Author(s)
      小林遼平、堀居拓郎、田村大樹、森田純代、木村美香、安部由美子、畑田出穂
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 経腟エコーとMRIで診断困難な深部内膜症の診断と新しい治療法2013

    • Author(s)
      五十嵐正雄、横田佳昌、五十嵐敏雄、五十嵐茂雄、安部由美子、梁善光、峯岸敬
    • Organizer
      第34回日本エンドメトリオーシス学会
    • Place of Presentation
      宇都宮
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト羊膜培養細胞におけるinhibin, activin, follistatin2012

    • Author(s)
      髙栁彰子、荒井麻希、古川涼、大橋直人、鈴木宏和、山中行義、安部由美子
    • Organizer
      第37回日本比較内分泌学会
    • Place of Presentation
      福井市
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト羊膜細胞における2,3,7,8-tetrachlorodibenzo-p-dioxinの作用2012

    • Author(s)
      大橋直人、鈴木宏和、山中行義、荒井麻希、髙栁彰子、古川涼、安部由美子、水谷哲也、宮本薫
    • Organizer
      第37回日本比較内分泌学会
    • Place of Presentation
      福井市
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト羊膜培養細胞におけるinhibin, activin, follistatin2012

    • Author(s)
      髙栁彰子、荒井麻希、古川涼、大橋直人、鈴木宏和、山中行義、安部由美子
    • Organizer
      第37回日本比較内分泌学会
    • Place of Presentation
      福井
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト羊膜細胞における2,3,7,8-tetrachlorodibenzo-p-dioxinの作用2012

    • Author(s)
      大橋直人、鈴木宏和、山中行義、荒井麻希、髙栁彰子、古川涼、安部由美子、水谷哲也、宮本薫
    • Organizer
      第37回日本比較内分泌学会
    • Place of Presentation
      福井
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト羊膜間葉系細胞と上皮細胞におけるTNF-alphaのactivin A発現促進作用2011

    • Author(s)
      鈴木ちかる、荒井麻希、高柳彰子、古川涼、笠原慶充、田村友宏、定方久延、勝俣祐介、峯岸敬、安部由美子
    • Organizer
      第16回日本生殖内分泌学会
    • Place of Presentation
      東京
    • Year and Date
      2011-11-19
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] 絨毛膜羊膜炎モデルとしてのヒト羊膜細胞培養系2011

    • Author(s)
      鈴木ちかる、荒井麻希、高柳彰子、古川涼、水流奈己、丸川りさ、小松原麻紀、安部由美子
    • Organizer
      第48回関東甲信地区医学検査学会
    • Place of Presentation
      前橋
    • Year and Date
      2011-10-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] 絨毛膜羊膜炎モデルとしてのヒト羊膜細胞培養系2011

    • Author(s)
      鈴木ちかる、荒井麻希、高柳彰子、古川涼、水流奈己、丸川りさ、小松原麻紀、安部由美子
    • Organizer
      第48回関東甲信地区医学検査学会
    • Place of Presentation
      前橋市
    • Related Report
      2013 Final Research Report
  • [Presentation] 子宮腺筋症・深部内膜症・卵巣内膜症に対する新薬3剤単独経腟的局所直接注射療法2011

    • Author(s)
      五十嵐正雄、横田佳昌、五十嵐敏雄、安部由美子、五十嵐茂雄、梁善幸
    • Organizer
      第32回日本エンドメトリオーシス学会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Novel transvaginal echo-guided injection treatment for adenomyosis, vaginal and deep endometriosis, and ovarian endometriosis with danazol, 3-ethyl pyridine, or valproic acid.2010

    • Author(s)
      Igarashi M, Yokota Y, Igarashi T, Igarashi S, Abe Y, Liang SG.
    • Organizer
      The First Asian Conference on Endometriosis
    • Place of Presentation
      Shanghai,中国
    • Year and Date
      2010-10-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Endocrine disruptor dioxin affects gene expression related to collagen metabolism in human amniotic cells.2010

    • Author(s)
      Yumiko Abe, Nami Tsuru, Risa Marukawa, Chikaru Suzuki, Maki Komatsubara, Megumi Saito, Mayuho Toda, Tetsuya Mizutani
    • Organizer
      28^<th> International Congress of the Medical Women's International Association
    • Place of Presentation
      Munster ドイツ
    • Year and Date
      2010-07-29
    • Related Report
      2010 Annual Research Report
  • [Presentation] Novel transvaginal echo-guided injection treatment for adenomyosis, vaginal and deep endometriosis, and ovarian endometriosis with danazol, 3-ethyl pyridine, or valproic acid2010

    • Author(s)
      Igarashi M, Yokota Y, Igarashi T, Igarashi S, Abe Y, Liang SG
    • Organizer
      The First Asian Conference on Endometriosis
    • Place of Presentation
      Shanghai
    • Related Report
      2013 Final Research Report
  • [Presentation] 羊膜培養細胞のinhibin産生の検討2010

    • Author(s)
      水流奈己、丸川りさ、鈴木ちかる、小松原麻紀、齋藤恵美、戸田麻友穂、長谷川喜久、安部由美子
    • Organizer
      第57回日本臨床検査医学会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] Endocrine disruptor dioxin affects gene expression related to collagen metabolism in human amniotic cells2010

    • Author(s)
      Abe Y, Tsuru N, Marukawa R, Suzuki C, Komatsubara M, Saito M, Toda M, Mizutani T
    • Organizer
      28^<th> International Congress of the Medical Women's International Association
    • Place of Presentation
      Münster, Germany
    • Related Report
      2013 Final Research Report
  • [Book] 生物の事典, (生殖医療)2010

    • Author(s)
      安部由美子, 石原勝敏, 末光隆志, (総編集)
    • Publisher
      朝倉書店, (東京)
    • Related Report
      2013 Final Research Report
  • [Book] 生物の事典2010

    • Author(s)
      安部由美子
    • Total Pages
      542
    • Publisher
      朝倉書店(分担執筆)
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.health.gunma-u.ac.jp/

    • Related Report
      2013 Final Research Report
  • [Remarks] 群馬大学大学院保健学研究科/医学部保健学科

    • URL

      http://www.health.gunma-u.ac.jp/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.health.gunma-u.ac.jp/unit/#cB

    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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