Identification of the microRNA which is involved in theanti-proliferative action of estrogens

• Project/Area Number: 22591819
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: University of Yamanashi
• Principal Investigator: MITSUI Tetsuo 山梨大学, 大学院・医学工学総合研究部, 助教 (20402084)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: エストロジェン / 下垂体 / 細胞増殖 / microRNA / シグナル伝達

Research Abstract

Estrogens stimulate cell proliferation of estrogen-responsiveorgans including the uterus, mammary gland, and anterior pituitary gland. Recently, wehave found that 17-β estradiol (E2) had estrogen receptor-mediated anti-mitogenic actionin presence of growth factors such as insulin-like growth factor-1 (IGF-1) on ratlactotroph primary cell culture. However, at present, the cellular and molecularmechanisms underlying the IGF-1-dependent anti-mitogenic action of estrogen uponlactotroph remain unknown. In this study, we have found the microRNA which might beinvolved in the anti-proliferative action of estrogens.

Research Products

• [Journal Article] Mechanism forthe anti-proliferative action of estrogen onlactotroph proliferation (2012)
  • Author(s): Mitsui T, Ishida M, Arita J
  • Journal Title: J. Physiol. Sci. Volume: 62
• [Journal Article] Mechanism for the anti-proliferative action of estrogen on lactotroph proliferation (2012)
  • Author(s): Tetsuo Mitsui
  • Journal Title: Journal of Physiological Sciences Volume: 62
• [Journal Article] Inhibition of Bcl3 gene expressionmediates the anti-proliferative action ofestrogen in pituitary lactotrophs in primaryculture (2011)
  • Author(s): Mitsui T, Ishida M, Izawa M, Kagami Y,Arita J
  • Journal Title: Mol. Cell. Endocrinol Volume: 345 Pages: 68-78
  • Peer Reviewed
• [Journal Article] cAMP response element-bindingprotein (CREB) is required for epidermalgrowth factor (EGF)-induced cellproliferation and serum response elementactivation in neural stem cells isolated fromthe forebrain subventricular zone of adultmice. (2011)
  • Author(s): Iguchi H, Mitsui T, Ishida M, Kanba S,Arita J
  • Journal Title: Endocr. J. Volume: 58 Pages: 747-59
  • Peer Reviewed
• [Journal Article] Fetal exposure to2,3,7,8-tetrachlorodibenzo-p-dioxininduces expression of the chemokine genesCxcl4 and Cxcl7 in the perinatal mousebrain (2011)
  • Author(s): Mitsui T, Taniguchi N, Kawasaki N,Kagami Y, Arita J
  • Journal Title: J. Appl. Toxicol. Volume: 31 Pages: 279-284
  • Peer Reviewed
• [Journal Article] Fetal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin induces expression of the chemokine genes Cxcl4 and Cxcl7 in the perinatal mouse brain (2011)
  • Author(s): Mitsui T, Taniguchi N, Kawasaki N, Kagami Y, Arita J
  • Journal Title: Journal of applied toxicology Volume: 31 Pages: 279-284
  • Peer Reviewed
• [Journal Article] cAMP response element-binding protein (CREB) is required for epidermal growth factor (EGF)-induced cell proliferation and serum response element activation in neural stem cells isolated from the forebrain subventricular zone of adult mice (2011)
  • Author(s): Iguchi H, Mitsui T, Ishida M, Kanba S, Arita J
  • Journal Title: Endocrine Journal Volume: 58 Pages: 747-759
  • NAID: 10029862663
  • Peer Reviewed
• [Journal Article] Inhibition of Bcl3 gene expression mediates the anti-proliferative action of estrogen in pituitary lactotrophs in primary culture (2011)
  • Author(s): Mitsui T, Ishida M, Izawa M, Kagami Y, Arita J
  • Journal Title: Molecular and Cellular Endocrinology Volume: 345 Pages: 68-78
  • Peer Reviewed
• [Journal Article] Absence of ligand-independenttranscriptional activation of the estrogenreceptor via the estrogen response elementin pituitary lactotrophs in primary culture (2010)
  • Author(s): Ishida M, Mitsui T, Izawa M, Arita J
  • Journal Title: J.Steroid Biochem. Mol. Biol. Volume: 118 Pages: 93-101
  • Peer Reviewed
• [Presentation] プロラクチン産生細胞の細胞増殖に対するエストロジェンの抑制作用の発現機構 (2012)
  • Author(s): 三井哲雄、 石田真帆、 有田順
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本
  • Year and Date: 2012-03-30
• [Presentation] プロラクチン産生細胞の細胞増殖に対するエストロジェンの抑制作用の発現機構 (2012)
  • Author(s): 三井哲雄
  • Organizer: 第89回日本生理学会大会
  • Place of Presentation: 松本文化会館(長野県)
  • Year and Date: 2012-03-30
• [Presentation] IGF-1 依存性のエストロジェンによる増殖抑制作用には Bcl3 遺伝子発現の抑制が関与する (2011)
  • Author(s): 三井哲雄、石田真帆、有田順
  • Organizer: 第88回日本生理学会大会 第116回日本解剖学会総会・全国学術集会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-29
• [Presentation] Wistar系及び Wistar/Kyoto 系ラットにおけるプロラクチン産生細胞増殖及び遺伝子発現に対するエストラジオール及びIGF-1 の作用 (2011)
  • Author(s): 有田順、石田真帆、三井哲雄
  • Organizer: 第88回日本生理学会大会 第116回日本解剖学会総会・全国学術集会 合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-29
• [Presentation] IGF-1依存性のエストロゲンによる増殖抑制作用にはBcl3遺伝子発現の抑制が関与する (2011)
  • Author(s): 三井哲雄
  • Organizer: 第88回日本生理学会大会第116回日本解剖学会総会・全国学術集会合同大会
  • Place of Presentation: パシフィコ横浜(東日本大震災の為誌上開催)
  • Year and Date: 2011-03-29
• [Presentation] Absence of ligand-independenttranscriptional activation of the estrogenreceptor via the estrogen response elementin pituitary lactotrophs in primary culture (2010)
  • Author(s): Ishida M, Mitsui T, Izawa M, Arita J
  • Organizer: 14th International Congress ofEndocrinology
  • Place of Presentation: 京都
  • Year and Date: 2010-03-29
• [Remarks]
  • URL: http://www.med.yamanashi.ac.jp/basic/physio01/
• [Remarks] 山梨大学大学院医学工学総合研究部生理学講座第1教室ホームページ
  • URL: http://www.med.yamanashi.ac.jp/basic/physio01/
• [Remarks]
  • URL: http://www.med.yamanashi.ac.jp/basic/physio01/
• [Remarks]
  • URL: http://www.med.yamanashi.ac.jp/basic/physio01/