Identification of molecular marker for placenta accreta

• Project/Area Number: 22591827
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Nagasaki University
• Principal Investigator: MASUZAKI Hideaki 長崎大学, 大学院・医歯薬学総合研究科, 教授 (00173740)
• Co-Investigator(Kenkyū-buntansha): MIURA Kiyonori 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (00363490)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 癒着胎盤 / 胎盤 / mRNA / microRNA / 術中超音波検査 / 母体血漿 / 産婦人科 / 超音波診断 / 分子マーカー / RNA

Research Abstract

In this study, by high-throughput comparative analysis, placenta specific mRNAs/microRNAs (miRNAs) were identified. Regarding the placenta specific mRNAs, human placental lactogen (hPL) levels in maternal plasma may be a predictive marker for placenta accrete with total hysterectomy. Regarding the placenta specific microRNAs (miRNAs), most of them located on chromosome 19 microRNAs cluster region (C19MC). The expression levels of miRNAs on C19MC were increasing as the progress of pregnancy, while the expression profiling of them were not changed. The circulating levels of placenta specific miRNAs on C19MC in maternal plasma may be predictive markers for the risk of placenta accreta in cases of placenta previa. Furthermore, the intraoperative ultrasound examination is expected to be useful for evaluating the degree of placenta accrete in cases of placenta previa.

Research Products

• [Journal Article] Characterization ofplacenta-specific microRNAs in fetal growth restriction pregnancy (2013)
  • Author(s): Higashijima A, Miura K, Mishima H, Kinoshita A, Jo O, Abe S, Hasegawa Y, MiuraS, Yamasaki K, Yoshida A, Yoshiura K, Masuzaki H
  • Journal Title: Prenat Diagn Volume: 33 Pages: 214-222
• [Journal Article] Mother-to-child transmission of human T-cell lymphotropic virus type 1 (2013)
  • Author(s): Moriuchi H, Masuzaki H, Doi H, Katamine S
  • Journal Title: Pediatr Infect Dis J Volume: 32 Pages: 175-177
  • NAID: 120006985755
• [Journal Article] Pelvic pain in women with ovarian endometrioma is mostly associated withcoexisting peritoneal lesions (2013)
  • Author(s): Khan KN, Kitajima M, Fujishita A, Hiraki K, Matsumoto A, Nakashima M, Masuzaki H
  • Journal Title: Hum Reprod Volume: 28 Pages: 109-118
  • NAID: 120006985796
• [Journal Article] Copy number variation of the antimicrobial-gene, defensin beta 4, is associated with susceptibility to cervical cancer. (2013)
  • Author(s): Abe, S, Miura, K, Kinoshita, A, Mishima, H, Miura, S, Yamasaki, K, Hasegawa, Y, Higashijima, A, Jo, O, Sasaki, K, Yoshida, A, Yoshiura, KI, Masuzaki.
  • Journal Title: Journal of Human Genetics Volume: 58 Issue: 5 Pages: 250-253
  • DOI: 10.1038/jhg.2013.7
  • Peer Reviewed
• [Journal Article] Characterization of placenta-specific microRNAs in fetal growth restriction pregnancy (2013)
  • Author(s): Higashijima A
  • Journal Title: Prenat Diagn Volume: 33 Issue: 3 Pages: 214-222
  • DOI: 10.1002/pd.4045
  • Peer Reviewed
• [Journal Article] Pelvic pain in women with ovarian endometrioma is mostly associated with coexisting peritoneal lesions (2013)
  • Author(s): Khan KN
  • Journal Title: Hum Reprod Volume: 28 Pages: 109-118
  • DOI: 10.2147/ijwh.s35573
  • Peer Reviewed
• [Journal Article] Role of prostaglandin E2 in bacterial growth in women with endometriosis (2012)
  • Author(s): Khan KN, Kitajima M, Yamaguchi N, Fujishita A, Nakashima M, Ishimaru T,Masuzaki H
  • Journal Title: Hum Reprod Volume: 27 Pages: 3417-3424
  • NAID: 120006985795
• [Journal Article] Significance of p53-binding protein 1 nuclear foci inuterine cervical lesions: endogenous DNA double strand breaks and genomicinstability during carcinogenesis (2011)
  • Author(s): Matsuda K, Miura S, Kurashige T, Suzuki K, Kondo H, Ihara M, Nakajima H,Masuzaki H, Nakashima M
  • Journal Title: Histopathology Volume: 59 Pages: 441-451
• [Journal Article] Pre-vaccinationepidemiology of human papillomavirus infections in Japanese women with abnormal cytology (2011)
  • Author(s): Yamasaki K, Miura K, Shimada T, Ikemoto R, Miura S, Murakami M, Sameshima T,Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H
  • Journal Title: J Obstet Gynaecol Res Volume: 37 Pages: 1666-1670
• [Journal Article] Changes inserum anti-Mullerian hormone levels may predict damage to residual normal ovarian tissue after laparoscopic surgery for women with ovarian endometrioma (2011)
  • Author(s): Kitajima M, Khan KN, Hiraki K, Inoue T, Fujishita A, Masuzaki H
  • Journal Title: Fertil Steril Volume: 95 Pages: 2589-2591
• [Journal Article] Clinicalapplication of fetal sex determination using cell-free fetal DNA in pregnant carriers of X-linked genetic disorders (2011)
  • Author(s): Miura K, Higashijima A, Shimada T, Miura S, Yamasaki K, Abe S, Jo O, KinoshitaA, Yoshida A, Yoshimura S, Niikawa N, Yoshiura K, Masuzaki H
  • Journal Title: J Hum Genet Volume: 56 Pages: 296-299
• [Journal Article] Epidemiology of human papillomavirus genotypes in pregnant Japanese women (2011)
  • Author(s): Yamasaki K, Miura K, Shimada T, Miura S, Abe S, Murakami M, Sameshima T,Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H
  • Journal Title: J Hum Genet Volume: 56 Pages: 313-315
  • NAID: 10030658951
• [Journal Article] Clinical application of fetal sex determination using cell-free fetal DNA in pregnant carriers of X-linked genetic disorders (2011)
  • Author(s): Miura K, Higashijima A, Shimada T, Miura S, Yamasaki K, Abe S, Jo O, Kinoshita A, Yoshida A, Yoshimura S, Niikawa N, Yoshiura K, Masuzaki H
  • Journal Title: Journal of Human Genetics Volume: 56 Pages: 296-299
  • NAID: 40018765629
  • Peer Reviewed
• [Journal Article] Epidemiology of human papillomavirus genotypes in pregnant Japanese women (2011)
  • Author(s): Yamasaki K, Miura K, Shimada T, Miura S, Abe S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H
  • Journal Title: Journal of Human Genetics Volume: 56 Pages: 313-315
  • NAID: 10030658951
  • Peer Reviewed
• [Journal Article] Pre-vaccination epidemiology of human papillomavirus infections in Japanese women with a bnormal cytology (2011)
  • Author(s): Yamasaki K, Miura K, Shimada T, Ikemoto R, Miura S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H
  • Journal Title: Journal of Obstetrics and Gynaecology Research Volume: 37 Pages: 1666-1670
  • Peer Reviewed
• [Journal Article] Clinical application of fetal sex determination using cell-free fetal DNA in pregnant carriers of X-linked genetic disorders. (2011)
  • Author(s): Miura K, Higashijima A, Shimada T, Miura S, Yamasaki K, Abe S, Jo O, Kinoshita A, Yoshida A, Yoshimura S, Niikawa N, Yoshiura K, Masuzaki H.
  • Journal Title: Journal of Human Genetics Volume: (in press 掲載確定)
  • NAID: 40018765629
  • Peer Reviewed
• [Journal Article] Epidemiology of human papillomavirus genotypes in pregnant Japanese women. (2011)
  • Author(s): Yamasaki K, Miura K, Shimada T, Miura S, Abe S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H.
  • Journal Title: Journal of Human Genetics Volume: (in press 掲載確定)
  • NAID: 10030658951
  • Peer Reviewed
• [Journal Article] Pre-vaccination epidemiology of human papillomavirus infections in Japanese women with abnormal cytology. (2011)
  • Author(s): Yamasaki K, Miura K, Shimada T, Ikemoto R, Miura S, Murakami M, Sameshima T, Fujishita A, Kotera K, Kinoshita A, Yoshiura K, Masuzaki H.
  • Journal Title: The Journal of Obstetrics and Gynaecology Research Volume: (in press 掲載確定)
  • NAID: 120006985461
  • Peer Reviewed
• [Journal Article] Cell proliferation effect of GnRH agonist on pathological lesions of women with endometriosis, adenomyosis and uterine myoma (2010)
  • Author(s): Khan KN, Kitajima M, Hiraki K, Fujishita A, Nakashima M, Ishimaru T, Masuzaki H
  • Journal Title: Hum Reprod Volume: 25 Pages: 2878-2890
  • NAID: 120006984904
• [Journal Article] Identification of pregnancy-associated microRNAs in maternal plasma (2010)
  • Author(s): Miura K, Miura S, Yamasaki K, Higashijima A, Kinoshita A, Yoshiura K,Masuzaki H
  • Journal Title: Clin Chem Volume: 56 Pages: 1767-1771
• [Journal Article] The possibility of microarray-based analysis using cell-freeplacental mRNA in maternal plasma (2010)
  • Author(s): Miura K, Miura S, Yamasaki K, Shimada T, Kinoshita A, Niikawa N, Yoshiura K, Masuzaki H
  • Journal Title: Prenat Diagn Volume: 30 Pages: 849-861
• [Journal Article] Escherichia coli contamination of menstrual blood and effect of bacterial endotoxin on endometriosis (2010)
  • Author(s): Khan KN, Kitajima M, Hiraki K, Yamaguchi N, Katamine S, Matsuyama T,Nakashima M, Fujishita A, Ishimaru T, Masuzaki H
  • Journal Title: FertilSteril Volume: 94 Pages: 2860-2863
  • NAID: 120006984493
• [Journal Article] Fetalresponse to induced maternal emotions (2010)
  • Author(s): Araki M, Nishitani S, Ushimaru K, Masuzaki H, Oishi K, Shinohara K
  • Journal Title: J Physiol Sci Volume: 60 Pages: 213-220
  • NAID: 10027711852
• [Journal Article] Human papillomavirus DNA in plasma of patients with HPV16 DNA-positive uterinecervical cancer (2010)
  • Author(s): Shimada T, Yamaguchi N, Nishida N, Yamasaki K, Miura K, Katamine S, Masuzaki H
  • Journal Title: Jpn J Clin Oncol Volume: 40 Pages: 420-424
• [Journal Article] A case of Kallmann syndrome carrying a missense mutation in alternatively splicedexon 8A encoding the immunoglobulin-like domain IIIb of fibroblast growth factor receptor 1 (2010)
  • Author(s): Miura K, Miura S, Yoshiura K, Seminara S, Hamaguchi D, Niikawa N, Masuzaki H
  • Journal Title: Hum Reprod Volume: 25 Pages: 1076-1080
• [Journal Article] A case of Kallmann syndrome carrying a missense mutation in alternatively spliced exon 8A encoding the immunoglobulin-like domain IIIb of FGFR1. (2010)
  • Author(s): Miura K, Miura S, Yoshiura K, Seminara S, Hamaguchi D, Niikawa N, Masuzaki H.
  • Journal Title: Human Reproduction Volume: 25 Pages: 1076-1080
  • Peer Reviewed
• [Journal Article] The possibility of microarray-based analysis using cell-free placental mRNA in maternal plasma. (2010)
  • Author(s): Miura K, Miura S, Yamasaki K, Shimada T, Kinoshita A, Niikawa N, Yoshiura K, Masuzaki H.
  • Journal Title: Prenatal Diagnosis Volume: 30 Pages: 849-861
  • Peer Reviewed
• [Journal Article] Identification of pregnancy-associated microRNAs in maternal plasma. (2010)
  • Author(s): Miura K, Miura S, Yamasaki K, Higashijima A, Kinoshita A, Yoshiura K, Masuzaki H.
  • Journal Title: Clinical Chemistry Volume: 56 Pages: 1767-1771
  • Peer Reviewed
• [Journal Article] Human Papillomavirus DNA in Plasma of Patients with HPV16 DNA-positive Uterine Cervical Cancer. (2010)
  • Author(s): Shimada T, Yamaguchi N, Nishida N, Yamasaki K, Miura K, Katamine S, Masuzaki H.
  • Journal Title: Jpn J Clin Oncol Volume: 40 Pages: 420-424
  • NAID: 120006984483
  • Peer Reviewed
• [Journal Article] Placental multiple chorionic cysts in maternal scleroderma. (2010)
  • Author(s): Shimada T, Inoue T, Abe S, Hiraki K, Miura K, Hayashi T, Masuzaki H.
  • Journal Title: J Med Ultrasonics Volume: 37 Pages: 209-212
  • NAID: 10027722091
  • Peer Reviewed
• [Journal Article] 出生前診断の諸問題 (2010)
  • Author(s): 増崎英明
  • Journal Title: 産婦人科治療 Volume: 100 Pages: 33-36
• [Journal Article] Nuchal Translucencyの計測と意義 (2010)
  • Author(s): 増崎英明
  • Journal Title: 臨床婦人科産科 Volume: 64 Pages: 537-543
• [Journal Article] 外来診療マニュアル 周産期 5.出生前胎児スクリーニング b)染色体異常の超音波診断 (2010)
  • Author(s): 増崎英明
  • Journal Title: 産婦人科の実際 Volume: 59 Pages: 1635-1641
• [Journal Article] Cell-free placental mRNA/microRNAの臨床的意義とその応用 (2010)
  • Author(s): 三浦清徳、増崎英明
  • Journal Title: 産婦人科の実際 Volume: 59 Pages: 2043-2049
• [Journal Article] -妊産婦救急疾患- アナフィラキシーショックへの対応 (2010)
  • Author(s): 三浦清徳、増崎英明
  • Journal Title: 周産期医学 Volume: 40 Pages: 727-731
• [Journal Article] Copy numbervariation of the antimicrobial-gene, defensin beta 4, is associated withsusceptibility to cervical cancer
  • Author(s): Abe S, Miura K, Kinoshita A, Mishima H, Miura S, Yamasaki K, Hasegawa Y,Higashijima A, Jo O, Sasaki K, Yoshida A, Yoshiura KI, Masuzaki H
  • Journal Title: J Hum Genet Volume: (in press)
• [Presentation] 正常妊娠と胞状奇胎の双胎妊娠後に認められた胎盤部トロホブラスト腫瘍の一例 (2011)
  • Author(s): 東島愛、吉田敦、城大空、阿部修平、谷川輝美、三浦生子、山崎健太郎、吉村秀一郎、増崎英明
  • Organizer: 第29回日本絨毛性疾患研究会
  • Place of Presentation: 東京ステーションコンファレンス(東京)
  • Year and Date: 2011-09-30
• [Presentation] 大動脈クランプが著効した前置癒着胎盤の一例 (2011)
  • Author(s): 吉田 敦、山崎健太郎、阿部修平、三浦清徳、吉村秀一郎、増崎英明
  • Organizer: 第47回日本周産期・新生児医学会
  • Place of Presentation: 札幌
• [Presentation] 帝王切開術における術中超音波検査 (2011)
  • Author(s): 吉田 敦、谷川輝美、三浦清徳、吉村秀一郎、増崎英明
  • Organizer: 第63回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪
• [Presentation] 大動脈クランプが著効した前置癒着胎盤の一例 (2011)
  • Author(s): 吉田敦、山崎健太郎、阿部修平、三浦清徳、吉村秀一郎、増崎英明
  • Organizer: 第47回日本周産期・新生児医学会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
• [Presentation] 帝王切開術における術中超音波検査 (2011)
  • Author(s): 吉田敦、谷川輝美、三浦清徳、吉村秀一郎、増崎英明
  • Organizer: 第63回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪国際会議場(大阪)
• [Presentation] 全胞状奇胎特異的microRNAの網羅的解析 (2011)
  • Author(s): 三浦清徳、長谷川ゆり、東島愛、城大空、阿部修平、三浦生子、山崎健太郎、吉田敦、吉村秀一郎、吉浦孝一郎、増崎英明
  • Organizer: 第63回日本産科婦人科学会学術講演会
  • Place of Presentation: 大阪国際会議場(大阪)
• [Presentation] 胎盤について考える (2010)
  • Author(s): 増崎英明
  • Organizer: 第7回香川周産期カンファレンス
  • Place of Presentation: 香川
  • Year and Date: 2010-11-27
• [Presentation] 産科診療から見た生殖医療 (2010)
  • Author(s): 増崎英明
  • Organizer: 第55回日本生殖医学会
  • Place of Presentation: 徳島
• [Presentation] 胎盤由来cell-free mRNA/microRNAの臨床的意義とその応用 (2010)
  • Author(s): 三浦清徳、東島 愛、城 大空、長谷川ゆり、三浦生子、阿部修平、山崎健太郎、増崎英明
  • Organizer: 第18回日本胎盤学会学術集会
  • Place of Presentation: 熊本
• [Presentation] 術中超音波の有用性に関する検討 (2010)
  • Author(s): 吉田 敦、阿部修平、谷川輝美、山崎健太郎、三浦生子、三浦清徳、中山大介、増崎英明
  • Organizer: 第62回日本産科婦人科学会
  • Place of Presentation: 東京
• [Presentation] HELLP症候群と関連した胎盤特異的microRNAの網羅的解析 (2010)
  • Author(s): 三浦清徳、東島愛、三浦生子、阿部修平、山崎健太郎、城大空、長谷川ゆり、中山大介、三嶋博之、木下晃、吉浦孝一郎、増崎英明
  • Organizer: 第55回日本人類遺伝学会
  • Place of Presentation: 埼玉
• [Presentation] A case of mesenchymal diaplasia (2010)
  • Author(s): Miura K, Yamasaki K, Miura S, Nakayama D, Yoshiura K, Nakayama M, Masuzaki H
  • Organizer: 20^<th> ISUOG World Congress
  • Place of Presentation: Prague, Czech Republic
• [Presentation] 腹腔鏡下手術における予防的抗生剤(レボフロキサシン500mg内服)投与に関する検討-レボフロキサシン組織内濃度測定の結果を加えて (2010)
  • Author(s): 三浦清徳, 平木宏一, 北島道夫, 井上統夫, 松脇隆博, 増崎英明
  • Organizer: 第51回 日本産科婦人科内視鏡学会
  • Place of Presentation: 東京
• [Presentation] 新規胎盤特異的microRNAの網羅的スクリーニング (2010)
  • Author(s): 三浦清徳、東島愛、阿部修平、三浦生子、山崎健太郎、嶋田貴子、吉田敦、中山大介、増崎英明
  • Organizer: 第46回日本周産期・新生児医学会
  • Place of Presentation: 神戸
• [Presentation] 妊娠中の子宮頸部細胞診における日母分類、ベセスダシステムおよびHPVスクリーニングの比較 (2010)
  • Author(s): 三浦清徳、山崎健太郎、池本理恵、三浦生子、嶋田貴子、濱口大輔、小寺宏平、藤下晃、鮫島哲郎、村上誠、中山大介、吉浦孝一郎、増崎英明
  • Organizer: 第48回日本婦人科腫瘍学会
  • Place of Presentation: 茨城
• [Presentation] 二卵性一絨毛膜双胎の一例 (2010)
  • Author(s): 三浦清徳、肥後貴史、高橋典子、三浦生子、宮本正史、吉田敦、山崎健太郎、増崎雅子、吉村秀一郎、増崎英明
  • Organizer: 第82回日本超音波医学会
  • Place of Presentation: 京都
• [Presentation] 母体血中胎盤特異的microRNAの分子遺伝学的特徴と妊娠高血圧症候群における流入パターンに関する検討 (2010)
  • Author(s): 三浦清徳、東島愛、阿部修平、三浦生子、山崎健太郎、嶋田貴子、吉田敦、中山大介、増崎英明
  • Organizer: 第62回日本産科婦人科学会
  • Place of Presentation: 東京
• [Book] 前置胎盤・前置癒着胎盤の手術 (2012)
  • Author(s): 増崎英明
  • Total Pages: 153
  • Publisher: メジカルビュー社
• [Book] 画像でみる産科アトラス (2012)
  • Author(s): 増崎英明
  • Total Pages: 218
  • Publisher: メディカ出版
• [Book] 前置胎盤・前置癒着胎盤の手術「前置癒着胎盤 術前評価」 (2012)
  • Author(s): 増﨑英明
  • Total Pages: 13
  • Publisher: メジカルビュー社
• [Remarks]
  • URL: http://www.med.nagasaki-u.ac.jp/gyneclgy/index.html
• [Remarks] 長崎大学産婦人科
  • URL: http://www.med.nagasaki-u.ac.jp/gyneclgy/
• [Remarks]
  • URL: http://www.med.nagasaki-u.ac.jp/gyneclgy/