Analysis of Protamin2deficient mouse

Research Project

Project/Area Number	22591828
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Obstetrics and gynecology
Research Institution	Kumamoto University
Principal Investigator	TAKEDA Naoki 熊本大学, 生命資源研究支援センター, 助教 (90304998)
Project Period (FY)	2010 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	ノックアウトマウス / 発生・分化 / 精子 / ＥＳ細胞 / ジーンターゲティング / ES細胞 / Protamine / 核凝縮 / haploinsufficiency
Research Abstract	Analysis of Protamin2deficient mouseIn the human male infertility, abnormal expression of sperm cell-specific expressionprotein protamine is often seen. Therefore, we've tried to analyze a protamine-2deficient mice by gene targeting method using mouse embryonic cells. Analysis ofprotamine-1 deficient mice to be male sterile in the chimeric mice and heterozygous micein protamine-2 deficient mice was expected.Therefore, by using the gene targeting intoES cells without chromosome Y, to generate mutant mice, we challenged strains ofgerm-deficient mutant mice