Analysis of Protamin2deficient mouse
| Project/Area Number |
22591828
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kumamoto University |
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Principal Investigator |
TAKEDA Naoki 熊本大学, 生命資源研究支援センター, 助教 (90304998)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ノックアウトマウス / 発生・分化 / 精子 / ＥＳ細胞 / ジーンターゲティング / ES細胞 / Protamine / 核凝縮 / haploinsufficiency |
|---|
| Research Abstract |
Analysis of Protamin2deficient mouseIn the human male infertility, abnormal expression of sperm cell-specific expressionprotein protamine is often seen. Therefore, we've tried to analyze a protamine-2deficient mice by gene targeting method using mouse embryonic cells. Analysis ofprotamine-1 deficient mice to be male sterile in the chimeric mice and heterozygous micein protamine-2 deficient mice was expected.Therefore, by using the gene targeting intoES cells without chromosome Y, to generate mutant mice, we challenged strains ofgerm-deficient mutant mice
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Report
(4 results)
Research Products
(2 results)
