| Project/Area Number |
22591830
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATAOKA Hiroaki 宮崎大学, 医学部, 教授 (10214321)
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | 生殖医学 / 胎盤形成 / 受動輸送体遺伝子 / Mfsd2 |
|---|
| Research Abstract |
We made knock out (KO) mice with complete disruption of the Mfsd2gene, which encodes the receptor for the syncytin gene. The weight of KO mice was low, and the growth rate was poor compared with wild type (WT) mice. We performed the pathological comparison of the embryo and placenta on embryonic 18.5 and 12.5 days. There was no difference between KO and WT mice in pathological studies including immunostaining of CD31 and Ki67. Additionally, we used cDNA microarray analysis to compare gene expression profiles between WT and KO mouse embryonic fibroblasts, however, there was no major difference among placenta formation, angiogenesis, cell cycle, or apoptosis.
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