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Development of novel strategy for fertility preservation using cryopreserved ovarian tissue

Research Project

Project/Area Number 22591833
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionSaitama Medical University

Principal Investigator

TAKAI Yasushi  埼玉医科大学, 医学部, 准教授 (60323549)

Co-Investigator(Renkei-kenkyūsha) AKAHORI Taichi  埼玉医科大学, 医学部, 助教 (90573171)
SEKI Hiroyuki  埼玉医科大学, 医学部, 教授 (20179328)
ISHIHARA Osamu  埼玉医科大学, 医学部, 教授 (70176212)
MORITA Ikuo  東京医科歯科大学, 医歯学総合研究科, 教授 (60100129)
SUZUKI Hiroshi  帯広畜産大学, 原虫病センター, 教授 (60333473)
HASHIMOTO Fumie  城西大学, 薬学部, 教授 (10077977)
TOKUDOME Yoshihiro  城西大学, 薬学部, 准教授 (70409390)
Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords生殖医学 / 妊孕性温存 / 卵巣 / 凍結保存 / 幹細胞 / 性同一性障害 / 卵子幹細胞 / 移植 / 生殖幹細胞 / アポトーシス / 血管新生 / 性同-性障害 / 凍結保存法
Research Abstract

A fluorescence-activated cell sorting-based protocol was used with adult human ovarian cortical tissue to purify rare mitotically active cells that have a gene expression profile that is consistent with primitive germ cells. Once established in vitro, these cells (oogonial stem cells; OSCs) can be expanded for months and can spontaneously generate oocytes, as determined by morphology, gene expression and haploid (1n) status.
Injection of the human OSCs into human ovarian cortical biopsies leads to formation of follicles containing oocytes 1-2 weeks after xenotransplantation into immunodeficient female mice. Thus, ovaries of reproductive-age women, similar to adult mice, possess OSCs that can be propagated in vitro as well as generate oocytes in vitro and in vivo.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (46 results)

All 2014 2013 2012 2011 2010

All Journal Article (18 results) (of which Peer Reviewed: 7 results) Presentation (23 results) (of which Invited: 8 results) Book (5 results)

  • [Journal Article] 【幹細胞と生殖医学】卵子幹細胞2014

    • Author(s)
      高井泰
    • Journal Title

      産科と婦人科

      Volume: 81 Pages: 329-335

    • URL

      http://search.jamas.or.jp/link/ui/O226090006

    • Related Report
      2013 Final Research Report
  • [Journal Article] International Committee for Monitoring Assisted Reproductive Technology: world report on assisted reproductive technology2014

    • Author(s)
      Zegers-Hochschild F, Mansour R, Ishihara O(他4名)
    • Journal Title

      Fertil Steril

      Volume: 101 Pages: 366-378

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/24188870

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impact of frozen-thawed single-blastocyst transfer on maternal and neonatal outcome : an analysis of 277,042 single-embryo transfer cycles from 2008 to 2010 in Japan2014

    • Author(s)
      Ishihara O, Araki R, Kuwahara A(他3名)
    • Journal Title

      Fertil Steril

      Volume: 101 Pages: 128-133

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/24268706

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 【幹細胞と生殖医学】 卵子幹細胞2014

    • Author(s)
      高井 泰
    • Journal Title

      産科と婦人科

      Volume: 81 Pages: 329-335

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 卵子幹細胞研究の最近のトピック2013

    • Author(s)
      高井泰
    • Journal Title

      臨床婦人科産科

      Volume: 67 Pages: 172-176

    • NAID

      40019563842

    • URL

      http://search.jamas.or.jp/link/ui/2013077661

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
  • [Journal Article] 【生殖系列細胞の凍結】卵子の凍結保存2013

    • Author(s)
      高井泰
    • Journal Title

      HORMONE FRONTIER IN GYNECOLOGY

      Volume: 20 Pages: 141-147

    • URL

      http://search.jamas.or.jp/link/ui/2013214457

    • Related Report
      2013 Final Research Report
  • [Journal Article] 【生殖系列細胞の凍結】わが国の生殖補助医療における卵子・胚凍結の現状2013

    • Author(s)
      梶原健, 高井泰, 岡垣竜吾(他2名), 田谷順子, 石原理
    • Journal Title

      HORMONE FRONTIER IN GYNECOLOGY

      Volume: 20 Pages: 149-154

    • URL

      http://search.jamas.or.jp/link/ui/2013214458

    • Related Report
      2013 Final Research Report
  • [Journal Article] 【生殖系列細胞の凍結】 卵子の凍結保存2013

    • Author(s)
      高井 泰
    • Journal Title

      HORMONE FRONTIER IN GYNECOLOGY

      Volume: 20 Pages: 141-147

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 【不妊と周産期医療】卵子、卵巣の凍結技術の進歩2012

    • Author(s)
      高井泰,関博之,石原理
    • Journal Title

      周産期医学

      Volume: 42 Pages: 979-983

    • URL

      http://search.jamas.or.jp/link/ui/2012322222

    • Related Report
      2013 Final Research Report
  • [Journal Article] Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women2012

    • Author(s)
      White YA, Woods DC, Takai Y, Ishihara O, Seki H(他1名)L
    • Journal Title

      Nat Med

      Volume: 18 Pages: 413-421

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22366948

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cryopreservation of canine embryos and resulting pregnancies2012

    • Author(s)
      Suzuki H
    • Journal Title

      Reprod Domest Anim 47 Suppl

      Volume: 6 Pages: 141-143

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/23279484

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 【不妊と周産期医療】 卵子、卵巣の凍結技術の進歩2012

    • Author(s)
      高井 泰
    • Journal Title

      周産期医学

      Volume: 42 Pages: 979-983

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Oocyte formation by mitotically active germ cells purified from ovaries of reproductive-age women2012

    • Author(s)
      White, Y.A., Woods, D.C, Takai, Y., Ishihara, O., Seki, H., Tilly, J.L.
    • Journal Title

      Nat Med

      Volume: 18 Issue: 3 Pages: 413-21

    • DOI

      10.1038/nm.2669

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 【卵子のエイジング】生殖医療の将来卵巣凍結2011

    • Author(s)
      高井泰,関博之,石原理
    • Journal Title

      産科と婦人科

      Volume: 78 Pages: 987-991

    • URL

      http://search.jamas.or.jp/link/ui/2011285025

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Journal Article] 【性ステロイドホルモン製剤の使い分け】性ステロイドホルモンの臨床応用性同一性障害と性ステロイド2011

    • Author(s)
      石原理,高橋幸子,梶原健(他2名)
    • Journal Title

      HORMONE FRONTIER IN GYNECOLOGY

      Volume: 18 Pages: 157-162

    • URL

      http://search.jamas.or.jp/link/ui/2011249455

    • Related Report
      2013 Final Research Report
  • [Journal Article] ホルモン/卵巣機能/ヘルスケア性同一性障害(GID : Gender Identity Diorder)2011

    • Author(s)
      石原理,岡垣竜吾,高井泰
    • Journal Title

      産婦人科学レビュー2011

      Pages: 163-166

    • URL

      http://search.jamas.or.jp/link/ui/2011270071

    • Related Report
      2013 Final Research Report
  • [Journal Article] Frozen-thawed blastocyst transfer reduces ectopic pregnancy risk : an analysis of single embryo transfer cycles in Japan2011

    • Author(s)
      Ishihara O, Kuwahara A, Saitoh H
    • Journal Title

      Fertil Steril

      Volume: 95 Pages: 1966-1969

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21377154

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Implantation of capillary structure engineered by optical lithography improves hind limb ischemia in mice2010

    • Author(s)
      Akahori T, Kobayashi A, Komaki M(他6名)
    • Journal Title

      Tissue Eng Part A

      Volume: 16 Pages: 953-959

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/19947885

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] がん・生殖医療のこれからのために2014

    • Author(s)
      高井泰
    • Organizer
      岐阜県がん・生殖医療ネットワークGPOFs 2014ミーティング
    • Place of Presentation
      岐阜
    • Year and Date
      2014-02-14
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] 女性血液疾患患者の妊孕性温存1 -卵子凍結、胚凍結2014

    • Author(s)
      高井泰
    • Organizer
      がんと生殖に関するシンポジウム2014−血液疾患患者さんの妊孕性温存対策のこれからを考える−
    • Place of Presentation
      東京
    • Year and Date
      2014-02-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 女性血液疾患患者の妊孕性温存1 - 卵子凍結、胚凍結2014

    • Author(s)
      高井 泰
    • Organizer
      がんと生殖に関するシンポジウム2014ー血液疾患患者さんの妊孕性温存対策のこれからを考えるー
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 卵子老化と生殖医療2013

    • Author(s)
      高井泰
    • Organizer
      第30回埼玉県母性衛生学会総会・学術講演会
    • Place of Presentation
      さいたま
    • Year and Date
      2013-11-30
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Presentation] Recent Progress and New Trends of ART in Japan - A brief review for oogonial stem cells in human ovary.2013

    • Author(s)
      Takai Y
    • Organizer
      The International Federation of Fertility Societies 21st World Congress on Fertility and Sterility Regional Meeting
    • Place of Presentation
      Boston, MA
    • Year and Date
      2013-10-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 乳がん患者に対する妊孕性温存〜がん生殖医療update〜2013

    • Author(s)
      高井泰
    • Organizer
      12th Kyushu Brest Cancer Workshop〜ホルモン療法の基礎と臨床〜
    • Place of Presentation
      福岡
    • Year and Date
      2013-07-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 卵子幹細胞研究の現状と未来2013

    • Author(s)
      高井泰
    • Organizer
      第125回関東連合産科婦人科学会総会・学術集会
    • Year and Date
      2013-06-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 妊孕性温存療法の提言その1—卵子凍結、胚凍結2013

    • Author(s)
      高井泰
    • Organizer
      がんと生殖に関するシンポジウム2013—妊孕性温存の診療を考える—
    • Place of Presentation
      東京
    • Year and Date
      2013-04-21
    • Related Report
      2013 Final Research Report
  • [Presentation] 最新情報提供セッション「生殖医療・生殖医学研究とGIDのたいせつな関係」GIDと卵子幹細胞2013

    • Author(s)
      高井泰
    • Organizer
      最新情報提供セッション「生殖医療・生殖医学研究とGIDのたいせつな関係」GIDと卵子幹細胞
    • Place of Presentation
      さいたま
    • Related Report
      2013 Final Research Report
  • [Presentation] 妊孕性温存療法の提言その1ー卵子凍結、胚凍結2013

    • Author(s)
      高井 泰
    • Organizer
      がんと生殖に関するシンポジウム2013―妊孕性温存の診療を考える―
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 卵子幹細胞研究の現状と未来2013

    • Author(s)
      高井 泰
    • Organizer
      第125回関東連合産科婦人科学会総会・学術集会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 乳がん患者に対する妊孕性温存~がん生殖医療update~2013

    • Author(s)
      高井 泰
    • Organizer
      12th Kyushu Brest Cancer Workshop~ホルモン療法の基礎と臨床~
    • Place of Presentation
      福岡
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] A brief review for oogonial stem cells in human ovary2013

    • Author(s)
      Takai Y
    • Organizer
      The International Federation of Fertility Societies 21st World Congress on Fertility and Sterility
    • Place of Presentation
      Boston, USA
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 最新情報提供セッション「生殖医療・生殖医学研究とGIDのたいせつな関係」GIDと卵子幹細胞2013

    • Author(s)
      高井 泰
    • Organizer
      GID(性同一性障害)学会第15回研究大会
    • Place of Presentation
      さいたま 大宮ソニックシティ
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 生殖医療と遺伝カウンセリング最近の諸問題卵子凍結、卵子提供、卵子幹細胞2012

    • Author(s)
      高井泰
    • Organizer
      第57回日本生殖医学会学術講演会
    • Place of Presentation
      長崎
    • Related Report
      2013 Final Research Report
  • [Presentation] シンポジウム「Oncofertility:生殖医療と腫瘍科学の融合」がん・生殖医療における生殖補助医療の現状と課題2012

    • Author(s)
      高井泰
    • Organizer
      第30回日本受精着床学会学術講演会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] シンポジウム「Oncofertility:生殖医療と腫瘍科学の融合」がん・生殖医療における生殖補助医療の現状と課題2012

    • Author(s)
      高井 泰
    • Organizer
      第30回日本受精着床学会学術講演会
    • Place of Presentation
      大阪 グランキューブ大阪
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 生殖医療と遺伝カウンセリング 最近の諸問題 卵子凍結、卵子提供、卵子幹細胞2012

    • Author(s)
      高井 泰
    • Organizer
      第57回日本生殖医学会学術講演会
    • Place of Presentation
      長崎 長崎ブリックホール
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ヒト卵巣組織のガラス化凍結保存とマウスへの異種移植−臨床応用に向けて2010

    • Author(s)
      高井泰,林直樹,大久保貴司(他5名)
    • Organizer
      第62回日本産科婦人科学会総会・学術講演会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト卵巣組織のガラス化凍結保存とマウスへの異種移植-臨床応用に向けて2010

    • Author(s)
      高井泰
    • Organizer
      第62回日本産科婦人科学会総会・学術講演会
    • Place of Presentation
      東京
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト卵巣組織のガラス化凍結保存とマウスへの異種移植-臨床応用に向けて2010

    • Author(s)
      高井泰
    • Organizer
      第28回日本受精着床学会総会・学術講演会
    • Place of Presentation
      横浜
    • Related Report
      2010 Annual Research Report
  • [Presentation] Vitrification and xenografting of human ovarian tissue2010

    • Author(s)
      Takai, Y.
    • Organizer
      IFFS 20th World Congress on Fertility & Sterility
    • Place of Presentation
      ミュンヘン
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒト卵巣組織のガラス化凍結保存とマウスへの異種移植-臨床応用に向けて2010

    • Author(s)
      高井泰
    • Organizer
      第55回日本生殖医学会総会・学術講演会
    • Place of Presentation
      徳島
    • Related Report
      2010 Annual Research Report
  • [Book] 胚凍結.がん・生殖医療−妊孕性温存の診療2013

    • Author(s)
      高井泰, (鈴木直,竹原祐編)
    • Publisher
      医歯薬出版, (東京)
    • Related Report
      2013 Final Research Report
  • [Book] 絨毛検査、羊水検査、臍帯穿刺、児頭採血、母体血による胎児診断, (目でみる妊娠と出産)2013

    • Author(s)
      高井泰
    • Publisher
      文光堂, (東京)
    • Related Report
      2013 Final Research Report
  • [Book] 卵子幹細胞.卵巣組織の凍結・移植—新しい妊孕性温存療法の実践2013

    • Author(s)
      高井泰, (鈴木直編)
    • Publisher
      医歯薬出版, (東京)
    • Related Report
      2013 Final Research Report
  • [Book] がん細胞の再移入に関して.卵巣組織の凍結・移植—新しい妊孕性温存療法の実践2013

    • Author(s)
      高井泰, (鈴木直編)
    • Publisher
      医歯薬出版, (東京)
    • Related Report
      2013 Final Research Report
  • [Book] 生命の誕生に向けて第2版-ヒト生殖腺の凍結保存2011

    • Author(s)
      高井泰
    • Publisher
      近代出版
    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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