Enhanced safety of infertile therapy by means of quality control of sperm - relationship between vacuole in sperm head and DNA fragmentation
| Project/Area Number |
22591837
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
KANEKO Satoru 東京歯科大学, 歯学部, 講師 (40214457)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 品質管理 / 精子頭部空胞 / DNA 損傷 / 顕微授精 / 染色体 / DNA損傷 / ヒト精子 / DNA / 電気泳動 / DNA断片化 / DNA fiber |
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| Research Abstract |
The early stage of DNA fragmentation in a single nucleus could be observed by single cell pulse field gel electrophoresis (SCPFGE). First, a few large fibrous fragments were derived from a bundle of long chain fibers, the cleavages were advanced until finally almost all the DNA was shredded to granular fragments. There found no significant correlation between DNA fragmentation and vacuole in sperm head. The cell culture under high oxygen concentration caused increment of the percentage of DNA fragmentation.
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Report
(4 results)
Research Products
(3 results)
