The synthesis and function of melatonin in the ovary

• Project/Area Number: 22591839
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ITOH Masanori 東京医科歯科大学, 教養部, 教授 (30257349)
• Project Period (FY): 2010-10-20 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: メラトニン / セロトニン / 卵巣 / 卵母細胞 / ラット / メラトニン合成酵素

Research Abstract

Melatonin is originally identified in the pineal gland, where it is synthesized enzymatically from serotonin by the sequential action of arylalkylamine N-acetyltransferase (AANAT) and acetylserotonin O-methyltransferase (ASMT). Our data revealed that AANAT and ASMT are expressed in the adult rat ovary. like the pineal gland. AANAT protein is localized to the oocyte, corpus luteum, and medulla including mast cells. AANAT protein was found in oocytes at all stages of follicular development, and its levels in oocytes increased progressively throughout the follicular development. Furthermore, we showed that melatonin is synthesized from serotonin in oocytes. Melatonin synthesized in the oocyte may be implicated in its own growth and/or maturation, for example, by acting as a calmodulin antagonist and/or an antioxidant.

Research Products

• [Journal Article] Effects of 5 alpha-dihydrotestosterone and 17 beta-estradiol on the mouse ovarian follicle development and oocyte maturation (2014)
  • Author(s): Tarumi, W., Itoh, M.T., Suzuki, N
  • Journal Title: Plos One Volume: 9
  • Peer Reviewed
• [Journal Article] The rat oocyte synthesizes melatonin (2013)
  • Author(s): Sakaguchi, K., Itoh, M.T., Takahashi, N., Tarumi, W., Ishizuka, B
  • Journal Title: Reprod. Fertile. Dev Volume: 25 Issue: 4 Pages: 674-682
  • DOI: 10.1071/rd12091
  • Peer Reviewed
• [Journal Article] Androstenedione inducees abnormalities in morphology and function of developimg oocytes, which impairs oocyte meiotic competence (2012)
  • Author(s): Tarumi, W., Tsukamoto, S., Okutsu, Y., Takahashi, N., Horiuchi, T., Itoh, M.T., Ishizula, B
  • Journal Title: Fertil. Steril Volume: 97 Issue: 2 Pages: 469-476
  • DOI: 10.1016/j.fertnstert.2011.11.040
  • Peer Reviewed
• [Journal Article] Number of CGG repeats in the FMR1 gene of Japanease patients with primary ovarian in insufficiency (2011)
  • Author(s): Ishizuka, B., Okamoto, N., Hamada, N., Sugishita, Y., Saito, J., Takahashi, N., Ogata, T., Itoh, M
  • Journal Title: T. Fertile. Steril Volume: 96 Pages: 1170-1174
  • Peer Reviewed
• [Journal Article] Gonadotopins up-regulate the expression of enolase 2, but not, (細胞の紡錘体形成異常を誘起する) (2011)
  • Author(s): takahashi, N., Tarumi, W., Itoh, M.T., Ishizuka, B., Yoshioka, N.
  • Journal Title: Endocr. J Volume: 58 Pages: 941-948
  • Peer Reviewed
• [Journal Article] Gonadotropins up-regulate the expression of enolase 2, but not enolase 1, in the rat ovary (2011)
  • Author(s): Yoshioka, N.
  • Journal Title: Endocrine Journal Volume: 58 Issue: 11 Pages: 941-948
  • DOI: 10.1507/endocrj.EJ11-0098
  • NAID: 10030392136
  • ISSN: 0918-8959, 1348-4540
  • Peer Reviewed
• [Journal Article] Number of CGG repeats in the FMR! gene of Japanese patients with primary ovarian insufficiency (2011)
  • Author(s): Ishizuka, B.
  • Journal Title: Fertility and Sterility Volume: 96 Pages: 1170-1174
  • Peer Reviewed
• [Presentation] -iminodipropionitrile (IDPN)の生殖軸に対する毒性 (2013)
  • Author(s): 高橋則行、樽見航、浜田直美、伊藤正則、河村和弘、田中守、鈴木直、石塚文平
  • Organizer: 第58回日本生殖医学会総会・学術講演会
  • Place of Presentation: 神戸
  • Year and Date: 2013-03-03
• [Presentation] Effects of 5α-dihydoroxytestosterone and 17-βestradiol on the ovarian follicles (2013)
  • Author(s): Tarumi, W., Itoh,M.T., Suzuki, N., Ishizuka, B
  • Organizer: The 9th Conference of the Pacific Rim Society for Fertility and Sterility-Cutting-edge Advances in Reproductive Medicine
  • Place of Presentation: 神戸
• [Presentation] Effects of 5α-dihydroxytestosterone and 17β-estradiol on the ovarian follicles (2013)
  • Author(s): Tarumi W, Tsukamoto S, Okutsu Y, Takahashi N, Horiuchi T, Itoh MT, Ishizuka B.
  • Organizer: The 9th Conference of the Pacific Rim Society for Fertility and Sterility-Cutting-edge Advances in Reproductive Medicine
  • Place of Presentation: 神戸
• [Presentation] 3,3'-iminodipropionitrile (IDPN)の生殖軸に対する毒性 (2013)
  • Author(s): 高橋則行、樽見航、浜田直美、伊藤正則、河村和弘、田中守、鈴木直、石塚文平
  • Organizer: 第58回日本生殖医学会総会・学術講演会
  • Place of Presentation: 神戸
• [Presentation] 卵成熟/卵変性とNeurofilament-Hのリン酸化との関連 (2012)
  • Author(s): 高橋則行、河村和弘、樽見航、浜田直美、伊藤正則、田中守、鈴木直、石塚文平
  • Organizer: 第17回日本生殖内分泌学術集会
  • Place of Presentation: 東京
• [Presentation] アンドロゲンは卵Kava suspension prepared from roots of Piper methysticum on behaviors in mice (2012)
  • Author(s): 樽見航、伊藤正則、河村和弘、石塚文平、田中守、鈴木直
  • Organizer: International Conference on Chemistry, Environment and Climate Change 2011
  • Place of Presentation: フィジー共和国スヴァ
• [Presentation] ウズラにおけるメラトニンの血糖低下作用 (2012)
  • Author(s): 和田友里子、山下萌、丸山雄介、伊藤正則、服部淳彦
  • Organizer: 第37回日本比較内分泌学会大会
  • Place of Presentation: 福井
• [Presentation] ラット卵で発現するNeurofilament Hは卵の変性および成熟時にリン酸化される (2012)
  • Author(s): 高橋則行、樽見航、杉下陽堂、浜田直美、伊藤正則、河村和弘、田中守、鈴木直、石塚文平
  • Organizer: 第57回日本生殖医学会総会・学術講演会
  • Place of Presentation: 長崎
• [Presentation] アンドロステンジオンによる卵成熟阻害作用:単一卵胞培養系を用いた解析 (2012)
  • Author(s): 樽見航、伊藤正則、河村和弘、石塚文平、田中守、鈴木直
  • Organizer: 第57回日本生殖医学会総会・学術講演会
  • Place of Presentation: 長崎
• [Presentation] 卵発育・成熟に対するアンドロステンジオンの影響:単一卵胞培養系による検討 (2012)
  • Author(s): 樽見航、伊藤正則、河村和弘、洞下由記、塚本早苗、石塚文平、田中守、鈴木直
  • Organizer: 生殖バイオロジー東京シンポジウム2012
  • Place of Presentation: 東京
• [Presentation] Androstenedione induces abnormalities in morphology and function of developing oocytes, which impairs oocyte meiotic competence (2012)
  • Author(s): Tarumi, W., Tsukamoto, S., Okutsu, Y., Takahashi, N., Horiuchi, T., Itoh, M.T., Ishizuka, B
  • Organizer: The International Ovarian Conference 2012
  • Place of Presentation: 東京
• [Presentation] 二つの研究-卵胞の発育・成熟機構と本能行動の脳制御機構 (2011)
  • Author(s): 伊藤正則
  • Organizer: 第56回日本生殖医学会総会・学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-05
• [Presentation] Androstenedione induces abnormalities in morphology and function of developing oocytes, which impaires oocyte meiotic competence (2011)
  • Author(s): Tarumi, W.
  • Organizer: 16^<th> World Congress on In Vitro Fertilization/5^<th> World Congress on In Vitro Maturation
  • Place of Presentation: 東京
  • Year and Date: 2011-09-10
• [Presentation] Day 3 thymectomy mouseを用いた抗卵巣自己抗体の同定 (2011)
  • Author(s): 岡本直樹、寺山隼人、石塚文平、平井宗一、内藤宗和、高橋則行、伊藤正則、伊藤正裕
  • Organizer: 第26回日本生殖免疫学会・学術集会
  • Place of Presentation: 名古屋
• [Presentation] ラット卵巣で発現するエノラーゼ2 (2011)
  • Author(s): 高橋則行、吉岡伸人、坂口健一郎、樽見航、伊藤正則、石塚文平
  • Organizer: 第56回日本生殖医学会総会・学術講演会
  • Place of Presentation: 横浜
• [Presentation] Effects of Kava suspension prepared from roots of Piper methysticum on behaviors in mice (2011)
  • Author(s): Murakami, S., Satou, T., Niihara, K, Itoh, M.T., Yoshida, M., Voro, T., Prasad, S
  • Organizer: International Conference on Chemistry, Environment and Climate Change 2011
  • Place of Presentation: フィジー共和国スヴァ
• [Presentation] 6種ラベンダー精油と情動行動の関係 (2011)
  • Author(s): 高橋瑞穂、石橋拓也、宮川三千世、佐藤忠章、定本清美、小池一男、伊藤正則、村上志緒、林真一郎
  • Organizer: 日本生薬学会第58回年会
  • Place of Presentation: 東京
• [Presentation] Andorostenedione induces abnormalities in morphology and function of developing oocytes, which impairs oocyte meiotic competence (2011)
  • Author(s): Tarumi, W., Tsukamoto, S., Okutsu, Y., Takahashi, N., Horiuchi, T., Itoh, M.T., Ishizuka, B.
  • Organizer: 16 th World Congress on In Vitro Fertilization/5^<th> World Congress on In Vitro Maturation
  • Place of Presentation: 仙台
• [Presentation] Day 3 thymectomy mouseを用いた自己免疫性POF原因因子の検討 (2011)
  • Author(s): 岡本直樹、寺山隼人、平井宗一、内藤宗和、高橋則行、伊藤正則、伊藤正裕、石塚文平
  • Organizer: 第29回日本受精着床学会総会・学術講演会
  • Place of Presentation: 東京
• [Presentation] 新生仔期の胸腺除去マウスにおける卵巣機能に影響を及ぼす自己免疫因子 (2011)
  • Author(s): 岡本直樹、高橋則行、伊藤正則、石塚文平
  • Organizer: 第63回日本産科婦人科学会学術集会
  • Place of Presentation: 大阪
• [Presentation] メラトニンが卵巣機能アンチエイジング因子である可能性 (2010)
  • Author(s): 伊藤正則
  • Organizer: 第28回日本受精着床学会・学術講演会
  • Place of Presentation: 横浜
  • Year and Date: 2010-07-28
• [Presentation] 思春期前ラット卵胞のゴナドトロピン感受性獲得時期 (2010)
  • Author(s): 高橋則行、伊藤正則、石塚文平
  • Organizer: 第62回日本産科婦人科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-15
• [Presentation] 卵胞の発育・成熟に対するandrostenedioneの影響-単一卵胞培養系での検討 (2010)
  • Author(s): 樽見航、塚本早苗、奥津由記、石山めぐみ、田村みどり、堀内俊孝、伊藤正則、石塚文平
  • Organizer: 第55回日本生殖医学会総会・学術講演会
  • Place of Presentation: 徳島
• [Presentation] Experimental autoimmune factor affects ovarian function after neonatal thymectomy in the mouse (2010)
  • Author(s): Okamoto, N., Takahashi, N., Itoh, M.T., Ishizuka, B.
  • Organizer: FertiLink 2010/International Ovarian Conference 2010
  • Place of Presentation: 京都
• [Presentation] 繁殖期の雌キンギョにおけるカルシウム代謝に対するメラトニンの作用 (2010)
  • Author(s): 丸山雄介、鈴木信雄、伊藤正則、服部淳彦
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
• [Presentation] Nestinの発現は卵胞の状態により変動する (2010)
  • Author(s): 高橋則行、伊藤正則、石塚文平
  • Organizer: 第28回日本受精着床学会総会・学術講演会
  • Place of Presentation: 横浜
• [Book] (2011)
  • Author(s): 石塚文平, (鈴木秋悦編)
  • Publisher: 医歯薬出版
• [Book] 生殖卵巣学-臨床への進展 (2011)
  • Author(s): 伊藤正則
  • Publisher: 医歯薬出版