| Project/Area Number |
22591854
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Osaka University |
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Principal Investigator |
ENOMOTO Takayuki 大阪大学, 大学院・医学系研究科, 招へい教授 (90283754)
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| Co-Investigator(Kenkyū-buntansha) |
FUJITA Masami 大阪大学, 大学院・医学系研究科, 講師 (60303963)
YOSHINO Kiyoshi 大阪大学, 大学院・医学系研究科, 講師 (90362730)
UEDA Yutaka 大阪大学, 大学院・医学系研究科, 助教 (10346215)
KIMURA Toshihiro 大阪大学, 大学院・医学系研究科, 助教 (90584524)
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 婦人科腫瘍学 / 子宮体癌 / 卵巣癌 / AnnexinA4 / 蛋白質 / トランスポーター |
|---|
| Research Abstract |
In this report, we investigated the mechanism of platinum resistance induced by Anx A4 in endometrial carcinoma cells (HEC1 cells) which had low level expression of Anx A4. In HEC1 cells, forced expression of Anx A4 resulted in chemoresistance to Cisplatin or Carboplatin in vitro and in vivo. Most importantly, after exposure to Cisplatin or Carboplatin, Anx A4 changed localization from the cytoplasm to the cellular membrane and Anx A4 and ATP7A co-localized to the cellular membrane. Our results indicate that enhanced expression of Anx A4 confers platinum resistance by promoting efflux of platinum drugs via ATP7A.
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