Analysis of oncogenes involved in invasion or metastasis of endometrial cancer and detection of endometrial cancer stem cells

• Project/Area Number: 22591855
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Osaka University
• Principal Investigator: FUJITA Masami 大阪大学, 大学院・医学系研究科, 講師 (60303963)
• Co-Investigator(Kenkyū-buntansha): ENOMOTO Takayuki 大阪大学, 大学院・医学系研究科, 招聘教授 (90283754) ; YOSHINO Kiyoshi 大阪大学, 大学院・医学系研究科, 講師 (90362730) ; UEDA Yutaka 大阪大学, 大学院・医学系研究科, 助教 (10346215) ; KIMURA Toshihiro 大阪大学, 大学院・医学系研究科, 講師 (90584524)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 婦人科腫瘍学 / 子宮内膜癌 / 浸潤 / 転移 / 癌幹細胞 / CTOS / 化学療法 / CIOS

Research Abstract

Analysis of the expression of the TSC403 genes using clinical specimens was done. T ended high degree of expression was observed in endometrial cancer tissue. There was no gene amplification.Invasion analysis of cancer cells in vitro using the TSC403 gene expressing cell line was done. Significant correlation was found.From endometrial cancer tissue specimens and normal endometrial tissue samples, using the iTRAQ technique, we identified proteins whose expression has been attenuated or e nhanced. Protein X is related to anti-cancer drug sensitivity .The analysis of cancer stem cells was done by FACS flow cytometry using CD133 as a stem cell surface marker. Primary culture of cancer stem cells was done, but not continued.From endometrial cancer tissue, new cancer cells adjusting method CTOS: cancer tissueoriginated spheroid was tried. It succeeds in creating cultured cells (CTOS) in endometrial cancer. CTOS was created, could be performed vitro drug sensitivity test. This study, may contribute to the development of a new treatment system of endometrial cancer.

Research Products

• [Journal Article] Plasma nlembrane proteonlics identifies bone marrow stromal antigen 2 as a potential therapeutic target in endometrial cancer (2013)
  • Author(s): Yokoyama T, Enomoto T, Serada S, Morimoto A, Matsuzaki S, Ueda Y, Yoshino K, Fujita M, Kyo S, Iwahori K, Fujimoto M, Kimura T, Naka T
  • Journal Title: Int J Cancer Volume: 132(2) Issue: 2 Pages: 472-84
  • DOI: 10.1002/ijc.27679
  • Peer Reviewed
• [Journal Article] CRABP1-reduced expression is associated with poorer prognosis in serous and clear cell ovarian adenocarcinoma (2012)
  • Author(s): Miyake T, Ueda Y, Matsuzaki S, Miyatake T, Yoshino K, Fujita M, Nomura T, Enomoto T,Kimura T
  • Journal Title: Cancer Res Clin Oncol Volume: 137 Issue: 4 Pages: 715-722
  • DOI: 10.1007/s00432-010-0930-8
  • Peer Reviewed
• [Journal Article] nvestigating the relative efficacies of combination chemotherapy of paclitaxel/carboplatin, with or without anthracycline, for endometrial carcinoma (2012)
  • Author(s): Fujiwara K, Egawa-Takata T, Ueda Y, Kimura T, Yoshino K, Fujita M, Miyatake T, Kamiura S, Enomoto T, Kimura T
  • Journal Title: Arch Gyn Ob Volume: 285 Issue: 5 Pages: 1447-53
  • DOI: 10.1007/s00404-011-2154-9
  • Peer Reviewed
• [Journal Article] Chemotherapy for endometrial carcinoma (GOGO-EM1study):TEC(paclitaxel, epirubicin and carboplatin) is an effective remission-induction and adjuvant therapy (2011)
  • Author(s): Egawa-Takata T, Ueda Y, Fujita M, Yoshino K, Enomoto T
  • Journal Title: Cancer Chemother Pharmacol Volume: 68 Issue: 6 Pages: 1603-10
  • DOI: 10.1007/s00280-011-1638-4
  • Peer Reviewed
• [Presentation] Annexin A4-conferred platinum resistance is mediated by the copper transporter ATP7A (2013)
  • Author(s): Matsuzaki S, Morimoto A, Serada S, Yokoyama T , Kimura T, Kobayashi E, Ueda Y ,Yoshino K, Fujita M, Enomoto T , Naka T, Kimura T
  • Organizer: American Association for Cancer Research Annual meeting
  • Place of Presentation: Washington DC
  • Year and Date: 2013-04-10
• [Presentation] Chemo-sensitivity test using cancer-tissue originated spheroids(CTOSs) of endometrial cancer (2012)
  • Author(s): Kiyohara Y , Kimura T, Yoshino K,Morimoto A, Ueda Y , Fujita M, Inoue M,Enomoto T, Kimura T
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 2012-09-21
• [Presentation] Establishment of cancer-tissue originated spheroids (CTOSs) and chemo-sensitivity test for endometrial cancer (2012)
  • Author(s): Kimura T, Yoshino K, Hiramatsu K, Kiyohara Y , Morimoto A, Yokoyama T , Kobayashi E, Ueda Y , Fujita M, Inoue M,Enomoto T , Kimura T
  • Organizer: American Association for Cancer Research Annual meeting
  • Place of Presentation: Chicago
  • Year and Date: 2012-03-03
• [Presentation] 子宮体癌に対するTEC療法(paclitaxel + epirubicin + carboplatin)の治療成績 (2011)
  • Author(s): 高田友美、上田豊、三宅貴仁、宮武崇、藤田征巳、 吉野潔、岡澤美佳、筒井建紀、森重健一郎、木村正、山嵜正人、西崎孝道、長松正章、伊藤公彦、浅田昌宏、荻田和秀、脇本昭憲、山本敏也、西尾幸浩、榎本隆之
  • Organizer: 日本婦人科腫瘍学会学術講演会
  • Place of Presentation: 札幌
  • Year and Date: 2011-07-23
• [Presentation] 子宮体癌に対するTEC療法(paclitaxel+epirubicin+carboplatin)の治療成績 (2011)
  • Author(s): 高田友美、上田豊、三宅貴仁、宮武崇、藤田征巳、吉野潔、岡澤美佳、筒井建紀、森重健一郎、木村正、山嵜正人、西崎孝道、長松正章、伊藤公彦、浅田昌宏、荻田和秀、脇本昭憲、山本敏也、西尾幸浩、榎本隆之
  • Organizer: 日本婦人科腫瘍学会学
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-07-23