Elucidating a determinant of development as a novel therapeutic target of ovarian clear cell adenocarcinoma

• Project/Area Number: 22591860
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Yokohama City University
• Principal Investigator: MIYAGI Etsuko 横浜市立大学, 附属病院, 准教授 (40275053)
• Co-Investigator(Kenkyū-buntansha): HIRAHARA Fumiki 横浜市立大学, 医学研究科, 教授 (30201734)
• Co-Investigator(Renkei-kenkyūsha): MIYAGI Yohei 地方独立行政法人神奈川県立病院機構神奈川県立がんセンター(臨床研究所), がん分子病態学部, 総括部長 (00254194)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 婦人科腫瘍学 / 卵巣癌 / 卵巣明細胞腺癌 / アネキシンA4 / 浸潤 / 増殖 / 薬剤耐性 / 転移 / 明細胞腺癌

Research Abstract

The annexin A4 (ANXA4), one of the highly expressed proteins in ovarian clear cell adenocarcinoma (OCCA), which is typically chemo-resistant and poor-prognosis disease, was our research target. Two OCCA cell lines in which ANXA4 expression is suppressed by shRNA were used. Significantly restricted proliferation and increased chemo-sensitivity were recognized in an acidic-ANXA4-expressing cell, whereas decreased invasiveness was detected in a basic-ANXA4-expressing cell.

Research Products

• [Journal Article] HIF2α-Sp1 interaction mediates a deacetylation-dependent FVII-gene activation under hypoxic conditions in ovarian cancer cells (2012)
  • Author(s): Koizume S, Ito S, Miyagi E, Hirahara F, Nakamura Y, Sakuma Y, Osaka H, Takano Y, Ruf W, Miyagi Y
  • Journal Title: Nucleic Acid Research Volume: 40(12) Issue: 12 Pages: 5389-5401
  • DOI: 10.1093/nar/gks201
  • Peer Reviewed
• [Journal Article] Japanese Research Group for Development of Cervical Cancer Screening Guidelines : The Japanese guideline for cervical cancer screening (2010)
  • Author(s): Hamashima C
  • Journal Title: Jpn J Clin Oncol Volume: 40 Pages: 485-502
  • Peer Reviewed
• [Journal Article] 子宮体部原発扁平上皮癌の1例 (2010)
  • Author(s): 長谷川哲哉
  • Journal Title: 日本臨床細胞学会神奈川県支部会誌 Volume: 15 Pages: 65-70
  • Peer Reviewed
• [Journal Article] JEB(carboplatin,etoposide,bleomycin)療法を施行した卵巣悪性胚細胞腫瘍の4例 (2010)
  • Author(s): 横須賀とも子
  • Journal Title: 小児がん Volume: 47 Pages: 111-115
  • Peer Reviewed
• [Presentation] Functional analysis of annexin A4 and the implication in ovarian clear cell adenocarcinoma (OCCA) phenotypes (2012)
  • Author(s): Mogami T, Yokota N, Yoshihara M, Nakamura Y, Sakuma Y, Koizume S, Asai-Sato M, Miyagi E, Hirahara F, Miyagi Y
  • Organizer: American Association for Cancer Research Annual Meeting
  • Place of Presentation: Chicago, USA.
  • Year and Date: 2012-04-15
• [Presentation] 卵巣明細胞腺癌で高発現する annexin A4 の機能解析 (2012)
  • Author(s): 最上多恵,横田奈朋,佐藤美紀子,沼崎令子,宮城悦子,平原史樹
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸ポートピアホテル、神戸国際展示場(兵庫県)
  • Year and Date: 2012-04-14
• [Presentation] Functional analysis of annexin A4 and the implication in ovarian clear cell adenocarcinoma (OCCA) phenotypes (2012)
  • Author(s): Mogami T, Yokota N, Yoshihara M, Nakamura Y, Sakuma Y, Koizume S, Asai-Sato M, Miyagi E, Hirahara F, Miyagi Y
  • Organizer: The 3rdJohnsHopkins Ovarian Cancer Symposium
  • Place of Presentation: Baltimore, USA.
• [Presentation] Functional analysis of annexin A4 and the implication in ovarian clear cell adenocarcinoma (OCCA) phenotypes (2012)
  • Author(s): Mogami T
  • Organizer: American Association for Cancer Research Annual Meeting
  • Place of Presentation: Chicago, USA
• [Presentation] 卵巣明細胞腺癌で高発現するannexin A4の機能解析 (2012)
  • Author(s): 最上多恵
  • Organizer: 第64回日本産科婦人科学会学術講演会
  • Place of Presentation: 神戸ポートピアホテル・神戸国際展示場(兵庫県)
• [Presentation] Functional analysis of annexinA4 and the implication in ovarian clear cell adenocarcinoma (OCCA) phenotypes (2011)
  • Author(s): 最上多恵
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2011-10-04
• [Presentation] 卵巣明細胞腺癌における ANXA4 発現の解析 (2011)
  • Author(s): 最上多恵,横田奈朋,常住 淳,辻祥太郎,小井詰史郎,佐藤美紀子,宮城悦子,宮城洋平,平原史樹
  • Organizer: 第10回日本婦人科がん分子標的研究会学術集会
  • Place of Presentation: 松江しんじ湖温泉ホテル(島根県)
  • Year and Date: 2011-07-02
• [Presentation] 卵巣明細胞腺癌におけるANXA4発現の解析 (2011)
  • Author(s): 最上多恵
  • Organizer: 第10回日本婦人科がん分子標的研究会学術集会
  • Place of Presentation: 松江しんじ湖温泉ホテル一畑(島根県)
  • Year and Date: 2011-07-02
• [Book] よくわかる最新医学子宮がん (2010)
  • Author(s): 監修:宮城悦子
  • Total Pages: 150
  • Publisher: 主婦の友社