| Project/Area Number |
22591947
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KINOSHITA Shigeru 京都府立医科大学, 医学研究科, 教授 (30116024)
SHINOMIYA Katsuhiko 京都府立医科大学, 医学研究科, 助教 (50585289)
NOGUTHI Atsushi 京都府立医科大学, 医学研究科, 助教 (00515992)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 角膜内皮 / 原始繊毛 / 角膜移植 / 角膜保存液 / 組織学的検査 / 非健常細胞 / 組織学的検討 / 不死化ウサギ角膜内皮細胞 / 死細胞 |
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| Research Abstract |
We examined corneal endothelia of donor eyes which were provided by the U.S. Eye-bank and were used for corneal transplantation, and we found that about 5% of dead cells were present in the endothelial cells. It was considered that the increase of dead cells during storage might cause decrease of the number of the endothelial cells aftercorneal transplantation. In addition, when the rabbit cornea was stored at 4 ℃ in the corneal storage media, primary cilia in the corneal endothelial cells were disappeared, and they were reappeared after recovery culture at 37 ℃. The presence of primary cilia was thought to have some kind of relationship to viability of corneal endothelium.
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