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vascular invasion and cultivated oral mucosal epithelial sheets

Research Project

Project/Area Number 22591951
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Ophthalmology
Research InstitutionTokyo Dental College

Principal Investigator

SATAKE Yoshiyuki  東京歯科大学, 歯学部, 講師 (60385143)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords移植・再生医療 / 眼表面再建 / 血管新生
Research Abstract

Transplantation of cultivated oral mucosal epithelial sheets is one of the surgical procedures to reconstruct ocular surface in patients with limbal stem cell deficiency. The major advantage of this treatment is the absence of the need for postoperative immunosuppressive therapy. There are two types of cultivated oral mucosal epithelial sheets with or without amniotic membrane as a substrate. Since vascular invasion underneath transplanted sheet, which might cause decreased visual acuities were common in cases of the transplantation of cultivated oral mucosal epithelium sheets with a substrate during postoperative courses, this finding was considered a possibility of the existence substrate is involved in the pathogenesis. First, I examined the relationship between the existence of substrate and the expression of angiogeneic growth factors and angiogenesis inhibitors in cultivated oral mucosal epithelial sheets. There was no difference of the expression of these factors at both transcr … More iption and protein level. Next, the histopathological examination of corneal tissue harvested at the time of secondary keratoplasty following the transplantation of cultivated oral mucosal epithelial transplantation, and examined was performed. Each pathological finding had strong influence of microenvironment of ocular surface, and it was in the difficult situation to confirm the influence by the existence of a substrate. Therefore, we examined histopathologically the prognosis of cultivated epithelial sheets prepared from rabbitoral mucosa by using the rabbit model of limbus stem cell dysfunction. Cultivated epithelial sheets without substrate obtained good adhesion to corneal stroma and smooth ocular surface. On the other hand, cultivated epithelial sheets with substrate had low adhesion and epithelial cell invasion and vascular invasion accompanied with fibrosis from the peripheral underneath transplanted epithelial sheets were observed. Similar findings of vascular invasion underneath transplanted oral mucosal epithelial sheets also obtained in rabbit model. These data suggests the adhesiveness with transplanted oral mucosal epithelial sheets and corneal stroma may cause the vascular invasion underneath transplanted sheets. Less

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (3 results)

All 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (1 results)

  • [Journal Article] Long-term outcome of cultivated oral mucosal epithelial sheet transplantation in treatment of total limbal stem cell deficiency.2011

    • Author(s)
      Satake Y, Higa K, Tsubota K, Shimazaki J.
    • Journal Title

      Ophthalmology.

      Volume: 118 Issue: 8 Pages: 1524-30

    • DOI

      10.1016/j.ophtha.2011.01.039

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Long-term Outcome of Cultivated Oral Mucosal Epithelial Sheet Transpl antation in Treatment of Total Limbal Stem Cell Deficiency.2011

    • Author(s)
      Y.Satake, K.Higa, K.Tsubota, J.Shimazaki.
    • Journal Title

      Ophthalmology

      Volume: (In press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 角膜輪部機能不全に対する培養上皮シート移植後の二期的角膜移植の成績2010

    • Author(s)
      佐竹良之
    • Organizer
      第64回に本臨床眼眼科学会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-11-12
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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