Construction of functional glandular structures using tree-dimensional culture in mouse lacrimal gland
| Project/Area Number |
22591957
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | National Defense Medical College |
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Principal Investigator |
ITO Masataka 防衛医科大学校, 医学教育部医学科専門課程, 准教授 (30534896)
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| Co-Investigator(Kenkyū-buntansha) |
IMAKI Junko 防衛医科大学校, 医学教育部医学科専門課程, 教授 (20223323)
KARASAWA Yoko 防衛医科大学校, 医学教育部医学科専門課程, 助教 (60535540)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 3次元培養 / 再生医学 / 涙腺 / 発生分化 / ドライアイ / 細胞培養 / 外分泌腺 / 細胞生物学 / マウス / 幹細胞 / ドラアイアイ |
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| Research Abstract |
Mouse lacrimal gland epithelial cells were cultured in several three-dimensional conditions, but it was difficult to induce ductal structure. Then we cultured them in air-lift conditions. The epithelial cells differentiated to form epithelial tissues similar to cornea, conjunctiva and skin. We then analyzed the effects of EGF and HGF, these are paracrine factors secreted by lacrimal gland epithelial cells, in combination with their specific inhibitors and proved that EGF and HGF worked synergistically for cell proliferation and suppression of cell death in the development of lacrimal glands. These results of our experiments would provide clues for in vitro reorganization of functional glands from cultured cells for therapeutic purposes.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Accumulation of secretory vesicles in the lacrimal gland epithelia is related to non-Sjogren' s type dry eye in visual display terminal users2012
Author(s)
Kamoi M, Ogawa Y, Nakamura S, Dogru M, Nagai T, Obata H, Ito M, Kaido M, Kawakita T, Okada Y, Kawakami Y, Shimmura S, Tsubota K.
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Journal Title
PLoS One
Volume: 7巻
Issue: 9
Pages: 1-8
DOI
Related Report
Peer Reviewed
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