| Project/Area Number |
22591999
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YASUOKA Tomoyuki 岩手医科大学, 形成外科学講座, 助教 (10382607)
TONO Hisayuki 岩手医科大学, 形成外科学講座, 任期付助教 (10509003)
KURODA Kei 岩手医科大学, 形成外科学講座, 研究員 (30530224)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | マイクロサージェリー学 / 筋肉移植 / 神経再生 / アセチルコリン / レセプター / アセチルコリンレセプター / マイクロサージャリ- / 筋再生 / αブンガロトキシン / 蛍光染色 / マイクロサージャリー学 |
|---|
| Research Abstract |
Experimental study using the rectus femoris muscle of rabbits was performed to investigate the recovery process of the muscle transplantation with microneurovascular anastomosis.After 2~24 postoperative weeks, tissue specimens obtained from muscle tissue were examined for pathological analysis. In the pathological analysis, fluorescent staining was used for visualizing the muscle fibers and distribution of acetylcholine receptors together with H-E staining and Azan staining.Wet weight of transplanted muscle had gradually increased after it decreased to about half of control model in innervation model. Degeneration and recovery of muscle fibers were confirmed by H-E and Azan staining in the each model, although the comfortable result for statistical analysis could not be obtained in the fluorescent staining, because the determination of the distribution of acetylcholine receptor was unstable.
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