Functional roles of sphingosine-1-phosphate in mesenchymal stem cell migration and proliferation and bone metabolism
Project/Area Number |
22592000
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Plastic surgery
|
Research Institution | Saitama Medical University |
Principal Investigator |
SATO Tomoya 埼玉医科大学, 医学部, 助教 (10445132)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 間葉系幹細胞 / 骨代謝 / 骨芽細胞 / 再生医学 / スフィンゴシン1リン酸 / 細胞・組織 / 移植・再生医療 / 生体分子 / シグナル伝達 |
Research Abstract |
During bone formation, osteoblasts are derived from mesenchymal stem cells (MSCs) present in the bone marrow. Recent studies have indicated that sphingosine-1-phosphate (S1P) plays a vital role in various tissues; however, its role in bone metabolism remains unclear. Here, we demonstrated that S1P induces proliferation and migration of MSCs. We detected the mRNA expression of S1P receptor 1 (S1PR1) via reverse transcription polymerase chain reaction (RT-PCR) and determined whether S1P induces proliferation of MSCs. Treatment of MSCs with S1P for 24 h stimulated a dose-dependent increase in DNA synthesis. These data demonstrated that S1P is mitogenic to MSCs in vitro. S1P produced by osteoclast precursors during differentiation may function in a paracrine manner to MSCs. To explore this possibility, we examined the chemotactic effects of S1P on MSCs. S1P increased chemotaxis of MSCs; however, the differentiation of MSC to osteoblasts was not affected by S1P. These results suggest that S1P plays a possible role in regulating the proliferation and migration of MSCs.
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Report
(4 results)
Research Products
(52 results)