Mechanism of protective effects of atrial natriuretic peptide on acute kidney injury

• Project/Area Number: 22592010
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Emergency medicine
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: MITAKA Chieko 東京医科歯科大学, 医歯(薬)学総合研究科, 准教授 (20126254)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 急性腎傷害 / 心房性ナトリウム利尿ペプチド / サイトカイン / 炎症 / 虚血再灌流傷害 / 造影剤 / 腎虚血•再灌流 / 造影剤腎症 / 虚血・再灌流傷害 / 多臓器障害症候群 / 抗炎症反応 / 虚血・再灌流障害 / 多臓器不全症候群

Outline of Final Research Achievements

We investigated whether atrial natriuretic peptide (ANP) attenuates renal ischemia-reperfusion injury (IRI)-induced acute kidney injury (AKI) or contrast-induced AKI. ANP prevented renal IRI-induced metabolic acidosis, pulmonary edema, increased plasma concentrations of lactate, creatinine and potassium in rats. In addition, ANP attenuated renal IRI-induced increases in plasma concentrations of IL-1beta and IL-6 as well as elevation of TNF-alpha, IL-1beta, IL-6 mRNA and TNF-alpha, IL-6, NF-kappaB protein expressions in kidney and lung. These findings suggest that ANP prevents kidney-lung cross talk by inhibiting inflammatory cytokines in rat renal IRI model. Furthermore, pretreatment of ANP attenuated contrast-induced AKI in rats.

Research Products

• [Journal Article] Atrial natriuretic peptide attenuates kidney-lung crosstalk in kidney injury (2014)
  • Author(s): Tulafu M, Mitaka C, Hnin Si MK, Abe S, Kitagawa M, Ikeda S, Eishi Y, Kurata S, Tomita M
  • Journal Title: J Surg Res Volume: 186 Issue: 1 Pages: 217-225
  • DOI: 10.1016/j.jss.2013.07.033
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Effects of atrial natriuretic peptide on inter-organ crosstalk among the kidney, lung, and heart in a rat model of renal ischemia-reperfusion injury (2014)
  • Author(s): Mitaka C, Hnin Si MK, Tulafu M, Qi Y, Uchida T, Abe S, Kitagawa M, Ikeda S, Eishi Y, Tomita M
  • Journal Title: Intensive Care Medicine Experimental Volume: 2 Issue: 1 Pages: 28-44
  • DOI: 10.1186/s40635-014-0028-8
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Atrial natriuretic peptide attenuates metabolic acidosis and inflammation of kidney, lung and heart in a rat model of renalischemia-reperfusion injury (2013)
  • Author(s): Miniwan Tulafu
  • Organizer: 第40回日本集中治療医学会学術集会
  • Place of Presentation: 松本市、長野県
• [Presentation] Atrial natriuretic peptide attenuates metabolic acidosis and inflammation of kidney, lung and heart in a rat model of renalischemia-reperfusion injury (2013)
  • Author(s): May Khin Hnin Si
  • Organizer: 33rd International Symposium on Intensive Care and Emergency Medicine
  • Place of Presentation: Brussels, Belgium
• [Presentation] Atrial natriuretic peptide prevents acute kidney injury in renal ischemia-reperfusion injury rat model (2012)
  • Author(s): Miniwan Tulafu
  • Organizer: 第39回日本集中治療医学会学術集会
  • Place of Presentation: 千葉、幕張メッセ
  • Year and Date: 2012-02-29
• [Presentation] Atrial natriuretic peptide prevents acute kidney injury in renal ischemia-reperfusion injury rat model (2012)
  • Author(s): Miniwan Tulafu
  • Organizer: Critical Care Congress
  • Place of Presentation: Houston, Texas, USA
  • Year and Date: 2012-02-05
• [Presentation] Atrial natriuretic peptide attenuates contrast-induced nephropathy in rats
  • Author(s): Chieko Mitaka
  • Organizer: 第41回日本集中治療医学会学術集会
  • Place of Presentation: 京都