Verification of paradigm for differentiation, interrelation, tissue organization of epithelial and mesenchymal cells in root formation

• Project/Area Number: 22592028
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Hokkaido University
• Principal Investigator: YAMAMOTO Tsuneyuki 北海道大学, 大学院・歯学研究科, 准教授 (80200822)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ヘルトビッヒ上皮鞘 / セメント芽細胞 / セメント質

Research Abstract

This study was designed to investigate the mechanism of disintegration of Hertwig’s epithelial sheath and the behavior of the epithelial sheath cells and dental follicle cells. Developing rat molars were examined by immunohistochemistry using antibodies to several kinds of matrix-degrading enzymes and alkaline phosphatase. Findings suggest that: (1) The epithelial sheath cells secrete the enzymes to break intercellular attachment devices and the sheath disintegrates. (2)After the sheath disintegration the epithelial cells do not transform into cementoblasts and surviveas epithelial rests of Malassez. (3)Cementoblasts derive from the dental follicle.

Research Products

• [Journal Article] Histochemical examination of vascular medial calcification of aorta in klotho-deficient mice. (2013)
  • Author(s): Hasegawa T., Amizuka N., et al
  • Journal Title: Journal of Oral Biosciences Volume: 55 Issue: 1 Pages: 10-15
  • DOI: 10.1016/j.job.2012.12.003
  • Peer Reviewed
• [Journal Article] Morphological aspects of the biological function of osteocytic lacunar canalicular system and osteocyte-derived factors (2012)
  • Author(s): Sasaki M, Hongo H, Hasegawa T, Suzuki R, Liu Z, Freitas Paulo HL, Yamada T, Oda K, Yamamoto T , Li M, Totsuka y, Amizuka N
  • Journal Title: Oral Science International Volume: 9 Issue: 1 Pages: 1-8
  • DOI: 10.1016/s1348-8643(12)00009-2
  • NAID: 10031144841
  • Peer Reviewed
• [Journal Article] Immunolocalization of sclerostin synthesized by osteocytes in relation to bone remodeling in the interradicular septa of ovariectomized rats. (2012)
  • Author(s): Guo Y., Hasegawa T., Amizuka N., et al
  • Journal Title: Journal of Electron Microscopy Volume: 61(5) Issue: 5 Pages: 309-320
  • DOI: 10.1093/jmicro/dfs052
  • Peer Reviewed
• [Journal Article] Structure and formation of the twisted plywood pattern of collagen fibrils in rat lamellar bone (2012)
  • Author(s): Yamamoto T., Hasegawa T., Sasaki M., Hongo H., Tabata C., Liu Z., Li M., Amizuka N
  • Journal Title: J. Electron Microsc Volume: 61 Issue: 2 Pages: 113-121
  • DOI: 10.1093/jmicro/dfs033
  • Peer Reviewed
• [Journal Article] Morphological assessment of bone mineralization in tibial metaphysic of ascorbic acid-deficient ODS rats (2011)
  • Author(s): Hasegawa T, Li M, Hara K, Sasaki M, Tabata C, de Freitas Paulo HL, Hongo H, Suzuki R, Kobayashi M, Inoue K, Yamamoto T ,Oohata N, Oda K, Akiyama Y, Amizuka N
  • Journal Title: Biomedical Res Volume: 32 Pages: 259-269
  • NAID: 130004903823
  • URL: https://www.jstage.jst.go.jp/browse/biomedres
  • Peer Reviewed
• [Journal Article] Ultrastructural observation on cells meeting the histological criteria for preosteoblasts a study in the mouse tibial methaphysis (2010)
  • Author(s): Narimatsu K, Li M, Freitas Paulo HL, Sultana S, Ubaidus S, Kojima T, Liu Z, Guo Y, Suzuki R, Yamamoto T , Oda k, Amizuka N
  • Journal Title: J Electron Microsc Volume: 59 Issue: 5 Pages: 427-436
  • DOI: 10.1093/jmicro/dfq021
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of periodontal reattachment on denuded root dentin after periodontal surgery (2010)
  • Author(s): Harahashi H, Odajima T, Yamamoto T , Kawanami M
  • Journal Title: Biomedical research Volume: 31 Pages: 319-328
  • NAID: 130004903811
  • URL: https://www.jstage.jst.go.jp/browse/biomedres
  • Peer Reviewed
• [Journal Article] Immunolocalization of DMP1 and sclerostin in the epiphyseal trabecule and diaphyseal cortical bone of osteoprotegerin deficient mice (2010)
  • Author(s): Masuki H, Li M, Hasegawa T, Suzuki R, Guo Y, Liu Z, Oda K, Yamamoto T , Kawanami M, Amizuka N
  • Journal Title: Biomedical Res Volume: 31 Pages: 307-318
  • NAID: 130004903810
  • URL: https://www.jstage.jst.go.jp/browse/biomedres
  • Peer Reviewed
• [Journal Article] Histological review of the human cellular cementum with special reference to an alternating lamellar pattern (2010)
  • Author(s): Yamamoto T , Li M, Liu Z, Guo Y, Hasegawa T, Masuki H, Suzuki R, Amizuka N
  • Journal Title: Odontology Volume: 98 Pages: 102-109
  • NAID: 10029302597
  • Peer Reviewed
• [Presentation] 長骨の部位による骨細胞ネットワークの立体微細形態の違いについて (2012)
  • Author(s): 山本恒之,長谷川智香,佐々木宗輝,因幡千尋,本郷裕美,山田珠希,網塚憲生
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 京王プラザホテル東京
• [Presentation] 副甲状腺ホルモン投与で誘導される骨小腔周囲の骨基質溶解について (2012)
  • Author(s): 本郷裕美,佐々木宗輝,長谷川智香,山田珠希,因幡千尋,山本恒之,中野貴由,網塚憲生
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 京王プラザホテル東京
• [Presentation] 長骨の部位による骨細胞ネットワークの立体微細形態の違いについて (2012)
  • Author(s): 山本恒之
  • Organizer: 骨代謝学会
  • Place of Presentation: 京王プラザホテル（東京都）
• [Presentation] アルカリフォスファターゼトランスジェニックマウスにおける基質石灰化機構の解明 (2011)
  • Author(s): 長谷川智香、織田公光、佐々木宗輝、田幡千尋、柳鋳晟、郭穎、井上貴一朗、李敏啓、小守壽文、山本恒之、網塚憲生
  • Organizer: 第29回日本骨代謝学会学
  • Place of Presentation: 大阪国際会議場大阪
• [Presentation] Klotho-/-環境における骨細胞は基質ミネラル維持不能を示す (2011)
  • Author(s): 佐々木宗輝、田幡千尋、長谷川智香、郭穎、柳鋳晟、李敏啓、山本恒之、井上農夫男、網塚憲生
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場大阪
• [Presentation] 血管石灰化における微細構造学的機序の解明-Klotho-/-マウス血管平滑筋細胞のtrans-differentiationと基質小胞形成 (2011)
  • Author(s): 長谷川智香、李敏啓、佐々木宗輝、田幡千尋、大城戸一郎、郭穎、柳鋳晟、山本恒之、横山啓太郎、網塚憲生
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪国際会議場大阪