Effect of oral bacteria on intestinal innate immunity

• Project/Area Number: 22592029
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Hokkaido University
• Principal Investigator: NAKAMURA Kiminori 北海道大学, 大学院・先端生命科学研究院, 助教 (80381276)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 自然免疫 / 細胞・組織

Research Abstract

To analyze the interaction of oral bacteria and intestinal epithelia cells, the aim of this study is to establish a intestinal epithelial cell line containing Paneth cells which secrete α -defensins. In conventional monolayer cell culture, intestinal epithelial cells were observed, however, Paneth cells were not identified. Next, organoid culture were performed by using mouse intestinal crypts. It was analyzed whetherobtained organoids were maintaining the function of a Paneth cell.

Research Products

• [Journal Article] Intracellular superoxide dismutase activity defines invasiveness of the murine T-lymphoma cell line L5187Y-ML25 in vitro and in vivo (2013)
  • Author(s): Tanaka M, Kuribayashi K, Kogawa K, NakamuraK , Watanabe N
  • Journal Title: Leuk Res Volume: 37(1) Pages: 89-92
  • Peer Reviewed
• [Journal Article] Intracellular superoxide dismutase activity defines invasiveness of the murine T-lymphoma cell line L5187Y-ML25 in vitro and in vivo. (2013)
  • Author(s): Tanaka M
  • Journal Title: Leuk Res Volume: 37 Issue: 1 Pages: 89-92
  • DOI: 10.1016/j.leukres.2012.07.021
  • Peer Reviewed
• [Journal Article] Graft-versus-host disease disrupts intestinal microbial ecology by inhibiting Paneth cell production of α-defensins (2012)
  • Author(s): Eriguchi Y, Takashima S, Oka H, Shimoji S, Nakamura K , Uryu H, Shimoda S, Iwasaki H, Shimono N, Ayabe T, Akashi K, Teshima T
  • Journal Title: Blood Volume: 120 (1) Pages: 223-31
  • Peer Reviewed
• [Journal Article] Paneth cells and stem cells in the intestinal stem cell niche and their association with inflammatory bowel disease (2012)
  • Author(s): NakamuraK , Ayabe T
  • Journal Title: Inflammation Regeneration. Volume: 32 Pages: 53-60
  • NAID: 130004482294
  • Peer Reviewed
• [Journal Article] 腸内環境を制御するPaneth細胞αディフェンシン (2012)
  • Author(s): 綾部時芳、中村公則
  • Journal Title: 臨床免疫・アレルギー科 Volume: 57(1) Pages: 25-31
• [Journal Article] Graft-versus-host disease disrup ts intestinal microbial ecology by i nhibiting Paneth cell production of α-defensins (2012)
  • Author(s): Eriguchi Y, Takashima S 他10名
  • Journal Title: Blood Volume: 120巻 Issue: 1 Pages: 223-231
  • DOI: 10.1182/blood-2011-12-401166
  • Peer Reviewed
• [Journal Article] Paneth cells and stem cells in the intestinal stem-cell niche and their association with inflamatory bowel disease (2012)
  • Author(s): Nakamura K, Ayabe T
  • Journal Title: Inflammation Regeneration Volume: 32 Pages: 53-60
  • Peer Reviewed
• [Journal Article] Regulation of microbiota by antimicrobial peptides in the gut (2011)
  • Author(s): Masuda K, NakamuraK , Yoshioka S, Fukaya R, Sakai N, Ayabe T
  • Journal Title: Adv Otorhinolaryngol Volume: 72 Pages: 97-9
• [Journal Article] EpCAM- and EGFR-targeted selective gene therapy for biliary cancers using Z33-fiber-modified adenovirus (2011)
  • Author(s): Kawashima R, Abei M, Fukuda K, NakamuraK , Murata T, Wakayama M, Seo E, Hasegawa N, Mizuguchi H, Obata Y, Hyodo I, Hamada H, Yokoyama KK
  • Journal Title: Int J Cancer Volume: 129 (5) Pages: 1244-53
  • Peer Reviewed
• [Journal Article] Neural cell adhesion molecule 2 as a target molecule for prostate and breast cancer gene therapy (2011)
  • Author(s): Takahashi S, Kato K, NakamuraK , Nakano R, Kubota K, Hamada H
  • Journal Title: Cancer Sci Volume: 102(4) Pages: 808-14
  • NAID: 10029292451
  • Peer Reviewed
• [Journal Article] Bactericidal activity of mouse α-defensin cryptdin-4 predominantly affects noncommensal bacteria (2011)
  • Author(s): Masuda K, Sakai N, NakamuraK , Yoshioka S, Ayabe T
  • Journal Title: J Innate Immun Volume: 3(3) Pages: 315-26
  • NAID: 120002989233
  • Peer Reviewed
• [Journal Article] αディフェンシンによる腸内細菌制御 (2011)
  • Author(s): 中村公則、綾部時芳
  • Journal Title: 実験医学 Volume: 29(18) Pages: 2955-2961
• [Journal Article] Regulation of microbiota by antimicrobial peptides in the gut (2011)
  • Author(s): Masuda K, Nakamura K, Yoshioka S, Fukaya R, Sakai N, Ayabe T
  • Journal Title: Adv Otorhinolaryngol Volume: 72 Pages: 97-99
  • DOI: 10.1159/000324625
  • ISBN: 9783805597227, 9783805597234
  • Peer Reviewed
• [Journal Article] Bactericidal activity of mouse α-defensin cryptdin-4 predominantly affects noncommensal bacteria (2011)
  • Author(s): Masuda K, Sakai N, Nakamura K, Yoshioka S, Ayabe T
  • Journal Title: J Innate Immun Volume: 3 Issue: 3 Pages: 315-326
  • DOI: 10.1159/000322037
  • NAID: 120002989233
  • Peer Reviewed
• [Presentation] Deficiency of secreted cryptdin-4 detected in a mouse model of Crohn ' s disease using a new sandwich ELISA (2012)
  • Author(s): Nakamura K
  • Organizer: Experimental Biology 2012
  • Place of Presentation: San Diego Convention Center (USA)
  • Year and Date: 2012-04-25
• [Presentation] Deficiency of secreted cryptdin-4 detected in a mouse model of Crohn’s disease using a new sandwich ELISA.
  • Author(s): Nakamura K
  • Organizer: Experimental Biology 2012
  • Place of Presentation: San Diego Convention Center (USA)