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Maintenance mechanism of oral homeostasis revealed by fine scale analysis of microbiota

Research Project

Project/Area Number 22592032
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

MARUYAMA Fumito  東京医科歯科大学, 大学院・医歯学総合研究科, 准教授 (30423122)

Co-Investigator(Kenkyū-buntansha) SAKURAI Atsuo  東京歯科大学, 小児歯科講座, 助教 (90431759)
NAKAGAWA Ichiro  東京医科歯科大学, 大学院・医歯学総合研究科, 教授 (70294113)
KUROKAWA Ken  東京工業大学, 大学院・生命理工学研究科, 教授 (20343246)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords細菌叢 / 新型シーケンサー / 恒常性 / 口腔細菌叢 / 細菌ゲノム / 高速シーケンサー
Research Abstract

The oral cavity is exposed to many complex substances and is the first door for bacteria to invade the body. The goal of this study is to perform the oral flora analysis precisely and is intended to clarify the mechanism that how constancy of the oral microbial environment is maintained. The oral environment changes complicatedly, and there is no data about Archaea. Therefore comparative bacterial community analysis was carried out using normal oral flora, caries and periodontal disease, aimed at the elucidation of the oral constancy maintenance mechanism through isolation and genome determination of the key bacteria to the homeostasis. As a result, in the children of around 10 years old, the bacteria were not different with those of an adult. On the other hand, as a result of the genome analysis of the isolated strains, bacterial survival in the niche could be affected greatly by CRISPR which was a bacterial acquired immunity mechanism. The knowledge becomes the base of future metagenome analysis.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (46 results)

All 2013 2012 2011 2010 Other

All Journal Article (26 results) (of which Peer Reviewed: 23 results) Presentation (9 results) Book (5 results) Remarks (6 results)

  • [Journal Article] Diversity and Universality of Capsular Polysaccharide Synthesis Gene Clusters in Streptococcus suis2013

    • Author(s)
      D. Takamatsu and F. Maruyama
    • Journal Title

      Appl. Environ. Microbiol

      Volume: 79 Pages: 2493-2493

    • Related Report
      2012 Final Research Report
  • [Journal Article] GeneticAnalysis of Capsular Polysaccharide Synthesis Gene Clusters from All Serotypes of Streptococcus suis:Potential Mechanisms for theGeneration of Capsular Variation2013

    • Author(s)
      M. Okura, D. Takamatsu, F.Maruyama, T. Nozawa, I. Nakagawa, M. Osaki, T. Sekizaki, M. Gottschalk,Y. Kumagai, and S. Hamada
    • Journal Title

      Appl. Environ. Microbiol

      Volume: 79 Pages: 2796-2806

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Complete genome sequence of Propionibacterium acnes isolate from sarcoidosis patient2013

    • Author(s)
      K. Minegishi, C. Aikawa, A.Furukawa, T. Watanabe, T. Nakano, Y. Ogura, Y. Ohtsubo, K. Kurokawa, T. Hayashi, F. Maruyama, I. Nakagawa,Y. Eishi
    • Journal Title

      Genome Announc

      Volume: 1

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Complete Genome Sequence of Acidovorax sp.KKS102, a Polychlorinated Biphenyl-Degrading strain2012

    • Author(s)
      Y. Ohtsubo, F. Maruyama, H. Mitsui, Y. Nagata, M. Tsuda
    • Journal Title

      J. Bacteriol

      Volume: 194 Pages: 6970-1

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phytate addition to soil induces changes in the abundance and expression of Bacillus β-propeller phytase genes in the rhizosphere2012

    • Author(s)
      M. Jorquera, N. Saavedra, F.Maruyama, A. Richardson, D. Crowley, R. Catrilaf, E. Henriquez, M. Mora
    • Journal Title

      FEMS Microbiol. Ecol

      Volume: 83 Pages: 352-60

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The small GTPases Rab9A and Rab23 function at distinct steps in autophagyduring Group A Streptococcus infection.2012

    • Author(s)
      -T. Nozawa, C. Aikawa, A. Goda, F.Maruyama, S. Hamada, I. Nakagawa
    • Journal Title

      Cell. Microbiol

      Volume: 14 Pages: 1149-1165

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Complete Genome Sequence of the serotype k Streptococcus mutans LJ232012

    • Author(s)
      C. Aikawa, N. Furukawa, T. Watanabe, K. Minegishi, A. Furukawa, Y. Eishi,K. Oshima, K. Kurokawa, M. Hattori,K. Nakano, *F. Maruyama, I. Nakagawa and T. Ooshima
    • Journal Title

      J. Bacteriol

      Volume: 194 Pages: 2754-2754

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Novel conjugative transferable multiple drug resistance plasmid pAQU1 from Photobacterium damselae subsp. damselae isolated from marine aquaculture environment2012

    • Author(s)
      L. Nonaka, F. Maruyama, M.Miyamoto, M. Miyakoshi, K. Kurokawa, M. Masuda
    • Journal Title

      Microb.Environ

      Volume: 27 Pages: 263-272

    • NAID

      10030869338

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transition metal ions induce carnosinase activity in PepD-homologous protein from Porphyromonas gingivalis TDC60 with type II fimA2012

    • Author(s)
      A. Aoki, Y. Shibata, S. Okano, F.Maruyama, A. Amano, I. Nakagawa,*Y. Abiko
    • Journal Title

      Microb. Pathog

      Volume: 52 Pages: 17-24

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Complete genome sequence of the serotype k Strept ococcus mutans strain LJ23.2012

    • Author(s)
      Aikawa C, Furukawa N, Watanabe T, Minegishi K, Furukawa A, Eishi Y, Oshima K, Kurukawa K, Hattori M, Nakano K, Maruyama F, Nakagawa I, Ooshima T
    • Journal Title

      Journal of Bacteriology

      Volume: 194 Issue: 10 Pages: 2754-2755

    • DOI

      10.1128/jb.00350-12

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transition metal ions induce carnosinase activity in PepD-homologous protein from Porphyromonas gingivalis.2012

    • Author(s)
      Aoki A, Shibata Y, Okano S, Maruyama F, Amano A, Nakagawa I, Abiko Y
    • Journal Title

      Microb Pathog.

      Volume: Vol52 Issue: 1 Pages: 17-24

    • DOI

      10.1016/j.micpath.2011.09.003

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomic structure of the cyanobacterium Synechocystis sp. PCC 6803 strain GT-S2011

    • Author(s)
      N. Tajima, S. Sato, F. Maruyama, N.Kaneko, V. Sasaki, K. Kurokawa, H.Ohta, Y. Kanesaki, H. Yoshikawa, S. Tabata, M. Ikeuchi and *N. Sato
    • Journal Title

      DNA Res

      Volume: 18 Pages: 393-399

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Complete Genome Sequence of the Periodontogenic Bacterium Porphyromonas gingivalis TDC602011

    • Author(s)
      F. Maruyama, T. Watanabe, T. Nozawa, A. Aoki, S. Okano, Y. Shibata, K. Oshima, K. Kurokawa, M. Hattori, I. Nakagawa, Y. Abiko
    • Journal Title

      J. Bacteriol

      Volume: 193 Pages: 4259-4260

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] CRISPR inhibition of prophage acquisition in Streptococcus pyogenes2011

    • Author(s)
      T. Nozawa, N. Furukawa, C. Aikawa, T. Watanabe, B. Haobam, K. Kurokawa, *F. Maruyama, I. Nakagawa
    • Journal Title

      PLoS One

      Volume: 6

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] CRISPR inhibition of prophage acquisition in Streptococcus pyogenes2011

    • Author(s)
      T.Nozawa
    • Journal Title

      PLoS One

      Volume: 6 Issue: 5 Pages: e19543-e19543

    • DOI

      10.1371/journal.pone.0019543

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Complete genome sequence of the bacterium Porphyromonas gingivalis TDC60, which causes periodontal disease2011

    • Author(s)
      Watanabe T, Maruyama F, Nozawa T, Aoki A, Okano S, Shibata Y, Oshima K, Kurokawa K, Hattori M, Nakagawa I, Abiko Y
    • Journal Title

      J Bacteriol

      Volume: 193 Issue: 16 Pages: 4259-4260

    • DOI

      10.1128/jb.05269-11

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomic structure of the cyanobacterium Synechocystis sp.PCC 6803 strain GT-S2011

    • Author(s)
      Tajima Naoyuki
    • Journal Title

      DNA Research

      Volume: 18 Issue: 5 Pages: 393-399

    • DOI

      10.1093/dnares/dsr026

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The dawning era of comprehensive transcriptome analysis in cellular microbiology2010

    • Author(s)
      C. Aikawa, F. Maruyama, I. Nakagawa
    • Journal Title

      Front. Microbiol

      Volume: 1 Pages: 118-118

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] visualization tool for taxonomic compositions of microbial communities based on 16S rRNA gene sequences2010

    • Author(s)
      H. Mori, F. Maruyama, K. Kurokawa.VITCOMIC
    • Journal Title

      BMC Biofinfo

      Volume: 11 Pages: 332-332

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Commensal Proteobacteria in colonic CD11b+ cellsinduce intestinal T cell responses2010

    • Author(s)
      -I. Takahashi, F. Maruyama, Y. Kurashima, Y. Goto, T. Obata, I. Nakagawa, *H. Kiyono
    • Journal Title

      Int. Immunol

      Volume: 22

    • Related Report
      2012 Final Research Report
  • [Journal Article] The specific behavior of intracellular Streptococcus pyogenes undergone the autophagic degradation is associated withbacterial streptolysin O and host small G proteins Rab5 and Rab72010

    • Author(s)
      A. Sakurai, F. Maruyama, J. Funao, T.Nozawa, C. Aikawa, N. Okahashi, S. Shintani, S. Hamada, *T. Ooshima, I.Nakagawa
    • Journal Title

      J. Biol. Chem

      Volume: 285 Pages: 22666-22675

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The dawning era of comprehensive transcriptome analysis in cellular microbiology.2010

    • Author(s)
      Aikawa Chihiro
    • Journal Title

      Frontiers in Microbiology

      Volume: 1 Pages: 118-118

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] VITCOMIC:visualization tool for taxonomic compositions of microbial communities based on 16S rRNA gene sequences.2010

    • Author(s)
      Mori Hiroshi
    • Journal Title

      BMC bioinformatics

      Volume: 11 Pages: 332-332

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Commensal Proteobacteria in colonic CD 11 b^<+> cells induce intestinal T cell responses.2010

    • Author(s)
      Takahashi Ichiro
    • Journal Title

      International Immunology

      Volume: 22

    • Related Report
      2010 Annual Research Report
  • [Journal Article] The specific behavior of intracellular Streptococcus pyogenes undergone the autophagic degradation is associated with bacterial streptolysin O and host small G proteins Rab5 and Rab7.2010

    • Author(s)
      Sakurai Atsuo
    • Journal Title

      Journal of Biological Chemistry

      Volume: 285 Pages: 22666-22675

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reactive oxygen species induced by fibronectin-mediated Streptococcus pyogenes invasion trigger apoptotic cell death in infected epithelial cells.2010

    • Author(s)
      Aikawa Chihiro
    • Journal Title

      Cellular Microbiology

      Volume: 12 Pages: 814-830

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Species-specific strategy of genome diversification in genus streptococcivia prophage acquisition and its defense system2011

    • Author(s)
      Maruyama Fumito
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      2011-09-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] NLRX1 promotes the GAS induced-autophagy in epithelial cells.2010

    • Author(s)
      Aikawa Chihiro
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] Rabタンパク質によるA群レンサ球菌感染誘導オートファジーの制御機構2010

    • Author(s)
      野澤孝志
    • Organizer
      第52回歯科基礎医学会学術大会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] A群レンサ球菌感染誘導オートファジーにおけるユビキチンシステムの機能解析2010

    • Author(s)
      野澤孝志
    • Organizer
      第4回細菌学・若手コロッセウム
    • Place of Presentation
      ラフォーレ修善寺(静岡県)
    • Year and Date
      2010-08-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Fluctuation of gene pools of soil microbiota induced by contamination with aromatic hydrocarbons.2010

    • Author(s)
      Kato Hiromi
    • Organizer
      13th International Society for Microbial Ecology Conference
    • Place of Presentation
      Washington State Convention and Trade Center(USA)
    • Year and Date
      2010-08-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Commensal Pmteobacteria in colonic CD 11b^<+> cells induce intestinal T cell responses.2010

    • Author(s)
      Takahashi Ichiro
    • Organizer
      14th International Congress of Immunology
    • Place of Presentation
      Kobe Convention CenterComplex(Japan)
    • Year and Date
      2010-08-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細菌ゲノムにおける水平伝播したオペロンの検出2010

    • Author(s)
      野口恵
    • Organizer
      進化学会第12回東京大会
    • Place of Presentation
      東京工業大学大岡山キャンパス(東京都)
    • Year and Date
      2010-08-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] 第二世代シークエンサを用いた、土壌汚染に伴う微生物の適応反応の解析2010

    • Author(s)
      加藤広海
    • Organizer
      環境バイオテクノロジー学会2010年度大会
    • Place of Presentation
      東北大学片平さくらホール(宮城県)
    • Year and Date
      2010-06-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] A small GTPase, Rab23, is required for the autophagic degradation of intracellular group A streptococcus.2010

    • Author(s)
      Nozawa Takashi
    • Organizer
      110th ASM General Meeting
    • Place of Presentation
      San Diego Convention Center(USA)
    • Year and Date
      2010-05-23
    • Related Report
      2010 Annual Research Report
  • [Book] 化学と生物2013

    • Author(s)
      渡辺孝康、中川一路、丸山史人
    • Publisher
      原核生物の新規な獲得免疫機構CRISPR/Cas システム
    • Related Report
      2012 Final Research Report
  • [Book] 第2章メタゲノムデータベース. メタゲノム解析技術の最前線. Metagenomics:a new frontier of bacterial community researches2010

    • Author(s)
      森宙史、丸山史人、*黒川顕
    • Publisher
      CMC 出版,東京
    • Related Report
      2012 Final Research Report
  • [Book] 第8章メタゲノムインフォマティクス.難培養微生物研究の最新技術 II - ゲノ ム解析を中心とした最前線と将来展望- Current Technology and Perspectives for Yet-uncultivated Microbial Resources2010

    • Author(s)
      森宙史、丸山史人、黒川顕
    • Publisher
      CMC 出版,東京
    • Related Report
      2012 Final Research Report
  • [Book] メタゲノム解析技術の最前線2010

    • Author(s)
      森宙史
    • Total Pages
      12
    • Publisher
      CMC出版
    • Related Report
      2010 Annual Research Report
  • [Book] 難培養微生物研究の最新技術II2010

    • Author(s)
      森宙史
    • Total Pages
      10
    • Publisher
      CMC出版
    • Related Report
      2010 Annual Research Report
  • [Remarks] 東京医科歯科大学・大学院医歯学総合研究科・細菌感染制御学分野

    • URL

      http://www.tmd.ac.jp/grad/bac/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 東京医科歯科大学・大学院医歯学総合研究科・環境遺伝生態学分野

    • URL

      https://www.facebook.com/pages/Microbial-Genomics-and-Ecology/135689926563239

    • Related Report
      2012 Final Research Report
  • [Remarks] 東京医科歯科大学大学院医歯学総合研究科細菌感染制御学分野ホームページ

    • URL

      http://www.tmd.ac.jp/grad/bac/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks] 東京医科歯科大学大学院医歯学総合研究科環境遺伝性退学分野ホームページ

    • URL

      https://www.facebook.com/pages/Microbial-Genomics-and-Ecology/135689926563239

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/grad/bac/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.tmd.ac.jp/grad/bac/

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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