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Molecular mechanisms of ghost cell formation and calcification in calcifying cystic odontogenic tumor (CCOT) cell lines

Research Project

Project/Area Number 22592033
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Morphological basic dentistry
Research InstitutionNiigata University

Principal Investigator

CHENG Jun  新潟大学, 医歯学系, 准教授 (40207460)

Co-Investigator(Kenkyū-buntansha) MARUYAMA Satoshi  新潟大学, 医歯学総合病院, 講 師 (30397161)
YAMAZAKI Manabu  新潟大学, 医歯学系, 助 教 (10547516)
SAKU Takashi  新潟大学, 医歯学系, 教 授 (40145264)
ABE Tatsuya  新潟大学, 医歯学総合病院, レジデント (70634856)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords歯原性腫瘍 / 石灰化歯原性嚢胞 / 幻影細胞 / 石灰化 / 細胞外基質 / 細胞培養 / 脱核 / 免疫組織化学 / RT-PCR
Research Abstract

Calcifying cystic odontogenic tumor (CCOT) is histopathologically characterized by the emergence of ghost cells. However, their histopathogenesis has been poorly understood. To understand the cellular mechanism towards ghost cell formation, we have successfully established of cell lines from a CCOT surgical specimen. Using these CCOT cells, we successfully demonstrated that ghost cells were generated due to intracellular deposits of extracellular matrix molecules including perlecan, and that their calcification was started in those matrices and completed by proteolysis of deposited matrices towards their denucleated forms.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (22 results)

All 2014 2012 2011 2010 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (12 results) Remarks (3 results)

  • [Journal Article] Intramuscular keratocyst as a soft tissue counterpart of keratocystic odontogenic tumor : differential diagnosis by immunohistochemistry2014

    • Author(s)
      Abe T, Maruyama S, Yamazaki M, Essa A, Babkair H, Mikami T, Shingaki S, Kobayashi T, Hayashi T, Cheng J, Saku T
    • Journal Title

      Human Pathology

      Volume: 45(1) Pages: 110-118

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ghost cell odontogenic carcinoma arising in the background of a benign calcifying cystic odontogenic tumor of the mandible2012

    • Author(s)
      Arashiyama T, Kodama Y, Kobayashi T, Hoshina H, Takagi R, Hayashi T, Cheng J, Saku T
    • Journal Title

      Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology

      Volume: 114(3) Issue: 3 Pages: e35-e40

    • DOI

      10.1016/j.oooo.2012.01.018

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Parenchymal-stromal switching for extracellular matrix production on invasion of oral squamous cell carcinoma2012

    • Author(s)
      Metwaly H, Maruyama S, Yamazaki M, Tsuneki M, Abe T, Jen KY, Cheng J, Saku T
    • Journal Title

      Human Pathology

      Volume: 43 (11) Pages: 1973-1981

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Podoplanin expression profiles characteristic of odontogenic tumor-specific tissue architectures2012

    • Author(s)
      Masayuki Tsuneki
    • Journal Title

      Pathology-Research and Practice

      Volume: 208 Issue: 3 Pages: 140-146

    • DOI

      10.1016/j.prp.2011.12.016

    • Related Report
      2012 Annual Research Report 2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Parenchymal-stromal switching for extracellular matrix production on invasion of oral squamous cell carcinoma2012

    • Author(s)
      Metwaly H, Maruyama S, Yamazaki M, Tsuneki M, Abe T, Jen KY, Cheng J, Saku T.
    • Journal Title

      Human Pathology

      Volume: 43(11) Issue: 11 Pages: 1973-1981

    • DOI

      10.1016/j.humpath.2012.02.006

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions : its practical in surgical pathology2010

    • Author(s)
      Tsuneki M, Yamazaki M, Cheng J, Maruyama S, Kobayashi T, Saku T
    • Journal Title

      Histopathology

      Volume: 57(6) Issue: 6 Pages: 806-813

    • DOI

      10.1111/j.1365-2559.2010.03721.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions : itspractical use in surgical pathology2010

    • Author(s)
      Tsuneki M
    • Journal Title

      Histopathology

      Volume: 576 Pages: 806-813

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 側頭筋-咬筋内嚢胞性病変2012

    • Author(s)
      阿部達也,丸山 智,山崎 学,アーメッド エッサ,新垣 晋,齊藤 力,林 孝文,程 〓,朔 敬
    • Organizer
      第23回日本臨床口腔病理学会総会・学術大会
    • Place of Presentation
      東京医科歯科大学M&D タワー鈴木章夫記念講堂 (東京都文京区)
    • Related Report
      2012 Final Research Report
  • [Presentation] A clinicopathological analysis of intraductal papilloma of minor salivary gland origin2012

    • Author(s)
      Cheng J, Wu L, Maruyama S, Yamazaki M, Saku T
    • Organizer
      Japan-China Dental Conference 2012
    • Place of Presentation
      West China College of Stomatology, Sichuan University (Chengdu, China)
    • Related Report
      2012 Final Research Report
  • [Presentation] A clinicopathological analysis of intraductal papilloma of minor salivary gland origin2012

    • Author(s)
      Cheng J
    • Organizer
      Japan-China dental conference 2012
    • Place of Presentation
      Chengdu, China
    • Related Report
      2012 Annual Research Report
  • [Presentation] 側頭筋-頬筋内嚢胞性病変2012

    • Author(s)
      阿部 達也
    • Organizer
      第23回日本臨床口腔病理学会総会・学術大会
    • Place of Presentation
      東京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] Intraductal papilloma with peri-luminal extension of the minor salivary gland2011

    • Author(s)
      Cheng J, Wu L, Maruyama S, Yamazaki M, Saku T
    • Organizer
      第22回日本臨床口腔病理学会総会・学術大会・第5回アジア口腔病理学会
    • Place of Presentation
      九州大学医学部百年講堂 (福岡市東区)
    • Related Report
      2012 Final Research Report
  • [Presentation] Intraductal papilloma with peri-luminal extension of the minor salivary gland2011

    • Author(s)
      Cheng J
    • Organizer
      第22回日本臨床口腔病理学会・第5回アジア口腔病理学会
    • Place of Presentation
      福岡市・九州大学医学部百年講堂
    • Related Report
      2011 Annual Research Report
  • [Presentation] Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions: its practical in surgical pathology2010

    • Author(s)
      Tsuneki M, Yamazaki M, Cheng J, Maruyama S, Saku T
    • Organizer
      15^<th> International Congress on Oral Pathology and Medicine. International Association of Oral Pathologists 2010 in Seoul
    • Place of Presentation
      Seoul COEX (Seoul, Korea)
    • Related Report
      2012 Final Research Report
  • [Presentation] 顎骨腫瘍2010

    • Author(s)
      程 〓,常木雅之,丸山 智,山崎 学,金丸祥平,新垣 晋,齊藤 力,西山秀昌,林 孝文,朔 敬
    • Organizer
      第20回日本口腔粘膜病学会・第21回日本臨床口腔病理学会総会・学術大会
    • Place of Presentation
      大阪歯科大学楠葉学舎 (大阪府枚方市)
    • Related Report
      2012 Final Research Report
  • [Presentation] 角化嚢胞性歯原性腫瘍におけるパールカン結合分子2010

    • Author(s)
      長谷川真弓,程 〓,丸山 智,山崎学,齊藤 力,朔
    • Organizer
      第20回日本口腔粘膜病学会・第21回日本臨床口腔病理学会総会・学術大会
    • Place of Presentation
      大阪歯科大学楠葉学舎(大阪府枚方市)
    • Related Report
      2012 Final Research Report
  • [Presentation] 角化嚢胞性歯原性腫瘍におけるパールカン結合因子2010

    • Author(s)
      長谷川真弓
    • Organizer
      第20回日本口腔粘膜病学会・第21回日本臨床口腔病理学会
    • Place of Presentation
      枚方市
    • Related Report
      2010 Annual Research Report
  • [Presentation] 顎骨腫瘍2010

    • Author(s)
      程瑞
    • Organizer
      第20回日本口腔粘膜病学会・第21回日本臨床口腔病理学会
    • Place of Presentation
      枚方市
    • Related Report
      2010 Annual Research Report
  • [Presentation] Combined immunohistochemistry for the differential diagnosis of cystic jaw lesions: its practical use in surgical pathology2010

    • Author(s)
      Tsuneki M
    • Organizer
      15th Biennial Congress of the International Association of Oral Pathologists
    • Place of Presentation
      Seoul, South Korea
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.dent.niigata-u.ac.jp/patho/publications/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.dent.niigata-u.ac.jp/pahto/publications/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.dent.niigata-u.ac.jp/patho/publications/index.html

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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