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Transcriptional and post-transcriptional regulation of noradrenaline transporter gene expression by nicotine

Research Project

Project/Area Number 22592066
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionOkayama University

Principal Investigator

OHYAMA Kazumi  岡山大学, 大学院・医歯薬学総合研究科, 助手 (00253021)

Co-Investigator(Kenkyū-buntansha) KITAYAMA Shigeo  岡山大学, 大学院・医歯薬学総合研究科, 教授 (80177873)
SOGAWA Norio  岡山大学, 大学院・医歯薬学総合研究科, 准教授 (30236153)
SOGAWA Chiharu  岡山大学, 大学院・医歯薬学総合研究科, 助教 (10253022)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords歯科薬理学 / ニコチン / 薬理学 / 脳・神経 / 発現制御 / シグナル伝達 / トランスポーター / ノルアドレナリン / 鎮痛 / 転写 / 翻訳
Research Abstract

We investigated the effects of nicotine on noradrenaline transporter(NET) expression in PC12 cells derived from rat pheochromocytoma, human neuroblastoma SK-N-SH cells, and rat tissues. Nicotine had no influence on the transcriptional activity of human NET gene constructs transiently transfected in SK-N-SH and PC12 cells. However, nicotine decreased NET mRNA levels within several hours in SK-N-SH cells. In contrast, nicotine up-regulated the mRNA and protein of NET in PC12 cells. A single administration of nicotine increased NET mRNA levels in the adrenal medulla and brain stem of the rat, whereas chronic nicotine treatment for 2 weeks decreased NET mRNA levels. These results suggest that nicotine up- and down-regulates the expression of NET through a posttranscriptional mechanism. The decrease in NET expression induced by nicotine may be ofparticular clinical relevance to the pharmacological action of nicotine.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (26 results)

All 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (13 results) Book (4 results)

  • [Journal Article] Protective effect of cepharanthin on cisplatin-induced renal toxicity through metallothioneinexpression2013

    • Author(s)
      Sogawa N, Hirai K, Sogawa C, Ohyama K, Miyazaki I, Tsukamoto G, Asanuma M, Sasaki A, Kitayama S
    • Journal Title

      Life Sciences

      Pages: 727-732

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protective effect of cepharanthin on cisplatin-induced renal toxicity through metallothionein expression.2013

    • Author(s)
      Sogawa N, Hirai K, Sogawa C, Ohyama K, Miyazaki I, Tsukamoto G, Asanuma M, Sasaki A, Kitayama S.
    • Journal Title

      Life Sciences

      Volume: 92 Pages: 727-732

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Age-dependent changes in the susceptibility to thiopental anesthesia in mice: analysis of the relationship to the functional expression of GABA transporter, Pharmacology2012

    • Author(s)
      Sogawa N, Hazehara Y, Kunitomo M, Morita Y, Yoo B, Ohyama K, Sogawa C, Kitayama S
    • Journal Title

      Biochemistry and Behavior

      Pages: 267-272

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Age-dependent changes in the susceptibility to thiopental anesthesia in mice: analysis of the relationship to the functional expression of GABA transporter,2012

    • Author(s)
      Sogawa N, Hazehara Y, Kunitomo M, Morita Y, Yoo B, Ohyama K, Sogawa C, Kitayama S.
    • Journal Title

      Pharmacology, Biochemistry and Behavior

      Volume: 103 Pages: 267-272

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methylone and monoamine trasupoters : Correlation with toxicity2011

    • Author(s)
      C.Sogawa, et al
    • Journal Title

      Current Neuropharmacology

      Volume: 9 Pages: 58-62

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nicotine stimulates transcriptional activity of the human dopamine transpoter gene2010

    • Author(s)
      K.Ohyama
    • Journal Title

      Neurosci

      Volume: 471 Pages: 34-37

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression and function of variants of human catecholamine transpoters lacking the fifthe transmembrane region encoded by exon62010

    • Author(s)
      C. Sogawa
    • Journal Title

      PLoS ONE

      Volume: 5

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nicotine stimulates transcriptional activity of the human dopamine transporter gene.2010

    • Author(s)
      K.Ohyama, et al.
    • Journal Title

      Neurosci Lett.

      Volume: 471 Pages: 34-37

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression and function of variants of human catecholamine transporters lacking the fifth transmembrane region encoded by2010

    • Author(s)
      C.Sogawa, et al.
    • Journal Title

      PLoS ONE

      Volume: 5

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Characterization of human noradrenaline transporter gene at 5'-flanking region2013

    • Author(s)
      Chiharu Sogawa, Kazumi Ohyama, Norio Sogawa, Shigeo Kitayama
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡市
    • Year and Date
      2013-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] Post-transcriptional regulation of noradrenaline transporter by nicotine2013

    • Author(s)
      Kazumi Ohyama, Chiharu Sogawa, Xue-fang Wen, Katsuya Morita, Norio Sogawa, Shigeo Kitayama
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡市
    • Year and Date
      2013-03-23
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Characterization of human noradrenaline transporter gene at 5’-flanking region2013

    • Author(s)
      Chiharu Sogawa, Kazumi Ohyama, Norio Sogawa, Shigeo Kitayama
    • Organizer
      第86回日本薬理学会年会
    • Place of Presentation
      福岡市
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒスタミンH3受容体によるノルアドレナリントランスポーターの機能および発現抑制2012

    • Author(s)
      十川紀夫,十川千春,文 学 方 ,大山和美,秦泉寺紋子,宮脇卓也,北山滋雄
    • Organizer
      第33回岡山歯学会
    • Place of Presentation
      岡山市
    • Year and Date
      2012-11-25
    • Related Report
      2012 Final Research Report
  • [Presentation] ニコチンによるノルアドレナリントランスポーター発現の調節2012

    • Author(s)
      大山和美,十川千春,文学方,森田克也,十川紀夫,北山滋雄
    • Organizer
      第122回近畿部会、第122回近畿部会
    • Place of Presentation
      豊中市
    • Year and Date
      2012-11-16
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒトノルアドレナリントランスポーター遺伝子5'プロモーター領域の解析2012

    • Author(s)
      北山滋雄,十川千春,大山和美,十川紀夫
    • Organizer
      第122回近畿部会
    • Place of Presentation
      豊中市
    • Year and Date
      2012-11-06
    • Related Report
      2012 Final Research Report
  • [Presentation] Nicotine regulation of the expression of noradrenaline transporter gene2012

    • Author(s)
      Chiharu Sogawa, Kazumi Ohyama, Xue-fang Wen, Norio Sogawa, Shigeo Kitayama
    • Organizer
      The 11th Biennial Meeting of Asian-Pacific Society for Neurochemistry 55th Annual Meeting of the Japanese Society for Neurochemistry
    • Place of Presentation
      神戸市
    • Year and Date
      2012-10-02
    • Related Report
      2012 Final Research Report
  • [Presentation] Nicotine regulation of the expression of noradrenaline transporter gene2012

    • Author(s)
      Chiharu Sogawa, Kazumi Ohyama, Xue-fang Wen, Norio Sogawa, Shigeo Kitayama
    • Organizer
      The 11th Biennial Meeting of Asian-Pacific Society for Neurochemistry 55th Annual Meeting of the Japanese Society for Neurochemistry
    • Place of Presentation
      神戸市
    • Related Report
      2012 Annual Research Report
  • [Presentation] Transcriptional and post-transcriptional regulation of the dopamine and norepinephrine transpoters by nicotine2010

    • Author(s)
      S.Kitayama
    • Organizer
      NeuroTalk2010
    • Place of Presentation
      Singapore
    • Year and Date
      2010-06-28
    • Related Report
      2012 Final Research Report
  • [Presentation] Transcriptional and post-transcriptional regulation of the dopamine and norepinephrine transpoters by nicotine.2010

    • Author(s)
      S.Kitayama
    • Organizer
      NeuroTalk2010
    • Place of Presentation
      Singapore
    • Related Report
      2010 Annual Research Report
  • [Presentation] ヒトノルアドレナリントランスポーター遺伝子5’プロモーター領域の解析

    • Author(s)
      北山滋雄,十川千春,大山和美,十川紀夫
    • Organizer
      第122回近畿部会
    • Place of Presentation
      豊中市
    • Related Report
      2012 Annual Research Report
  • [Presentation] ニコチンによるノルアドレナリントランスポーター発現の調節

    • Author(s)
      大山和美,十川千春,文学方,森田克也,十川紀夫,北山滋雄
    • Organizer
      第122回近畿部会
    • Place of Presentation
      豊中市
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒスタミンH3受容体によるノルアドレナリントランスポーターの機能および発現抑制

    • Author(s)
      十川紀夫,十川千春,文学方,大山和美,秦泉寺紋子,宮脇卓也,北山滋雄
    • Organizer
      第33回岡山歯学会
    • Place of Presentation
      岡山市
    • Related Report
      2012 Annual Research Report
  • [Book] Immunocytochemical approaches to the identification of membrane topology of the Na+/Cl- -dependent neurotransmitter transporters2012

    • Author(s)
      Sogawa C, Sogawa N, Kitayama S
    • Total Pages
      17
    • Publisher
      Applications of Immunocytochemistry
    • Related Report
      2012 Final Research Report
  • [Book] Molecular and Genetic Basis of the Regulation of Dopamine Transporter. Dopamine: Functions, Regulation and Health Effects2012

    • Author(s)
      Kitayama S, Sogawa C, Sogawa N
    • Total Pages
      27
    • Publisher
      Nova Science、Publishers
    • Related Report
      2012 Final Research Report
  • [Book] Applications of Immunocytochemistry.2012

    • Author(s)
      Sogawa C, Sogawa N, Kitayama S,
    • Total Pages
      17
    • Publisher
      Immunocytochemical approaches to the identification of membrane topology of the Na+/Cl- -dependent neurotransmitter transporters.
    • Related Report
      2012 Annual Research Report
  • [Book] Publishers2012

    • Author(s)
      Kitayama S, Sogawa C, Sogawa N, Molecular and Genetic Basis of the Regulation of Dopamine Transporter.
    • Total Pages
      27
    • Publisher
      Dopamine: Functions, Regulation and Health Effects. Nova Science
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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