Transcriptional and post-transcriptional regulation of noradrenaline transporter gene expression by nicotine
| Project/Area Number |
22592066
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
OHYAMA Kazumi 岡山大学, 大学院・医歯薬学総合研究科, 助手 (00253021)
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| Co-Investigator(Kenkyū-buntansha) |
KITAYAMA Shigeo 岡山大学, 大学院・医歯薬学総合研究科, 教授 (80177873)
SOGAWA Norio 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (30236153)
SOGAWA Chiharu 岡山大学, 大学院・医歯薬学総合研究科, 助教 (10253022)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 歯科薬理学 / ニコチン / 薬理学 / 脳・神経 / 発現制御 / シグナル伝達 / トランスポーター / ノルアドレナリン / 鎮痛 / 転写 / 翻訳 |
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| Research Abstract |
We investigated the effects of nicotine on noradrenaline transporter(NET) expression in PC12 cells derived from rat pheochromocytoma, human neuroblastoma SK-N-SH cells, and rat tissues. Nicotine had no influence on the transcriptional activity of human NET gene constructs transiently transfected in SK-N-SH and PC12 cells. However, nicotine decreased NET mRNA levels within several hours in SK-N-SH cells. In contrast, nicotine up-regulated the mRNA and protein of NET in PC12 cells. A single administration of nicotine increased NET mRNA levels in the adrenal medulla and brain stem of the rat, whereas chronic nicotine treatment for 2 weeks decreased NET mRNA levels. These results suggest that nicotine up- and down-regulates the expression of NET through a posttranscriptional mechanism. The decrease in NET expression induced by nicotine may be ofparticular clinical relevance to the pharmacological action of nicotine.
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Report
(4 results)
Research Products
(26 results)
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[Book] Applications of Immunocytochemistry.2012
Author(s)
Sogawa C, Sogawa N, Kitayama S,
Total Pages
17
Publisher
Immunocytochemical approaches to the identification of membrane topology of the Na+/Cl- -dependent neurotransmitter transporters.
Related Report
-
[Book] Publishers2012
Author(s)
Kitayama S, Sogawa C, Sogawa N, Molecular and Genetic Basis of the Regulation of Dopamine Transporter.
Total Pages
27
Publisher
Dopamine: Functions, Regulation and Health Effects. Nova Science
Related Report
