Involvement of Dec2 in regulation of chondrogenic differentiation via interaction with Sox proteins

• Project/Area Number: 22592067
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Hiroshima University
• Principal Investigator: FUJIMOTO Katsumi 広島大学, 医歯薬保健学研究院, 助教 (40294566)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 口腔生化学 / 軟骨分化 / 転写因子

Research Abstract

I studied about the role of Dec2 in regulating chondrogenic differentiation of mesenchymal stem cells (MSCs). Overexpression of Dec2 decreased the expressions of proteoglycan and chondrocyte-related maker genes in chondrogenic-induced MSCs, suggesting that Dec2 negatively regulates chondrogenic differentiation. Dec2 also suppressed proliferation of MSCs in high-density pellet culture by regulating the expression of cell cycle-related genes such as cyclin D, p21 and p16.

Research Products

• [Journal Article] The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer (2012)
  • Author(s): Wu, Y., Kawamoto, T., Noshiro, M., et al.(他10名)
  • Journal Title: Int J Oncol Volume: 41 Issue: 4 Pages: 1337-1346
  • DOI: 10.3892/ijo.2012.1559
  • Peer Reviewed
• [Journal Article] Identification of a new clock-related element EL-box involved in circadian regulation by BMAL1/CLOCK and HES1 (2012)
  • Author(s): Ueshima, T., Kawamoto, T., Honda, K.K, Noshiro, M., Fujimoto, K., Nakao, S., Ichinose, N., Hashimoto, S., Gotoh, O., Kato, Y
  • Journal Title: Gene Volume: 510 (2) Issue: 2 Pages: 118-125
  • DOI: 10.1016/j.gene.2012.08.022
  • Peer Reviewed
• [Journal Article] The basic helix-loop-helix transcription factor DEC2 inhibits TGF-beta-induced tumor progression in human pancreatic cancer BxPC-3 cells (2012)
  • Author(s): Sato, F., Kawamura, H., Kawamoto, T., Noshiro, M.,(他10名)
  • Journal Title: Int J Mol Med Volume: 30 Issue: 3 Pages: 495-501
  • DOI: 10.3892/ijmm.2012.1037
  • Peer Reviewed
• [Journal Article] IL-1β-mediated up-regulation of DEC1 in human gingiva cells via the Akt (2012)
  • Author(s): Bhawal UK, Ito Y, Tanimoto K, Sato F, Fujimoto K, Kawamoto T, Sasahira T, Hamada N, Kuniyasu H, Arakawa H, Kato Y
  • Journal Title: J Cell Biochem Volume: Vol113 Issue: 10 Pages: 3246-53
  • DOI: 10.1002/jcb.24205
  • Peer Reviewed
• [Journal Article] BHLH transcription factor DEC2 regulates pro-apoptotic factor Bim in human oral cancer HSC-3 cells (2012)
  • Author(s): Wu, Y., Sato, F., Bhawal, U.K., Kawamoto, T., Fujimoto, K., Noshiro, M., Seino, H., Morohashi, S., Kato, Y., Kijima, H
  • Journal Title: Biomedical Research Volume: 33 Issue: 2 Pages: 75-82
  • DOI: 10.2220/biomedres.33.75
  • NAID: 130004470811
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed
• [Journal Article] Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver (2012)
  • Author(s): Sato, F., Wu, Y., Noshiro, M.
  • Journal Title: Biochem Biophys Res Commun Volume: 419 Issue: 2 Pages: 441-446
  • DOI: 10.1016/j.bbrc.2012.02.076
  • Peer Reviewed
• [Journal Article] Regulation of basic helix-loop-helix transcription factors Dec1 and Dec2 by RORα and their roles in adipogenesis. (2012)
  • Author(s): Ozaki N
  • Journal Title: Genes to Cells Volume: 17 Issue: 2 Pages: 107-121
  • DOI: 10.1111/j.1365-2443.2011.01574.x
  • Peer Reviewed
• [Journal Article] Time-dependent interaction between differentiated embryo chondrocyte-2 and CCAAT/enhancer-binding protein αunderlies the circadian expression of CYP2D6 in serum-shocked HepG2 cells (2012)
  • Author(s): Matsunaga, N., Inoue, M., Kusunose, N., Kakimoto, K., Hamamura, K., Hanada, Y., Toi, A., Yoshiyama, Y., Sato, F., Fujimoto, K., Koyanagi, S., Ohdo, S
  • Journal Title: Mol. Pharmacol Volume: 81(5) Issue: 5 Pages: 739-747
  • DOI: 10.1124/mol.111.076406
  • Peer Reviewed
• [Journal Article] 循環器疾患における時計遺伝子DEC1の意義 (2012)
  • Author(s): 加藤幸夫、中島歩、河本健、藤本勝巳、能城光秀
  • Journal Title: Heart View Volume: 16(8) Pages: 103-107
• [Journal Article] 無血清で増幅した間葉系幹細胞と歯髄細胞 (2012)
  • Author(s): 加藤幸夫、藤井紗貴子、五藤紀子、北山和子、金輪真佐美、河本健、藤本勝巳、邵金昌、桂由紀、藩海鴎、長谷川森一、辻紘一郎
  • Journal Title: 広島歯科医学雑誌 Volume: 39 Pages: 1-8
• [Journal Article] Basic helix-loop-helix transcription factor DEC1 negatively regulates cyclin D1. Bhawal (2011)
  • Author(s): U.K., Sato, F., Arakawa, Y., Fujimoto, K., Kawamoto, T., Tanimoto, K., Ito, Y., Sasahira, T., Sakurai, T., Kobayashi, M., Kashima, I., Kijima, H., Kuniyasu, H., Abiko, Y., Kato, Y., Sato, S
  • Journal Title: J. Pathol Volume: 224(3) Issue: 3 Pages: 420-429
  • DOI: 10.1002/path.2878
  • Peer Reviewed
• [Journal Article] Basic helix-loop-helix transcription factors DEC1 and DEC2 regulate the paclitaxel-induced apoptotic pathway of MCF-7 human breast cancer cells (2011)
  • Author(s): Wu, Y., Sato, F., Kawamoto, T., Fujimoto, K., Noshiro, M., (他4名)
  • Journal Title: Int J Mol Med Volume: 27 Issue: 4 Pages: 491-495
  • DOI: 10.3892/ijmm.2011.617
  • Peer Reviewed
• [Journal Article] 無血清で増幅した間葉系幹細胞と歯髄細胞 (2011)
  • Author(s): 加藤幸夫, ら
  • Journal Title: 広島歯科医学雑誌 Volume: 39 Pages: 1-8
• [Journal Article] Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells (2010)
  • Author(s): Liu, Y., Sato, F., Kawamoto, T., Noshiro, M.
  • Journal Title: Genes Cells Volume: 15 Issue: 4 Pages: 315-325
  • DOI: 10.1111/j.1365-2443.2010.01381.x
  • Peer Reviewed
• [Journal Article] Interleukin-4 up-regulates histamine H1 receptors by activation of H1 receptor gene transcription (2010)
  • Author(s): Horio S, Fujimoto K, Mizuguchi H, Fukui H
  • Journal Title: Naunyn Schmiedebergs Arch Pharmacol Volume: 381 Issue: 4 Pages: 305-313
  • DOI: 10.1007/s00210-010-0491-z
  • Peer Reviewed
• [Journal Article] Roles of DEC1 and DEC2 in the core loop of the circadian clock, and clock outputs to metabolism (2010)
  • Author(s): Kato Y, Noshiro M, Fujimoto K, Kawamoto T
  • Journal Title: Hirosaki Med. J Volume: 61(Suppl.)
• [Journal Article] Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells. (2010)
  • Author(s): Yang Liu, et al.
  • Journal Title: Genes to cells Volume: 15・4 Pages: 315-325
  • Peer Reviewed
• [Journal Article] Interleukin-4 up-regulates histamine H1 receptors by activation of H1 receptor gene transcription (2010)
  • Author(s): Shuhei Horio, et al.
  • Journal Title: Naunyn-Schmiedeberg's Archives of Pharmacology Volume: 381・4 Pages: 305-313
  • Peer Reviewed
• [Journal Article] Roles of DEC1 and DEC2 in the core loop of the circadian clock, and clock outputs to metabolism. (2010)
  • Author(s): Yukio Kato, et al.
  • Journal Title: Hirosaki Med.J. Volume: 61(Suppl.)
• [Presentation] Enhanced Proliferation of Stem Cells from Deciduous Teeth in Serum-free Media: STK1/STK2 (2012)
  • Author(s): Noriko Goto, Katsumi Fujimoto, Shin-ichi Hasegawa, Kazuko Kitayama, Veronica Sainik Ronald, Katsuyuki Kozai, Yukio Kato
  • Organizer: 8th Biennial Conference PDAA (Pediatric Dentistry Association of Asia)
  • Place of Presentation: Bali Indonesia
• [Presentation] 概日リズムを制御する新規時計エレメント EL-box の同定 (2012)
  • Author(s): 河本健、能城光秀、藤本勝巳、加藤幸夫
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山市
• [Presentation] Identification of marker genes expressed in human dental pulp cells (2012)
  • Author(s): Fujii S., Fujimoto K., Srivatanakul P., Nishimura F., Kato Y.
  • Organizer: 3rd Asian Cellular Therapy Organization meeting
  • Place of Presentation: Chiang Mai Thailand
• [Presentation] ヒト乳歯歯髄細胞における転写因子 MSX1の標的遺伝子の網羅的解析 (2012)
  • Author(s): 五藤紀子、藤本勝巳、 藤井紗貴子、香西克之、加藤幸夫
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡市
• [Presentation] Enhanced Proliferation of Stem Cells from Deciduous Teeth in Serum-free Media, STK1/STK2. (2012)
  • Author(s): Goto N
  • Organizer: 8th Biennial Conference PDAA (Pediatric Dentistry Association of Asia)
• [Presentation] 概日リズムを制御する新規時計エレメントEL-boxの同定 (2012)
  • Author(s): 河本健
  • Organizer: 第54回歯科基礎医学会学術大会・総会
• [Presentation] Identification of marker genes expressed in human dental pulp cells. (2012)
  • Author(s): Fujii S
  • Organizer: 3rd Asian Cellular Therapy Organization meeting
• [Presentation] ヒト乳歯歯髄細胞における転写因子MSX1の標的遺伝子の網羅的解析 (2012)
  • Author(s): 五藤紀子
  • Organizer: 第85回日本生化学会大会
• [Presentation] 無血清培地STK2 によるヒト歯髄細胞の増殖および石灰化能への影響 (2011)
  • Author(s): 藤井紗貴子、藤本勝巳、西村英紀、加藤幸夫
  • Organizer: 第50回広島県歯科医学会第95回広島大学歯学会
  • Place of Presentation: 広島市
  • Year and Date: 2011-11-13
• [Presentation] 無血清培地STK2によるヒト歯髄細胞の増殖および石灰化能への影響 (2011)
  • Author(s): 藤井紗貴子, 他
  • Organizer: 第50回広島県歯科医学会第95回広島大学歯学会
  • Place of Presentation: 広島市
  • Year and Date: 2011-11-13
• [Presentation] Transcription factors regulating differentiation of MSC (2011)
  • Author(s): Kawamoto T., Kubo H., Iwata Y., Fujimoto K., Noshiro M., Kato Y.
  • Organizer: 3rdInternational Workshop on BioDental Education & Research Hiroshima University Graduate School of Biomedical Sciences
  • Place of Presentation: 広島市
• [Presentation] Expression profiles of nuclear receptors and their functions in rat growth plate Chondrocytes (2011)
  • Author(s): Ozaki N., Noshiro M., Honda K., Hayashibara K., Kawamoto T., Fujimoto K., Tanne K., Kato Y
  • Organizer: Ozaki N., Noshiro M., Honda K., Hayashibara K., Kawamoto T., Fujimoto K., Tanne K., Kato Y
  • Place of Presentation: 広島市
• [Presentation] Effect of conditioned medium derived from mouse ameloblast lineage cells on differentiation of human dental pulp cells (2011)
  • Author(s): Fujii S., Fujimoto K., Nishimura F., Kato Y.
  • Organizer: 3rdInternational Workshop on BioDental Education & Research Hiroshima University Graduate School of Biomedical Sciences
  • Place of Presentation: 広島市
• [Presentation] 細胞表面抗原を指標とした口腔内からの骨前駆細胞の分取 (2011)
  • Author(s): 鎌田浩一、藤本勝巳、西村正宏、末廣史雄、貞森紳丞、赤川安正、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
• [Presentation] 間葉系幹細胞の軟骨分化におけるDEC2 の役割 (2011)
  • Author(s): 笹本智子、藤本勝巳、金輪真佐美、河本健、能城光秀、道田将彦、尾崎徳継、丹根一夫、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
• [Presentation] 間葉系幹細胞の増殖・分化ならびにインテクリン発現に対する転写因子 GATA6 の作用 (2011)
  • Author(s): 道田将彦、河本健、能城光秀、藤本勝巳、金輪真佐美、尾崎徳継、笹本智子、丹根一夫、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
• [Presentation] 核内受容体 RORαによる時計遺伝子DEC1,DEC2 の発現調節と脂肪分化 (2011)
  • Author(s): 尾崎徳継、能城光秀、河本健、福崎麗、藤本勝巳、丹根一夫、加藤幸夫
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
• [Presentation] 間葉系幹細胞の軟骨分化におけるDEC2の役割 (2011)
  • Author(s): 笹本智子, 他
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
• [Presentation] 間葉系幹細胞の増殖・分化ならびにインテクリン発現に対する転写因子GATA6の作用 (2011)
  • Author(s): 道田将彦, 他
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
• [Presentation] 核内受容体RORαによる時計遺伝子DEC1,DEC2の発現調節と脂肪分化 (2011)
  • Author(s): 尾崎徳継, 他
  • Organizer: 第52回日本生化学会中国・四国支部例会
  • Place of Presentation: 広島市
• [Presentation] 核内受容体(RORα) による時計遺伝子(Dec1, Dec2) の制御 (2010)
  • Author(s): 尾崎徳継、能城光秀、河本健、藤本勝巳、丹根一夫、加藤幸夫
  • Organizer: 第51回日本生化学会中国・四国支部例会
  • Place of Presentation: 山口市
• [Presentation] 口腔組織からの骨前駆細胞の分取:細胞表面抗原による解析 (2010)
  • Author(s): 鎌田 浩一、藤本 勝巳、西村 正宏、貞森 紳丞、赤川 安正、加藤 幸夫
  • Organizer: 第51回日本生化学会中国・四国支部例会
  • Place of Presentation: 山口市
• [Presentation] 核内受容体(RORa)による時計遺伝子(Dec1, Dec2)の制御 (2010)
  • Author(s): 尾崎徳継
  • Organizer: 第51回日本生化学会中国・四国支部例会
  • Place of Presentation: 山口市
• [Patent(Industrial Property Rights)] 脂質代謝関連疾患の検査方法、ならびに脂質代謝関連疾患の予防および/または治療剤の評価方法 (2011)
  • Inventor(s): 加藤幸夫、河本健、藤本勝巳、能城光秀
  • Industrial Property Rights Holder: 広島大学
  • Industrial Property Number: 2011-022172
  • Filing Date: 2011-02-03
• [Patent(Industrial Property Rights)] 脂質代樹関連疾患の検査方法、ならびに脂質代謝関連疾患の予防および/または治療剤の評価方法 (2011)
  • Inventor(s): 加藤幸夫、河本健、藤本勝巳、能城光秀
  • Industrial Property Rights Holder: 広島大学
  • Industrial Property Number: 2011-022172
  • Filing Date: 2011-02-03