The regulation of bone mass by calcium influx propertiesof osteoclasts
| Project/Area Number |
22592071
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KOMORI Toshihisa 長崎大学, 医歯薬学総合研究科, 教授 (00252677)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | カルシウムチャンネル / 破骨細胞 / カルシウムシグナル / カルシウム / 骨代謝 / TRPV4 |
|---|
| Research Abstract |
Osteoclast differentiation is critically dependent on calcium (Ca2+) signaling. Transient receptor potential vaniroid (TRPV) 4, mediates Ca2+ influx in the late stage of osteoclast differentiation, thereby regulates Ca2+ signaling. However, the system-modifying effect of TRPV4 activity remains to be determined. Bone loss due to TRPV4 activation was abrogated by loss of interactions between Ca2+/calmodulin signaling and TRPV4. Finally, modulators of TRPV4 interactions with the calmodulin-binding domain were investigated by proteomic analysis. Non-muscle myosin IIa was then identified by LC-MS/MS analysis, which was confirmed by immunoblotting following coimmunoprecipitation with TRPV4. These results indicate that TRPV4 activation reciprocally regulates Ca2+/calmodulin signaling, which involves an association of TRPV4 with myosin IIa, and promotes sufficient osteoclast function.
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Report
(4 results)
Research Products
(14 results)
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[Journal Article] Pyruvate dehydrogenase kinase 4 induces bone loss at unloading by promoting osteoclastogenesis.2012
Author(s)
Wang Y, Liu W, Masuyama R, Fukuyama R, Ito M, Zhang Q, Komori H, Murakami T, Moriishi T, Miyazaki T, Kitazawa R, Yoshida CA, Kawai Y, Izumi S, Komori T
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Journal Title
Bone
Volume: vol50
Pages: 409-419
Related Report
Peer Reviewed
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[Journal Article] Normocalcemia is maintained in mice under conditions of calcium malabsorption by vitamin D-induced inhibition of bone mineralization2012
Author(s)
Lieben L, Masuyama R, Torrekens S, Van Looveren R, Schrooten J, Baatsen P, Lafage-Proust MH, Dresselaers T, Feng JQ, Bonewald LF, Meyer MB, Pike JW, Bouillon R, Carmeliet G
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Journal Title
Journal of Clinical Investigation
Volume: vol122
Pages: 1803-1815
Related Report
Peer Reviewed
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[Journal Article] Overexpression of Bcl2 in osteoblasts inhibits osteoblast differentiation and induces osteocyte apoptosis2011
Author(s)
Moriishi T, Maruyama Z, Fukuyama R, Ito M, Miyazaki T, Kitaura H, Ohnishi H, Furuichi T, Kawai Y, Masuyama R, Komori H, Takada K, Kawaguchi H, Komori T
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Journal Title
NAID
Related Report
Peer Reviewed
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