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StudyofintracellularlocalizationofIP3-receptortype2

Research Project

Project/Area Number 22592072
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionKyushu Dental College

Principal Investigator

MASUDA Wataru  九州歯科大学, 歯学部, 助教 (80295865)

Co-Investigator(Kenkyū-buntansha) JIMI Eijirou  九州歯科大学, 歯学部, 教授 (40276598)
Co-Investigator(Renkei-kenkyūsha) FUKUSHIMA Hidefumi  九州歯科大学, 歯学部, 講師 (70412624)
Research Collaborator D.I. Yule  米国ロチェスター大学, 教授
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
KeywordsIP3 受容体 / IRAG / 細胞内局在 / IP3受容体 / 唾液腺 / IP_3受容体type2
Research Abstract

IP3R type I (IP3R-I) was phosphorylated by PKA and this causesthe potentiation of carbachol (CCh)-induced Ca-release from IP3R-I. In the presence ofIRAG and PKG Iβ, IP3R-I forms a complex with these molecules and this causes theloss of both the phosphorylation by PKA and the potentiation of CCh-inducedCa-release. IP3R type II (IP3R-II) also be able to form a complex with IRAG and PKG Iβ. However, the phosphorylation of IP3R-II by PKA and the potentiation of carbachol(CCh)-induced Ca-release from IP3R-II were not inhibited by forming a complex. Inthis study, we identified the regions of IP3R molecules, which were required forinteraction with IRAG. In the IP3R-I, the region was much close to the phosphorylationsite, while it was far from the phosphorylation site in the IP3R-II. This result indicatedthat the interaction of IP3R-I with IRAG spatially did not permit PKA to access tophosphorylation site.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (8 results)

All 2012 2011 2010

All Journal Article (2 results) (of which Peer Reviewed: 1 results) Presentation (6 results)

  • [Journal Article] InsP3R-associated cGMP kinasesubstrate (IRAG) determines inositol1,4,5-trisphosphate receptors susceptibilityto phosphoregulation by cyclic nucleotidedependent kinases2010

    • Author(s)
      Masuda W., Betzenhauser M.J., YuleD.I.
    • Journal Title

      J. Biol. Chem

      Volume: 285 Pages: 37927-37938

    • Related Report
      2012 Final Research Report
  • [Journal Article] InsP3R-associated cGMP kinase substrate(IRAG)determines inositol 1, 4, 5-trisphosphate receptors susceptibility to phosphoregulation by cyclic nucleotide dependent kinases2010

    • Author(s)
      Masuda W., Betzenhauser M.J., Yule D.I.
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 37927-37938

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] IP3R 分子内におけるIRAG 結合部位2012

    • Author(s)
      増田渉, 福島秀文, 自見英治郎
    • Organizer
      第54回歯科基礎医学会
    • Place of Presentation
      郡山
    • Related Report
      2012 Final Research Report
  • [Presentation] IP3R分子内におけるIRAG結合領域2012

    • Author(s)
      増田 渉、福島秀文、自見英治郎
    • Organizer
      歯科基礎医学会学術大会
    • Place of Presentation
      奥羽大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] IP3R 分子内におけるIRAG 結合領域の同定2011

    • Author(s)
      増田渉, 自見英治郎
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] IP3R分子内におけるIRAG結合領域の同定2011

    • Author(s)
      増田渉、自見英治郎
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Related Report
      2011 Annual Research Report
  • [Presentation] IP3R /IRAG/PKG Iβ複合体におけるCa2+放出機構2010

    • Author(s)
      増田渉, Betzenhauser M.J., Yule D.I.
    • Organizer
      第33回日本分子生物学会0会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-01-20
    • Related Report
      2012 Final Research Report
  • [Presentation] IP3R/IRAG/PKG1β複合体におけるCa2+放出機構2010

    • Author(s)
      増田渉、Betzenhauser M.J., Yule D.I.
    • Organizer
      第33回日本分子生物学会年会・第83回に本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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