| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Stim1-mKO1 was expressed in rat submandibular glands by retrograde ductal injection of an adenoviral vector. Stim1-mKO1 proteins were expressed predominantly in acinar cells following infection with an adenoviral vector. Confocal microscopy and Ca^ imaging analysis indicated that the Stim1-mKO1 expressed in submandibular acinar cells is functional. The overexpression of Stim1-mKO1 enhanced the agonist-induced Ca^ release from intracellular Ca^ stores and the Ca^ entry through store-operated Ca^ entry. It is possible that the overexpression of Stim1 increased salivary fluid secretion compared to the control. In addition, we succeeded in monitoring the agonist-induced Ca^ dynamics in SMG in live animals using the Ca^ indicator, G-GECO.
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