| Project/Area Number |
22592078
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 歯周病 / P.gingivalis / ゲノムプロジェクト / プロテアーゼ / マイナー線毛 / 抗体 / P. gingivalis / ヘミン結合タンパク質 / HBP35 |
|---|
| Research Abstract |
Analyzing the genomic informationfor TDC60 is expected to provide new insights into the mechanisms underlying the onset and progression of periodontitis. For that reason, we searched for the specific virulence factor in the TDC60 strain using the complete genome sequence s,and found higher levels of adipeptidasePepD-2,a minorfibire-1(Mfa1),a collagenolysis-related peptidase Dap2 comparedto the W83 strain using 2-DE and MALD -TOF mass spectrometry. The crystal structure of PepD showed that the overall flding and dimeric organization. Bestatin has the ability to dose-dependentlyinhi it the P. gingivalis growth. Dipeptidases play a generalrole in the final break own of peptide fragments produced by other peptidases during the protein degradation process in P. gingivalis.
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