| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Approximately 50% decreased protein expression was observed for3’UTR of HIF-1 containing constructs compared to the control vector. In particular, a highly-aggressive breast cancer cell line MDA-MB-453 demonstrated a higher protein expression compared to a less-aggressive breast cancer cell line MCF7. It is plausible that 3’UTR dependent degradation of HIF-1a mRNA is repressed in several cancer cells which indicate malignant phenotypes. We also found the same phenotype in oral squamous cell carcinoma cells. Thus, 3’UTR dependent regulation of mRNA degradation might play an important role in the progression (vascular induction, invasion oreptherial-mesenchymal transition) of oral cancers.
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