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Organotypic epithelial raft cultures as HPV-related cancer models for evaluating siRNA and its delivery system

Research Project

Project/Area Number 22592084
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathobiological dentistry/Dental radiology
Research InstitutionUniversity of Tsukuba (2012)
Tokyo Medical and Dental University (2010-2011)

Principal Investigator

YAMATO Kenji  筑波大学, 医学医療系, 研究員 (50174751)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsHPV / RNAi / ラフトカルチャー / RNA干渉 / siRNA / 子宮頸癌 / GFP / 皮膚 / ルシフェラーゼ
Research Abstract

To utilize siRNA technology to the treatment of HPV-related diseases, developing an efficient siRNA delivery system is essential. In this study, we established organotypic raft cultures using TERT- and HPV16-E6E7 immortalized human keratinocytes stably expressing firefly luciferase. These cultures facilitated quantitative analysis of knock-down efficiency induced by local siRNA delivery and showed that some cationic liposomes could deliver siRNA through stratified squamous epithelium.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (14 results)

All 2012 2011 2010

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (10 results)

  • [Journal Article] The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome2012

    • Author(s)
      Egawa, N., et al
    • Journal Title

      J.Virol.

      Volume: 86 Issue: 6 Pages: 3276-3281

    • DOI

      10.1128/jvi.06450-11

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced specificity of HPV16 E6E7 siRNA by RNA-DNA chimera modification.2011

    • Author(s)
      Yamato K. et al.
    • Journal Title

      Cancer Gene Ther.

      Volume: 18 Issue: 8 Pages: 587-597

    • DOI

      10.1038/cgt.2011.28

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potent in vitro and in vivo antitumor effects of MDM2 inhibitor nutlin-3 in gastric cancer cells2011

    • Author(s)
      Endo, S., Yamato, K., Hirai, S., Moriwaki, M., Fukuda, K., Suzuki, H., Abei, M., Nakagawa, I. and Hyodo, I
    • Journal Title

      Cancer Sci

      Volume: 102 Pages: 605-613

    • NAID

      10029291334

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potent in vitro and in vivo antitumor effects of MDM2 inhibitor nutlin-3 in gastric cancer cells2011

    • Author(s)
      Endo, S.
    • Journal Title

      Cancer Sci.

      Volume: 102 Pages: 605-613

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Identification of a short RNA segment in the siRNA seed region required for efficient RNAi2012

    • Author(s)
      大和建嗣
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡 (福岡市)
    • Related Report
      2012 Final Research Report
  • [Presentation] ヒト胃癌細胞に対するclassIII HDAC阻害剤 tenovin-6の抗腫瘍効果に関する検討2012

    • Author(s)
      廣瀬充明
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ロイトイン札幌(札幌
    • Related Report
      2012 Final Research Report
  • [Presentation] The antitumor effect of HDAC inhibitor Tenovin-6 for gastric cancer cells2012

    • Author(s)
      Ueno T
    • Organizer
      American Association of Cancer Ressearch 103rd Annual Meeting
    • Place of Presentation
      Chicago, IL
    • Related Report
      2012 Final Research Report
  • [Presentation] Identification of a short RNA segment in the siRNA seed region required for efficient RNAi2012

    • Author(s)
      大和建嗣(代表)、遠藤慎治
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡 (福岡市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒト胃癌細胞に対するclassIII HDAC阻害剤 tenovin-6の抗腫瘍効果に関する検討2012

    • Author(s)
      廣瀬充明(代表)、遠藤慎治、齋藤梨絵、上野 卓教、鈴木英雄、大和 建嗣、兵頭一之介
    • Organizer
      第71回日本癌学会学術総会
    • Place of Presentation
      ロイトイン札幌(札幌)
    • Related Report
      2012 Annual Research Report
  • [Presentation] AGO2-association, microRNA suppression and cytotoxicity ofRNA-DNA chimera modified siRNA2011

    • Author(s)
      大和建嗣
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Related Report
      2012 Final Research Report
  • [Presentation] Enhanced specificity of HPV16 E6E7 siRNA by RNA-DNA chimera modification2011

    • Author(s)
      Yamato, K
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] Enhanced specificity of HPV16 E6E7 siRNA by RNA-DNA chimera modification.2010

    • Author(s)
      Yamato, K.
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] The anti-tumor effect of MDM2 antagonist nutlin-3 in gastric cancer cell in vitro and in vivo2010

    • Author(s)
      Endo S
    • Organizer
      American Association of Cancer Ressearch 101st Annual Meeting
    • Place of Presentation
      Washington, DC
    • Related Report
      2012 Final Research Report
  • [Presentation] The anti-tumorcancer effect of MDM2 antagonist nutlin-3 in gastric cancer cell in vitro and in vivo.2010

    • Author(s)
      Endo, S.
    • Organizer
      American Association of Cancer Research 101st Annual Meeting 2010
    • Place of Presentation
      Washington, DC
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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