Roles of Mitochondrial Reactive Oxygen Spesies in Suppression of Apoptosis Induced by X-irradiation
| Project/Area Number |
22592093
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Kagoshima University |
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Principal Investigator |
MAJIMA Hideyuki 鹿児島大学, 大学院・医歯学総合研究科, 教授 (60165701)
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| Co-Investigator(Kenkyū-buntansha) |
INDO Hiroko 鹿児島大学, 医歯学総合研究科, 助教 (00301391)
TOMITA Kazuo 鹿児島大学, 医歯学総合研究科, 助教 (60347094)
IWASHITA Youitirou 鹿児島大学, 医歯学総合研究科, 助教 (70168566)
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| Co-Investigator(Renkei-kenkyūsha) |
SUENAGA Shigeaki 鹿児島大学, 医学部・歯学部附属病院, 講師 (00136889)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 頭頸部放射線治療 / 口腔粘膜障害 / アポトーシス / 内因性酸化ストレス抑制療法 / がん / 放射線治療 / ミトコンドリア / 活性酸素 |
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| Research Abstract |
Human ells was transiently transfected with normal human MnSOD and MnSOD without a mitochondrial targeting signal (MTS). Mitochondrial reactive oxygen species (ROS), lipid peroxidation, and apoptosis were examined as a function of time following 18.8 Gy X-ray irradiation. The results showed that the level of mitochondrial ROS increased and reached a maximum level 2 h after X-ray irradiation. Authentic MnSOD, but not MnSOD lacking MTS, protected against mitochondrial ROS, lipid peroxidation, and apoptosis. In addition, the levels of mitochondrial ROS were consistently found to always correlate with the levels of authentic MnSOD in mitochondria. These results suggest that only when MnSOD is located in mitochondria is it efficient in protecting against cellular injuries by X-ray irradiation and that mitochondria are the critical sites of X-ray-induced cellular oxidative injuries.
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] Excellent Thermal Control Ability of Cell Biology Experiment Facility (CBEF) for Ground-Based Experiments and Experiments Onboard the Kibo Japanese Experiment Module of International Space Station2012
Author(s)
Yano S, Masuda D, Kasahara H, Omori K, Higashibata A, Asashima M, Ohnishi T, Yatagai F, Kamisaka S, Furusawa T, Higashitani A, Majima HJ, Nikawa T, Wakabayashi K, Takahashi H, Suzuki H, Shimazu T, Fukui K, Hattori A, Tanigaki F, Shirakawa M, Nakamura T, Yoshimura Y, Suzuki N, Ishioka N
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Journal Title
Biological Sciences in Space
Volume: 26 (1)
Pages: 12-20
URL
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[Journal Article] Roles of mitochondria-generated reactive oxygen species on X-ray-induced apoptosis in a human hepatocellular carcinoma cell line, HLE.2012
Author(s)
Indo HP, Inanami O, Koumura T, Suenaga S, Yen H-C, Kakinuma S, Matsumoto K, Nakanishi I, St Clair W, St Clair DK, Matsui H, Cornette R, Gusev O, Okuda T, Nakagawa Y, Ozawa T & Majima HJ
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Journal Title
Free Radical Research
Volume: Vol. 46, No. 8
Issue: 8
Pages: 1029-1043
DOI
Related Report
Peer Reviewed
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[Journal Article] Rebamipide attenuates nonsteroidal anti-inflammatory drugs (NSAID) induced lipid peroxidation by the manganese superoxide dismutase (MnSOD) overexpression in gastrointestinal epithelial cells2012
Author(s)
Nagano Y, Matsui H, Shimokawa O, Hirayama A, Tamura M, Nakamura Y, Kaneko T, Rai K, Indo HP, Majima HJ, Hyodo I
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Journal Title
J Physiol Pharmacol
Volume: 63(2)
Pages: 137-142
URL
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[Journal Article] Yen H-C, Li SH, Majima HJ, Huang YH, Chen CP, Liu CC, Tu YC, Chen CW2011
Author(s)
Yen H-C, Li SH, Majima HJ, Huang YH, Chen CP, Liu CC, Tu YC, Chen CW
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Journal Title
Free Radical Research
Volume: 45(6)
Issue: 6
Pages: 707-716
DOI
Related Report
Peer Reviewed
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[Journal Article] Lansoprazole inhibits mitochondrial superoxide production and cellular lipid peroxidation induced by indomethacin in RGM1 cells2011
Author(s)
Rai K, Matsui H, Kaneko T, Nagano Y, Shimokawa O, Udo J, Hirayama A, Hyodo I, Indo HP, Majima HJ
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Journal Title
Journal of Clinical Biochemistry and Nutrition
Volume: 49
Issue: 1
Pages: 25-30
DOI
NAID
ISSN
0912-0009, 1880-5086
Related Report
Peer Reviewed
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[Presentation] Evaluation of Antioxidative Properties of Food Factors in Relation to Inhibitory Activity of Radiation-Induced Apoptosis. The joint conference of 5th Biennial Meeting of Society for Free Radical Research-Asia (SFRR-Asia)2011
Author(s)
Ikuo Nakanishi, Hiroko P. Indo, Kei Ohkubo, Nobuo Ikota, Minako Nyui, Ken-ichiro Matsumoto, Kiyoshi Fukuhara, Shinichi Fukuzumi, Kazunori Anzai, Toshihiko Ozawa, Hideyuki J. Majima
Organizer
8th Conference for Asian Society for Mitochondrial Research and Medicine (ASMRM) and the 11th Conference of the Society of Mitochondrial Research and Medicine in Japan (J-mit)
Place of Presentation
Kagoshima, Japan
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