Roles of expressions of estrogen receptor against ROS production in estrogen-dependent diseases

• Project/Area Number: 22592094
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Kagoshima University
• Principal Investigator: SUENAGA Shigeaki 鹿児島大学, 医学部・歯学部附属病院, 講師 (00136889)
• Co-Investigator(Kenkyū-buntansha): INDO Hiroko 鹿児島大学, 医歯学総合研究科, 助教 (00301391) ; TOMITA Kazuo 鹿児島大学, 医歯学総合研究科, 助教 (60347094) ; MAJIMA Hideyuki 鹿児島大学, 医歯学総合研究科, 教授 (60165701)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 活性酸素 / エストロゲン / エストロゲンレセプター / ミトコンドリア / 過酸化脂質 / アポトーシス / アンチオキシダント効果 / オキシダント効果 / 活性酸素種 / プロオキシダント効果 / トランェクション / 蛍光免疫染色法 / Western blot法 / トランスフェクション / Western blot

Research Abstract

We examined the expressions of estrogen receptor, the production and activity of mitochondrial reactive oxygen species (ROS), lipid peroxidation and apoptosis following a cytokine plus estrogen (dose: 10-10M～10-4M) treatment in human normal fibroblasts, RA fibroblasts, VA-13, MCF-7, and MDA-MB453 cells. The oxidant and anti-oxidant effects of estrogen on the ROS generation, lipid peroxidation product, and subsequent apoptotic cell death depended on cell types, expressions of estrogen receptor (α, β), doses of estrogen.

Research Products

• [Journal Article] Roles of mitochondria-generated reactive oxygen species on X-ray-induced apoptosis in a human hepatocellular carcinoma cell line, HLE. (2012)
  • Author(s): Indo HP, Inanami O, Koumura T, Suenaga S, Yen H-C, Kakinuma S, Matsumoto K, Nakanishi I, St Clair W, St Clair DK, Matsui H, Cornette R, Gusev O, Okuda T, Nakagawa Y, Ozawa T & Majima HJ
  • Journal Title: Free Radical Research Volume: Vol. 46, No. 8 Issue: 8 Pages: 1029-1043
  • DOI: 10.3109/10715762.2012.698012
  • Peer Reviewed
• [Journal Article] Mitochondria as Possible Pharmaceutical Targets for the Effects of Vitamin E and its Homologues in Oxidative Stress-Related Diseases (2011)
  • Author(s): DOI: 10.1007/s00535-011-0434-6
  • Journal Title: Curr Pharm Des Volume: 17(21) Issue: 21 Pages: 2190-2195
  • DOI: 10.2174/138161211796957490
  • Peer Reviewed
• [Presentation] 口腔扁平上皮癌における所属リンパ節転移の画像診断精度-造影CT、USおよびPETによる評価- (2012)
  • Author(s): 末永重明、犬童寛子、川畑義裕、河野一典、佐藤強志、比地岡浩志、中村典史、仙波伊知郎、馬嶋秀行
  • Organizer: 日本歯科放射線学会 第53回学術大会
  • Place of Presentation: 盛岡
• [Presentation] 口腔扁平上皮癌における所属リンパ節転移の画像診断精度-造影CT，USおよびPETによる評価-． (2012)
  • Author(s): 末永重明，犬童寛子，川畑義裕，河野一典，佐藤強志，比地岡浩志，中村典史，仙波伊知郎，馬嶋秀行
  • Organizer: 日本歯科放射線学会 第53回学術大会
  • Place of Presentation: 盛岡
• [Presentation] Induction of mitochondrial ROS and cell death by cytokines and its prevention by estrogen in rheumatoid arthritis fibroblasts (2011)
  • Author(s): Suenaga S, Indo HP, Yen H-C, Ijiri K, Komiya S, Matsuyama T, Higuchi M, Matsui H, Ozawa T, Majima HJ
  • Organizer: 5thSFRR-Asia, 8thASMRM, 11thJ-mit 2011
  • Place of Presentation: Kagoshima
• [Presentation] Induction of mitochondrial ROS and cell death by cytokines and its prevention by estrogen in rheumatoid arthritis fibroblasts (2011)
  • Author(s): Suenaga S, Indo HP, Yen H-C, Ijiri K, Komiya S, Matsuyama T, Higuchi M, Matsui H, Ozawa T, Majima HJ
  • Organizer: 5^<th> SFRR-Asia, 8^<th> ASMRM, 11^<th> J-mit 2011
  • Place of Presentation: Kagoshima
• [Presentation] Nitric- and oxidative mitochondria related cell death by cytokines and its prevention by estrogen in estrogen receptor expressed rheumatoid arthritis synovial fibroblasts (2010)
  • Author(s): Suenaga S, Indo HP, Tomita K, Ijiri K,Komiya S, Yen H-C, Higuchi M, MatsuiH., Ozawa T, Majima HJ
  • Organizer: The 6thInternational Conference Biology,Chemistry, and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: Kyoto
• [Presentation] Nitric- and oxidative mitochondria related cell death by cytokines and its prevention by estrogen in estrogen receptor expressed rheumatoid arthritis synovial fibroblasts. (2010)
  • Author(s): Suenaga S, Indo HP, Tomita K, Ijiri K, Komiya S, Yen H-C, Higuchi M, Matsui H, Ozawa T, Majima HJ.
  • Organizer: The 6^<th> International Conference Biology, Chemistry, and Therapeutic Applications of Nitric Oxide
  • Place of Presentation: Kyoto