Effect to immunoresponse of antibacterial peptides

• Project/Area Number: 22592095
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Kagoshima University
• Principal Investigator: KANBARA Kenji 鹿児島大学, 大学院・医歯学総合研究科, 助教 (60305141)
• Co-Investigator(Kenkyū-buntansha): KOMATSUZAWA Hitoshi 鹿児島大学, 医歯学総合研究科, 教授 (90253088)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: LL-37 / LL37 / DNA / TLR9 / 歯周病態形成 / サイトカイン / TLR-9

Research Abstract

In this study, it was demonstrated that LL-37 interacted with bacterial DNA/RNA and these complexes were tolerated to several nuclease. Furthermore, LL-37 interacted to apolipoprotein-A1(Apo-A1) which is a component of high density lipoprotein. It was considered that these complexes stimulate immune system effectively for a long time and LL-37-Apo-A1 complexes may be associated between metabolic syndrome. Those complexes tend to increase expression of CXCL10 in gingival epithelia cells, Ca9-22 cells. However, we have been not confirming increase of CXCL10 protein secretion level yet.

Research Products

• [Journal Article] Antimicrobial peptide LL-37 promotes vascular endothelial growth factor-A expression in human periodontal ligament cells (2013)
  • Author(s): Kittaka M, Shiba H, Kajiya M, Ouhara K, Takeda K, Kanbara K, Fujita T, Kawaguchi H, Komatsuzawa H, Kurihara H
  • Journal Title: J Periodontal Res Volume: 48(2) Pages: 228-34
  • URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0765.2012.01524.x/pdf
  • Peer Reviewed
• [Journal Article] Antimicrobial peptide LL37 promotes vascular endothelial growth factor-A expression in human periodontal ligament cells. (2013)
  • Author(s): Kittaka M, Shiba H, Kajiya M, Ouhara K, Takeda K, Kanbara K, Fujita T, Kawaguchi H, Komatsuzawa H, Kurihara H.
  • Journal Title: J Periodontal Res. Volume: 48(2) Pages: 228-34
  • Peer Reviewed
• [Journal Article] Association of CiaRH with resistance of Streptococcus mutans to antimicrobial peptides in biofilms (2012)
  • Author(s): Mazda Y, Kawada-Matsuo M, Kanbara K, Oogai Y, Shibata Y, Yamashita Y, Miyawaki S, Komatsuzawa H
  • Journal Title: Mol Oral Microbiol Volume: 27(2) Pages: 124-35
  • URL: http://onlinelibrary.wiley.com/doi/10.1111/j.2041-1014.2012.00637.x/pdf
  • Peer Reviewed
• [Journal Article] Association of CiaRH with resistance of Streptococcus mutans to antimicrobial peptides in biofilms. (2012)
  • Author(s): Mazda Y, Kawada-Matsuo M, Kanbara K, Oogai Y, Shibata Y, Yamashita Y, Miyawaki S, Komatsuzawa H.
  • Journal Title: Mol Oral Microbiol. Volume: 27(2) Pages: 124-35
  • Peer Reviewed
• [Presentation] 汗中の抗菌性ペプチドの定量 (2012)
  • Author(s): 神原賢治
  • Organizer: 細菌学会総会
  • Place of Presentation: 長崎ブリックホール