Inhibition of NFκB activity by MALT1 and its involvement in phenotypic alterations of oral carcinoma cells

• Project/Area Number: 22592103
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: The Nippon Dental University
• Principal Investigator: IMAI Kazushi 日本歯科大学, 生命歯学部, 教授 (10328859)
• Co-Investigator(Kenkyū-buntansha): CHIBA Tadashige 日本歯科大学, 生命歯学部, 准教授 (60350138) ; MORIKAWA Masako 日本歯科大学, 生命歯学部, 准教授 (80095265)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
• Keywords: 口腔癌 / MALT1

Research Abstract

The present study examined a role of mucosa-associated lymphoid tissue 1 (MALT1), whose expression is inactivated in oral carcinomas with worse prognosis, in oral carcinoma cells and its involvement in phenotypic alteration of the cells by the microarray and proteomic analyses. MALT1 down-regulated expression of genes in the TGF-ss and EGF pathways, alternated keratin species involved in aggressive feature of carcinoma cells, and suppressed proliferation and migration of the cells. Furthermore, E-cadherin-mediated carcinoma cell-cell adhesion machinery, which was also strongly down-regulated by MALT1, was rapidly obstructed with progression of oral carcinomas.

Research Products

• [Journal Article] 口腔癌進展のメカニズムと分子標的薬 (2013)
  • Author(s): 今井一志、須藤遥、前田元太、千葉忠成
  • Journal Title: 歯学 Volume: 100 Pages: 177-181
• [Journal Article] Single nucleotide polymorphisms of mucosa-associated lymphoid tissue 1 in oral carcinoma cells and gingival fibroblasts. (2013)
  • Author(s): 大山豪
  • Journal Title: Odontology Volume: 印刷中 Issue: 2 Pages: 150-155
  • DOI: 10.1007/s10266-012-0079-9
  • Peer Reviewed
• [Journal Article] Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-asociated lymphoid tissue 1 expression (2013)
  • Author(s): 川本寛之
  • Journal Title: Int J Oncol Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Progression of oral squamous cell carcinoma accompanied with reducedE-cadherin expression but not cadherin switch (2012)
  • Author(s): Hashimoto T., Soeno Y., Maeda H., Taya Y., Aoba T., Nasu M., Kawashiri S., Imai K
  • Journal Title: PLOS ONE Volume: 7 Issue: 10 Pages: 1-8
  • DOI: 10.1371/journal.pone.0047899
  • Peer Reviewed
• [Journal Article] Mutational analysis of HRAS and KRAS genes in oral carcinoma cell lines (2011)
  • Author(s): Maemoto S, et al
  • Journal Title: Odontology Volume: (印刷中) Issue: 2 Pages: 149-155
  • DOI: 10.1007/s10266-011-0032-3
  • Year and Date: 2011-05-24
  • Peer Reviewed
• [Journal Article] Concomitant loss of p120-catenin and β-catenin membrane expression and oral carcinoma progression with E-cadherin reduction.
  • Author(s): Sasaya K, Sudo H, Maeda G, Kawashiri S, Imai K.
  • Journal Title: PLoS One Volume: (in press)
  • URL: http://www.plosone.org/home.action
  • Peer Reviewed
• [Journal Article] Proteomic identification of keratin alterations with enhanced proliferation of oral carcinoma cells by loss of mucosa-associated lymphoid tissue 1 expression.
  • Author(s): Kawamoto Y, Ohyama Y, Chiaba T, Yagishita H, Sakashita H, Imai K.
  • Journal Title: Int J Oncol Volume: (in press)
  • URL: http://www.spandidos-publications.com/ijo/
  • Peer Reviewed
• [Journal Article] Inhibition of TGF- and EGF pathway gene expression and migration of oral carcinoma cells by mucosa-associated lymphoid tissue 1.
  • Author(s): Ohyama Y, Kawamoto Y, Chiba T, Maeda G, Sakashita H, Imai K
  • Journal Title: Br J Cancer Volume: (in press)
  • Peer Reviewed
• [Journal Article] Single nucleotide polymorphisms of mucosa-associated lymphoid tissue 1 n oral carcinoma cells and fibroblasts.
  • Author(s): Oyama G, Midorikawa T, Matsumoto Y, Takeyama M, Yamada K, Nozawa T, Morikawa M, Imai K.
  • Journal Title: Odontology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Mutational analysis of HRAS and KRAS genes in oral carcinoma cell lines
  • Author(s): Maemoto S, et al.
  • Journal Title: Odontology Volume: (印刷中)
  • Peer Reviewed
• [Presentation] MALT1-responsive keratin rearrangement and proliferation of oral carcinoma cells. (2013)
  • Author(s): Kawamoto H, Ohyama Y, Chiba T, Yagishita H, Sakashita H, Imai K.
  • Organizer: IADR/AADR/CADR General Session and Exhibition
  • Place of Presentation: Seattle, WA,USA
• [Presentation] Profiling and network analysis of MALT1-responsive gene datasets in SCC. (2013)
  • Author(s): Ohyama Y, Kawamoto H, Chiba T, Maeda G, Sakashita H, Imai K.
  • Organizer: IADR/AADR/CADR General Session and Exhibition
  • Place of Presentation: Seattle,WA,USA
• [Presentation] MALT1-responsive keratin rearrangement and proliferation of oral carcinoma cells (2013)
  • Author(s): 今井一志
  • Organizer: IADR General Session and Exhibition
  • Place of Presentation: Seatle, USA
• [Presentation] Profiling and network analysis of MALT1-responsive gene dataset in SCC (2013)
  • Author(s): 今井一志
  • Organizer: IADR General Session and Exhibition
  • Place of Presentation: Seatle, USA
• [Presentation] 口腔癌の進展にはカドヘリンスイッチではなく、E-カドヘリンの発現低下が関連する (2012)
  • Author(s): 橋本孝志、添野雄一、田谷雄二、青葉孝昭、那須優則、前田元太、須藤遥、千葉忠成、今井一志
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山市
  • Year and Date: 2012-09-15
• [Presentation] 口腔癌におけるp120カテニンとβカテニン発現の免疫組織学的解析 (2012)
  • Author(s): 笹谷和伸、前田元太、須藤遥、千葉忠成、今井一志
  • Organizer: 第54回歯科基礎医学会学術大会・総会
  • Place of Presentation: 郡山市
  • Year and Date: 2012-09-15
• [Presentation] Progression/metastasis of xenografted oral cancer cell-lines in mouse tongue. (2012)
  • Author(s): Soeno Y, Shirako Y, Fujita K, Taya Y, Shimazu Y, Nakau K, Sato K, Chiba T, ImaiK,AobaT
  • Organizer: 41st Annual Meeting & Exhibition of the AADR
  • Place of Presentation: Tampa,FL,USA
• [Presentation] 口腔癌の進展にはカドヘリンスイッチではなく、E-カドヘリンの発現低下が関連する (2012)
  • Author(s): 今井一志
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 郡山市
• [Presentation] 口腔癌におけるp120カテニンとbeta-カテニン発現の免疫組織学的解析 (2012)
  • Author(s): 今井一志
  • Organizer: 歯科基礎医学会
  • Place of Presentation: 郡山市
• [Presentation] 口腔扁平上皮癌細胞におけるMALT1誘導タンパク質のプロテオーム解析 (2011)
  • Author(s): 川本幸寛, 他
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 長良川国際会議場(岐阜県)
  • Year and Date: 2011-10-01
• [Presentation] 口腔扁平上皮癌細胞におけるMALT1誘導タンパク質のプロテオーム解析 (2011)
  • Author(s): 川本幸寛、大山嘉人、千葉忠成、坂下英明、今井一志
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜市
• [Presentation] 口腔癌細胞におけるMALT1誘導性遺伝子のマイクロアレイ解析
  • Author(s): 今井一志
  • Organizer: 日本生化学会
  • Place of Presentation: 福岡市
• [Book] Chapter 7: Cadherin expression and progression of squamous cell carcinomas of the oral cavity. In Squamous Cell Carcinom (2012)
  • Author(s): Imai K, Maeda G, Chiba T
  • Publisher: Li X,,ed